Investigation of the Effective Action Distance Between Hematopoietic Stem/Progenitor Cells and Human Adipose-Derived Stem Cells During Their In Vitro Co-culture

Song, Kedong; Wang, Hai; Wang, Hong; Wang, Ling; Qiao, Mo; Wu, Shuang; Liu, Tianqing

doi:10.1007/s12010-011-9295-y

Cited by 9 publications

(6 citation statements)

References 29 publications

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“…The morphological changes of the cultured cells were observed under a microscope (Olympus, Japan). The samples were stained by oil red [22,23] and toluidine blue [24][25][26] (Sigma, USA) after 2 weeks of inductions.…”

Section: Cell Morphology and Multiple Differentiation Potential Of Adscsmentioning

confidence: 99%

Dynamic Fabrication of Tissue-Engineered Bone Substitutes Based on Derived Cancellous Bone Scaffold in a Spinner Flask Bioreactor System

Song

Zhu

et al. 2014

Appl Biochem Biotechnol

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The in vitro dynamic fabrications of tissue-engineered bones were performed to assess the advantages of human adipose-derived stem cells (hADSCs) combined with acellular cancellous bone scaffold coming from fresh pig femur in a spinner flask compared with traditional static culture. In this study, the bio-derived cancellous bone was regarded as a biomimetic scaffold, and its surface appearance was observed under scanning electron microscopy (SEM). Moreover, its modulus of elasticity and chemical composition were measured with universal testing machine (UTM) and infrared detector, respectively. hADSCs were inoculated into cancellous bone scaffold at a density of 1 × 10(6) cells/mL and cultured in spinner flask and T-flask with osteogenic medium (OM) for 2 weeks, respectively. Following to this, the osteogenic differentiation was qualitatively and quantitatively detected with alkaline phosphatase (ALP) kits, and the cell growth and viability were assayed using Live/Dead staining; cell adhesion and extracellular matrix secretion were observed under a SEM. The average pore size of cancellous bone scaffold was 284.5 ± 83.62 μm, the elasticity modulus was 41.27 ± 15.63 MPa, and it also showed excellent biocompatibility. The hADSCs with multidifferentiation potentials were well proliferated, could grow to 90 % fusion within 5 days, and were therefore suitable to use as seed cells in the construction of tissue-engineered bones. After 2 weeks of fabrication, cells were well-distributed on scaffolds, and these scaffolds still remained intact. Compared to static environment, the ALP expression, cell distribution, and extracellular matrix secretion on cancellous bones in spinner flask were much better. It confirmed that three-dimensional dynamic culture in spinner flask promoted ADSC osteogenic differentiation, proliferation, and matrix secretion significantly to make for the fabrication of engineered bone substitutes.

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Section: Cell Morphology and Multiple Differentiation Potential Of Adscsmentioning

confidence: 99%

Dynamic Fabrication of Tissue-Engineered Bone Substitutes Based on Derived Cancellous Bone Scaffold in a Spinner Flask Bioreactor System

Song

Zhu

et al. 2014

Appl Biochem Biotechnol

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“…Diffusion assays are one type of application that could benefit from this functionality. These assays typically require the ability to control the location and timing of reagent delivery [63]: for example, to control cell-cell communication and phenotypic responses in cellular co-culture systems [64,65].…”

Section: Sample-in-sample Delivery With Spatiotemporal Controlmentioning

confidence: 99%

Digital Microfluidic System with Vertical Functionality

Bender

Garrell

2015

Micromachines

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Digital (droplet) microfluidics (DµF) is a powerful platform for automated lab-on-a-chip procedures, ranging from quantitative bioassays such as RT-qPCR to complete mammalian cell culturing. The simple MEMS processing protocols typically employed to fabricate DµF devices limit their functionality to two dimensions, and hence constrain the applications for which these devices can be used. This paper describes the integration of vertical functionality into a DµF platform by stacking two planar digital microfluidic devices, altering the electrode fabrication process, and incorporating channels for reversibly translating droplets between layers. Vertical droplet movement was modeled to advance the device design, and three applications that were previously unachievable using a conventional format are demonstrated: (1) solutions of calcium dichloride and sodium alginate were vertically mixed to produce a hydrogel with a radially symmetric gradient in crosslink density; (2) a calcium alginate hydrogel was formed within the through-well to create a particle sieve for filtering suspensions passed from one layer to the next; and (3) a cell spheroid formed using an on-chip hanging-drop was retrieved for use in downstream processing. The general capability of vertically delivering droplets between multiple stacked levels represents a processing innovation that increases DµF functionality and has many potential applications.

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“…A variety of stromal cells have been studied as matrices for ex vivo expansion of HSC, including MSCs isolated from bone marrow [80][81][82], adipose tissue [83][84][85], placenta [86], umbilical cord blood [87][88][89], umbilical cord tissue (Wharton's Jelly) [90][91][92][93][94], and fetal liver [95]. Bone marrow-derived mesenchymal stem cells have been widely used and clinical-scale UCBMNC-BMMSC in vitro co-culture procedures were well developed [96] and validated [97].…”

Section: Co-culture With Stromal Cellsmentioning

confidence: 99%

Umbilical Cord Blood Hematopoietic Stem Cell Expansion Ex Vivo

Ren¹,

Jiang²

2013

J Blood Disord Transfus 2012

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Umbilical cord blood is an attractive source of hematopoietic stem and progenitor cells in the treatment of hematologic diseases, especially in allogeneic hematopoietic cell transplantation. However, due to the low abundance of these cells, the therapeutic use of umbilical cord blood has been limited mostly to the pediatric setting. The strategies for adult umbilical cord blood transplantation have been improved, with the recent development of various approaches for expanding stem cells in vitro and enhancing their long-term homing efficiency. In this brief review, we discuss a number of strategies for stimulating the proliferation of umbilical cord blood hematopoietic stem cells in vitro, including the utility of transcription factors and growth factors (cytokine cocktails), as well as co-culturing with stromal cells. Ultimately, we make the case that improvements in umbilical cord blood stem cell expansion will be critical for enhancing transplantation engraftment efficacy and providing potential cure for hematological diseases.

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Investigation of the Effective Action Distance Between Hematopoietic Stem/Progenitor Cells and Human Adipose-Derived Stem Cells During Their In Vitro Co-culture

Cited by 9 publications

References 29 publications

Dynamic Fabrication of Tissue-Engineered Bone Substitutes Based on Derived Cancellous Bone Scaffold in a Spinner Flask Bioreactor System

Dynamic Fabrication of Tissue-Engineered Bone Substitutes Based on Derived Cancellous Bone Scaffold in a Spinner Flask Bioreactor System

Digital Microfluidic System with Vertical Functionality

Umbilical Cord Blood Hematopoietic Stem Cell Expansion Ex Vivo

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