Investigation of the Effect of Humic Acid on Bone Regeneration in Parietal Defects Formed in Rats Ratlarda Oluşturulan Pari̇etal Defektlerde Humi̇k Asi̇ti̇n Kemi̇k Rejenerasyonu Üzeri̇ne Etki̇si̇ni̇n Araştirilmasi

Abstract

Objectives:The objective of our study was to investigate the effect of the local humic acid application in addition to autogenous bone grafting on the premature new bone amount.Materials and Methods: 12-week-old 24 Wistar rats were divided into 4 groups.Two parietal bone defects with 5-mm diameter were constituted in each animal. All defects in each group were treated with only autogenous bone graft (n=6), autogenous bone graft combined with 100 mg/kg (n=6) or 200 mg/kg humic acid (n=6). Defects in one group remained empty as control (n=6). The laboratory animals were sacrificed on the 28th day following the procedure. Morphological properties of all experimental defects were evaluated using micro-computed tomography (Micro-CT). Results:The highest value among trabecular thickness (Tb.Th) and the ratio of the bone volume to the tissue volume (BV/ TV) was encountered in the autogenous graft group applied with 100mg/kg humic acid. The highest value in the bone volume (BV) variance was detected in the group to which only the autogenous graft was applied. A statistically significant difference was found between the control group and the only autogenous graft-applied group and the autogenous graft group applied with local 100mg/kg humic acid upon comparing the groups in pairs for bone surface area (BS) variance. BS/BV value was found higher in the autogenous graft group applied with local 200mg/kg humic acid than the autogenous group applied with local 100mg/kg humic acid.Conclusions: Humic acid application in addition to autogenous bone grafting caused a decrease in the bone volume value. However, the positive effect of the humic acid application was observed in trabecular thickness and bone volume/tissue volume values, dosage increase negatively impacted the same. The dosage increase negatively affected the bone surface area value. No positive effect of the humic acid application to bone surface/bone volume value was observed. The statistical significance between the control group and the other groups is considered to be autogenous graft.