Investigation of the Effect of Local Electrical Stimulation on Cells Cultured on Conductive Single-Walled Carbon Nanotube/Albumin Films

Abstract

In this study we have developed a biocompatible current-conductive coating based on carbon nanotubes and bovine serum albumin and have shown its efficiency in culturing cells in vitro. We investigate the proliferation of human embryonic fibroblast (HEF) cells, which were subjected to electrical stimulation when cultured on carbon nanotube surface. A weak increase in proliferation is demonstrated at stimulating field pulses up to 100 mV. It is assumed that the transport mechanism accompanied by higher synthesis of proteins and their polymerization may increase proliferative activity at low voltages. At higher voltages the motility and spatial organization of HEF cell is observed. As a result, a novel technique of supplying the cells with electric field through a system of micro- and nanosized electrodes and a biocompatible composite have been developed

Two-Photon Polymerization of Albumin Hydrogel Nanowires Strengthened with Graphene Oxide

“…Previously we demonstrated that CNT/BSA composites with varied conductivity and concentration can be used as biocompatible materials for sensor application [ 14 ] or scaffold for cells growth [ 15 ]. The 2D materials, such as graphene, provide advanced properties of composites for lower load concentration while maintaining flexibility and stretchable properties.…”