Investigation of the effect of poorly controlled diabetes mellitus on erythrocyte life

Sayinalp, Sabri; Sözen, Tümay; Usman, Aydan; Dündar, Semra

doi:10.1016/1056-8727(94)00041-l

Cited by 14 publications

(16 citation statements)

References 15 publications

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“…Glycation of albumin to minimal extents, as found in diabetes in vivo, has no significant effect on albumin half-life [18]. Similarly, little change has been observed in the lifespan of red blood cells (RBCs) in diabetic patients vs control subjects [19]. Accordingly, the changes in glycation, oxidation and nitration adduct residues in plasma protein and haemoglobin in the diabetic subjects of this study are not due to changes in protein substrate turnover but rather to changes in protein modification.…”

Section: Discussionmentioning

confidence: 99%

Degradation products of proteins damaged by glycation, oxidation and nitration in clinical type 1 diabetes

et al. 2005

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Aims/hypothesis: Hyperglycaemia in diabetes is associated with increased glycation, oxidative stress and nitrosative stress. Proteins modified consequently contain glycation, oxidation and nitration adduct residues, and undergo cellular proteolysis with release of corresponding free adducts. These free adducts leak into blood plasma for eventual renal excretion. The aim of this study was to perform a comprehensive quantitative analysis of protein glycation, oxidation and nitration adduct residues in plasma protein and haemoglobin as well as of free adducts in plasma and urine to quantify increased protein damage and flux of proteolytic degradation products in diabetes. Methods: Type 1 diabetic patients (n=21) and normal healthy control subjects (n=12) were studied. Venous blood samples, with heparin anticoagulant, and 24-h urine samples were taken. Samples were analysed for protein glycation, oxidation and nitration adducts by a quantitative comprehensive screening method using liquid chromatography with triple quadrupole mass spectrometric detection. Results: In type 1 diabetic patients, the concentrations of protein glycation, oxidation and nitration adduct residues increased up to three-fold in plasma protein and up to one-fold in haemoglobin, except for decreases in pentosidine and 3-nitrotyrosine residues in haemoglobin when compared with normal control subjects. In contrast, the concentrations of protein glycation and oxidation free adducts increased up to ten-fold in blood plasma, and urinary excretion increased up to 15-fold in diabetic patients. Conclusions/interpretation: We conclude that there are profound increases in proteolytic products of glycated and oxidised proteins in diabetic patients, concurrent with much lower increases in protein glycation and oxidation adduct residues.

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Section: Discussionmentioning

confidence: 99%

Degradation products of proteins damaged by glycation, oxidation and nitration in clinical type 1 diabetes

et al. 2005

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“…However, even modest variation in red cell survival, that would not be apparent in routine hematological studies, could have a significant impact on HbA1c. Studies of RBC survival in diabetes are not consistent, showing both unaltered [67,70] and decreased [71,72] RBC survival. Recent data obtained using the biotin label in a small number of diabetic subjects [61,65] indicate that although RBC lifespan does not differ significantly between diabetic and control groups, the range of mean RBC age (40-52 days in the diabetic group) is sufficient to have a significant impact on the percentage of HbA1c.…”

Section: Diabetesmentioning

confidence: 94%

The measurement and importance of red cell survival

2008

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The measurement of red blood cell survival in the circulation has progressed from the original differential agglutination technique of Ashby to current isotopic and flow cytometric methods. While occasionally useful in the clinic, these methods find widespread use in a number of important research areas, including the evaluation of new red cell storage media in transfusion medicine and studies of the pathophysiology of sickle cell disease and diabetes. In this review, measurement techniques are placed in historical perspective and examined for relative merits and suitable application. Am. J. Hematol. 84:109-114, 2009. V

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“…These results differ from 2 previous reports 8,9 but agree with others. 10,12 It is important, in examining these results, to consider the potential sources of variation. The individual survival curves appear to be very precise (R 2 Ͼ 0.99), and repeated assays at a given time point agreed closely.…”

Section: Rbc Life Spanmentioning

confidence: 99%

“…4,5 However, normal RBC life span has been reported to have a wide range of values regardless of the method used, [6][7][8] and there are also studies suggesting that RBC life span may depend on glycemic control, although evidence for this is conflicting. [9][10][11][12][13] It is also possible that heterogeneity in other features of red cell physiology, such as membrane glucose transport 14 and loss of Hb from older RBCs, 15 may influence the relationship between blood glucose and HbA1c.…”

Section: Introductionmentioning

confidence: 99%

“…Given the discordance between HbA1c and other measures of glycemic control observed in some subjects [31][32][33][34] and the evidence for a range of RBC survival in previous studies, 9,10,12,35 we sought to determine whether there is sufficient interindividual variation in RBC mean age to have an important effect on HbA1c. To maximize the probability of observing a wide range of life spans in a small number of subjects, patients with diabetes mellitus (DM) with discordance between HbA1c and serum fructosamine (FA) were selected.…”

Section: Introductionmentioning

confidence: 99%

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Red cell life span heterogeneity in hematologically normal people is sufficient to alter HbA1c

Cohen

Franco²,

Khera

et al. 2008

Blood

390

370

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Although red blood cell (RBC) life span is a known determinant of percentage hemoglobin A1c (HbA1c), its variation has been considered insufficient to affect clinical decisions in hematologically normal persons. However, an unexplained discordance between HbA1c and other measures of glycemic control can be observed that could be, in part, the result of differences in RBC life span. To explore the hypothesis that variation in RBC life span could alter measured HbA1c sufficiently to explain some of this discordance, we determined RBC life span using a biotin label in 6 people with diabetes and 6 nondiabetic controls.

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Investigation of the effect of poorly controlled diabetes mellitus on erythrocyte life

Cited by 14 publications

References 15 publications

Degradation products of proteins damaged by glycation, oxidation and nitration in clinical type 1 diabetes

Degradation products of proteins damaged by glycation, oxidation and nitration in clinical type 1 diabetes

The measurement and importance of red cell survival

Red cell life span heterogeneity in hematologically normal people is sufficient to alter HbA1c

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