Investigation of the effects of Lycium barbarum polysaccharides against cadmium induced damage in testis

Varoni, Maria Vittoria; Gadau, Sergio Domenico; Pasciu, Valeria; Baralla, Elena; Serra, Elisa; Palomba, D.; Demontis, Maria Piera

doi:10.1016/j.yexmp.2017.06.003

Cited by 22 publications

(15 citation statements)

References 34 publications

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“…Lycium barbarum polysaccharides (LBP) is the active ingredient extracted from Lycium barbarum is, a plant species which produces the wolfberry and a traditional Chinese medicine, and has beneficial effects, particularly in the liver, kidney and eyes ( 18 , 19 ). Recent reports have demonstrated that LBP effectively improves immune function, resists oxidative free radicals and protects the testes from high-temperature injury ( 20 ).…”

Section: Introductionmentioning

confidence: 99%

Lycium�barbarum polysaccharides protect human trophoblast HTR8/SVneo cells from hydrogen peroxide‑induced oxidative stress and apoptosis

Ding

Yang

et al. 2018

Mol Med Report

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Pregnancy complications are associated with abnormal cytotrophoblast differentiation and invasion. Hydrogen peroxide (H2O2) is an important mediator of oxidative ischemia/reperfusion stress in the placenta. Lycium barbarum polysaccharides (LBP) have been demonstrated to counteract oxidative free radicals. The effects of LBP in trophoblast HTR8/SVneo cells injured with H2O2 were examined. A cell counting kit-8 assay was performed to detect the effect of LBP at different concentrations on the proliferative ability of H2O2 injured trophoblast cells. Flow cytometry was used to determine the levels of reactive oxygen species (ROS), mitochondria membrane potential (MMP) disruption and apoptosis. Superoxide dismutase (SOD) activity and lactate dehydrogenase (LDH) leakage into the supernatant was detected by ultraviolet spectrophotometry. Reverse transcription-quantitative polymerase chain reaction and western blot analysis were performed to detect the expression of apoptosis-associated factors, including survivin, hypoxia inducible factor 1-α (HIF1-α), Bcl-2 apoptosis regulator (Bcl-2), Bcl-2 associated X apoptosis regulator (Bax). The results revealed that LBP protected the proliferative ability of trophoblast cells injured with H2O2 in a dose-dependent manner. LBP inhibited the oxidative stress induced by H2O2, by reducing ROS and LDH levels and increasing SOD activity. Additionally, LBP decreased MMP disruption and cell apoptosis induced by H2O2, by increasing the mRNA and protein expression of survivin, HIF1-α and Bcl-2 and decreasing Bax expression. Therefore, it was concluded that LBP protected human trophoblast cells from H2O2-induced oxidative stress and cell apoptosis via regulation of apoptosis-associated factor expression. It will provide a novel strategy for the treatment of pregnancy complications.

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Section: Introductionmentioning

confidence: 99%

Lycium�barbarum polysaccharides protect human trophoblast HTR8/SVneo cells from hydrogen peroxide‑induced oxidative stress and apoptosis

Ding

Yang

et al. 2018

Mol Med Report

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“…57 Oligospermia is a fertility situation which is characterized by low sperm count. Different heavy metals which have been known to facilitate testicular damage include cadmium 58,59 and mercury. 60 These metals can alter certain oxidative stress parameters, the weight of the testes and its morphology, thus lowering the chances of fertility in men.…”

Section: Testicular Damagementioning

confidence: 99%

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2020

TJNPR

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contaminants in the environmental systems are the heavy metals which gain access into our environment through natural and human activities such as soil erosion and runoffs , mining and rock weathering, indiscriminate disposal of industrial effluents, sewage discharge, unguided use of pesticides, etc. 1 Although some of these metals are "essential", however, above their permissible limits, such metals become harmful to the body. The WHO permissible limit for cadmium, chromium, copper, lead, manganese and nickel in drinking water are 0.003, 0.05, 2.00, 0.01, 0.50, and 0.02 mg/L, respectively. 2 Above these levels, these metals are no longer beneficial. When aquatic animals ingest heavy metal-contaminated water, they naturally bio-accumulate the metals. Similarly, when plants are irrigated with such water, heavy metal accumulation occurs. The eventual consumption of these is dangerous as it consequently leads to the development of various life-threatening diseases such as cancer and organ damage among others. The threat of heavy metal poisoning is higher for people in rural areas. Particularly, people in neglected parts of developing nations are more prone to such poisoning because of ignorance and lack of potable water supply. 3

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“…e glucoside apart, which is composed of arabinose, rhamnose, xylose, mannose, galactose, and glucose (Figure 1), accounts for more than 90% of the LBP mass [28]. Evidence shows LBP could ameliorate testicular damage by upregulating the testosterone level and reducing germ cell apoptosis [29][30][31][32]. However, limited research has been conducted on how LBP influences SCs and BTB.…”

Section: Introductionmentioning

confidence: 99%

Lycium barbarum Polysaccharide Ameliorates Heat-Stress-Induced Impairment of Primary Sertoli Cells and the Blood-Testis Barrier in Rat via Androgen Receptor and Akt Phosphorylation

Liu

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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Male infertility induced by heat stress has been attracting more and more attention. Heat stress not only causes apoptosis of spermatocytes but also has adverse effects on Sertoli cells, further damaging spermatogenesis. Lycium barbarum polysaccharide (LBP) is the main bioactive component of Lycium barbarum, which has a protective effect on male reproduction, but its mechanism is still unclear. In this study, our results proved that LBP blocked the inhibitory effect on the proliferation activity of Sertoli cells after heat stress, reversed the dedifferentiation of Sertoli cells induced by heat stress, and ameliorated the structural integrity of the blood-testis barrier. In addition, it increased the expression of the androgen receptor and activated Akt signaling pathway to resist heat-stress-induced injury of Sertoli cells.

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Investigation of the effects of Lycium barbarum polysaccharides against cadmium induced damage in testis

Cited by 22 publications

References 34 publications

Lycium�barbarum polysaccharides protect human trophoblast HTR8/SVneo cells from hydrogen peroxide‑induced oxidative stress and apoptosis

Lycium�barbarum polysaccharides protect human trophoblast HTR8/SVneo cells from hydrogen peroxide‑induced oxidative stress and apoptosis

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Lycium barbarum Polysaccharide Ameliorates Heat-Stress-Induced Impairment of Primary Sertoli Cells and the Blood-Testis Barrier in Rat via Androgen Receptor and Akt Phosphorylation

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