Investigation of the effect of hesperidin on some reproductive parameters in testicular toxicity induced by B isphenol A

Abstract

Bisphenol A (BPA) is one of the chemicals that cause dysfunction and infertility in testicles. Therefore, it is crucial to develop effective treatments against this damage. In this study, the effects of Hesperidin (HESP), a flavonoid in testicular toxicity induced by BPA in rats, on oxidative stress, inflammation, apoptosis, histological damage, spermatogenesis, steroidogenic enzymes and reproductive hormones were investigated. Our study used 52 Sprague Dawley male rats weighing 250–300 g, and four experimental groups were formed. From the experimental groups, 1 ml of olive oil was administered to the control group, HESP at a dose of 50 mg/kg to the HESP group, BPA at a dose of 100 mg/kg to the BPA group, HESP at a dose of 50 mg/kg to the BPA + HESP group and 100 mg/kg BPA was administered intragastrically (ig) for 14 days. We determined that BPA administration causes apoptosis, histological damage, inflammation, oxidative stress and toxic effects on spermatogenesis and steroidogenic enzymes in testicles. We observed that the administration of HESP with BPA attenuated oxidative stress, inflammation and apoptosis resulting in therapeutic effects on both steroidogenic enzymes and spermatogenesis and reproductive hormones (FSH, LH and testosterone). Our findings from this study clearly showed that while HESP treatment alleviates oxidative damage, inflammation and apoptosis in testicles of rats treated with BPA, it has regulatory effects on steroidogenic enzymes, spermatogenesis and serum reproductive hormones.

The Palliative and Antioxidant Effects of Hesperidin against Lead-Acetate-Induced Testicular Injury in Male Wistar Rats

“…Nonetheless, a substantial increase in the serum levels of FSH, LH, and testosterone was observed in PbAc-intoxicated rats pre-treated with HSD. Knowing that OS can lower FSH, LH, and testosterone levels [ 129 ], the restoration of FSH, LH, and/or testosterone levels mediated via HSD might be attributed to its antioxidant effect as previously reported [ 88 , 122 ].…”

Eltroxin and Hesperidin mitigate testicular and renal damage in hypothyroid rats: amelioration of oxidative stress through PPARγ and Nrf2/HO-1 signaling pathway

“…Similarly, treatment of CBZ-induced rats with HSP markedly decreased renal and testicular IL-6 and TNF-α levels, indicating mitigated inflammation. Previous studies showed the anti-inflammatory effect of HSP in renal tissues [ 16 , 76 ] and testicular tissues [ 77 ]. Under HPO conditions, HSP has been indicated to decrease inflammation in liver and lung as previously reported [ 51 , 78 ].…”

“…Co-administration of ELT or HSP showed improvement in testicular structure as previously reported [ 61 , 87 ]. Their protective effect on HPO-induced testicular degeneration may be attributed to the antioxidant abilities, which eliminate free radicals to minimize lipid peroxidation and therefore preserve normal histological structure of testes [ 77 , 113 ].…”

Selenium reduces acrylamide‐induced testicular toxicity in rats by regulating HSD17B1, StAR, and CYP17A1 expression, oxidative stress, inflammation, apoptosis, autophagy, and DNA damage

“…Sperm motility was determined by visual estimation for each rat. At least two different areas were evaluated, and the mean motility score was recorded as the final motility score 30 …”

“…At least two different areas were evaluated, and the mean motility score was recorded as the final motility score. 30 To determine sperm density, semen samples were diluted 1/100 with physiological saline in a centrifuge tube. Tubes were vortexed at 2500 rpm for 15 s. Approximately 20 μL of semen was transferred to the Thoma counting chamber.…”