Investigation of the effects of 50 Hz magnetic fields on platelet aggregation using a modified aggregometer

Sağdilek, Engin; Sebik, Oğuz; Çelebi, Gürkan

doi:10.3109/15368378.2012.681822

Cited by 6 publications

(7 citation statements)

References 26 publications

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“…The experimental setup in our previous study [Sağdilek et al, 2012] was one that evaluated the electromagnetic field effects after the exposure. Platelets were exposed to 1 mT, 50 Hz magnetic fields for 1.5 h using Helmholtz coils and aggregations were evaluated using a modified aggregometer.…”

Section: Discussionmentioning

confidence: 99%

“…With the help of this motor and magnet, the stir bar inside the PRP is spun, thus stirring the PRP sample (Fig. 1a from Sağdilek et al [2012]).…”

Section: Methodsmentioning

confidence: 99%

“…The magnets attached to the DC motors in the aggregometer were removed. The modified aggregometer configuration and a photograph of the setup are shown in Figures 1b and 2 [Sağdilek et al, 2012].…”

Section: Methodsmentioning

confidence: 99%

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Does the magnetic field of a magnetic stirrer in an optical aggregometer affect concurrent platelet aggregation?

Sağdilek

Sebik

Çelebi

2013

Bioelectromagnetics

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Platelets are subjected to extremely low frequency electromagnetic fields during standard aggregometry measurements owing to the use of a magnetic stir bar in the instrument. This study evaluates the effects of this magnetic field exposure on platelet aggregation by comparing the results obtained in a modified aggregometer. Blood samples from healthy volunteers were anticoagulated using citrate or heparin. Platelet-rich plasma (PRP) samples were prepared. A mechanical stirring device was attached to the aggregometer instead of the magnetic stir bar system. The PRP samples were stirred using a stirring rod tip that did not produce any magnetic fields in one channel of the aggregometer; in the other channel, a stirring rod carrying a small magnet at its tip was used. As a result, a magnetic field in the extremely low frequency range and in the amplitude range of 1.9-65 mT was applied to the platelets assigned to the channel where the magnetic stirring rod tip was used. Aggregation was induced using adenosine diphosphate (ADP), collagen, or epinephrine. The slopes, maximum aggregation values, and areas under the aggregation curves were compared between the magnetic and neutral stirring rod tip groups. For samples stirred with the magnetic stirring rod tip, a significant decrease was observed in 12 of the 14 parameters evaluated for aggregations induced with ADP or collagen compared to the neutral stirring rod tip, regardless of the method used for anticoagulation. This observation indicates that the magnetic stir bars used in standard aggregometry may significantly alter aggregation parameters and platelets may be possible targets of electromagnetic fields.

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Section: Discussionmentioning

confidence: 99%

“…With the help of this motor and magnet, the stir bar inside the PRP is spun, thus stirring the PRP sample (Fig. 1a from Sağdilek et al [2012]).…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Does the magnetic field of a magnetic stirrer in an optical aggregometer affect concurrent platelet aggregation?

Sağdilek

Sebik

Çelebi

2013

Bioelectromagnetics

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“…Explaining the aggregation pattern can be simple. Tiny molecules and proteins exist in blood and contribute in rouleaux formation [Iino, 1997 andSağdilek et al, 2012].…”

Section: Red Blood Cells Aggregationmentioning

confidence: 99%

“…Yet, the biological effect of non-ionization radiation is controversial [Ismail, H., 2015]. The effect of MF on living cells, red blood cells (RBCs) aggregations, rotations and magnetizations [Iino, 1997 andSağdilek et al, 2012], DNA single and double stand break [Ali, 2018 andMcCann, 1998], viscosity of blood [Kenjereš, 2008 andMohaseb et al, 2017], heating, body weight, oxidative stress, blood pressure, brain tissue, circulation system, blood proteins, PH and ect. were studied.…”

Section: Introductionmentioning

confidence: 99%

A review of the Effects of Magnetic Field on main blood cells: in vivo and in vitro experiments.

2019

ZJPAS

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Background: Magnetic field has being used in diagnostics for decades such as MRI. Sources of magnetic field were found in electronic devices and may emit extremely low intensity of magnetic field. Beside the daily use of electronic devices, there are some concerns regarding to side effects of magnetic field produces on living cells. Main Blood cells parameters were considered, and Red blood cells have targeted as the main and most affected by magnetic field. Concerns to blood, any abnormality in shape, aggregation, count may cause inflammations or chronic diseases such as anemia. Objective: In this review, we evaluated the previous works carried out to investigate the effect of static and time varying magnetic fields on rheological properties (blood parameters, viscosity and DNA strand break) of blood between periods from 1980-2019. We provided up-to-date state of research articles and the latest progress. Different intensities of magnetic fields (week, moderate and strong) were looked at. Result: Seventy two published research articles were reviewed. This shortened to thirty eight articles in respect to our goal. The chosen articles studied the biological effect of magnetic field on human and animal in vivo and in vitro experiments. A few theoretical studies were pointed to. Inconsistent results were compared. Conclusion: Magnetic fields can be static or time varying as frequency is not zero. Blood counts responded to external magnetic field with altering in counts, aggregations, change in viscosity and DNA strand break. RBCs aggregation increased as blood is affected by magnetic field. Whole blood viscosity enhanced the aggregation of red blood cells. Raise in aggregation changed the time flow of blood in microcirculations.

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Spectrophotometric Study of the Effect of a Magnetic Field on Human Blood Components

et al. 2016

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Investigation of the effects of 50 Hz magnetic fields on platelet aggregation using a modified aggregometer

Cited by 6 publications

References 26 publications

Does the magnetic field of a magnetic stirrer in an optical aggregometer affect concurrent platelet aggregation?

Does the magnetic field of a magnetic stirrer in an optical aggregometer affect concurrent platelet aggregation?

A review of the Effects of Magnetic Field on main blood cells: in vivo and in vitro experiments.

Spectrophotometric Study of the Effect of a Magnetic Field on Human Blood Components

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