Investigation of the faecal microbiota of kittens: monitoring bacterial succession and effect of diet

Jia, Jie; Frantz, N.Z.; Khoo, Christina; Gibson, Glenn R.; Rastall, Robert A.; McCartney, Anne L.

doi:10.1111/j.1574-6941.2011.01172.x

Cited by 26 publications

(31 citation statements)

References 38 publications

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“…For descriptive purposes, we included the results from the 2 foals from which we had data at other ages (these data were not included in the statistical analysis comparing ages). The resident intestinal or fecal microbiota has been described for neonates of other species, such as cats [41], [42], dogs [43], and humans [25]–[27], [44]. To the authors’ knowledge, this is the first report of age-related changes of the fecal microbiome in foals.…”

Section: Discussionmentioning

confidence: 81%

Effects of Administration of Live or Inactivated Virulent Rhodococccus equi and Age on the Fecal Microbiome of Neonatal Foals

et al. 2013

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Background Rhodococcus equi is an important pathogen of foals. Enteral administration of live, virulent R. equi during early life has been documented to protect against subsequent intrabronchial challenge with R. equi, indicating that enteral mucosal immunization may be protective. Evidence exists that mucosal immune responses develop against both live and inactivated micro-organisms. The extent to which live or inactivated R. equi might alter the intestinal microbiome of foals is unknown. This is an important question because the intestinal microbiome of neonates of other species is known to change over time and to influence host development. To our knowledge, changes in the intestinal microbiome of foals during early life have not been reported. Thus, the purpose of this study was to determine whether age (during the first month of life) or administration of either live virulent R. equi (at a dose reported to protect foals against subsequent intrabronchial challenge, viz., 1×1010 colony forming units [CFU]) or inactivated virulent R. equi (at higher doses, viz., 2×1010 and 1×1011 [CFU]) altered the fecal microbiome of foals.Methodology/Principal FindingsFecal swab samples from 42 healthy foals after vaccination with low-dose inactivated R. equi (n = 9), high-dose inactivated R. equi (n = 10), live R. equi (n = 6), control with cholera toxin B (CTB, n = 9), and control without CTB (n = 8) were evaluated by 454-pyrosequencing of the 16S rRNA gene and by qPCR. No impact of treatment was observed among vaccinated foals; however, marked and significant differences in microbial communities and diversity were observed between foals at 30 days of age relative to 2 days of age.ConclusionsThe results suggest age-related changes in the fecal microbial population of healthy foals do occur, however, mucosal vaccination does not result in major changes of the fecal microbiome in foals.

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Section: Discussionmentioning

confidence: 81%

Effects of Administration of Live or Inactivated Virulent Rhodococccus equi and Age on the Fecal Microbiome of Neonatal Foals

et al. 2013

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“…The microbiota of kittens have been described using DGGE (Denaturing Gradient Gel Electrophoresis) and this data suggests that microbial diversity and richness decreases from 4 weeks old through weaning [11]. A study in kittens using pyrosequencing where fecal samples were taken at 8, 12, and 16 weeks, however, show no significant changes in bacterial diversity among ages [12].…”

Section: Introductionmentioning

confidence: 99%

Characterization of the fecal microbiome during neonatal and early pediatric development in puppies

et al. 2017

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Limited information is available describing the development of the neonatal fecal microbiome in dogs. Feces from puppies were collected at 2, 21, 42, and 56 days after birth. Feces were also collected from the puppies’ mothers at a single time point within 24 hours after parturition. DNA was extracted from fecal samples and 454-pyrosequencing was used to profile 16S rRNA genes. Species richness continued to increase significantly from 2 days of age until 42 days of age in puppies. Furthermore, microbial communities clustered separately from each other at 2, 21, and 42 days of age. The microbial communities belonging to dams clustered separately from that of puppies at any given time point. Major phylogenetic changes were noted at all taxonomic levels with the most profound changes being a shift from primarily Firmicutes in puppies at 2 days of age to a co-dominance of Bacteroidetes, Fusobacteria, and Firmicutes by 21 days of age. Further studies are needed to elucidate the relationship between puppy microbiota development, physiological growth, neonatal survival, and morbidity.

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“…A recent study evaluated the development of the fecal microbiota of kittens from 4 weeks to 9 months of age, and observed higher species diversity but also a more variable microbial profile in 4-week-old kittens compared to weaned kittens at 8 weeks of age (Jia et al, 2011b). Furthermore, microbiota changes were also observed in relation to diet succession in these growing kittens (Jia et al, 2011b). In kittens 8, 12, and 16 weeks of age, Hooda et al (unpublished data) reported that Firmicutes was the predominant phylum (70-80% of sequences), followed by moderate Actinobacteria and Fusobacteria populations, and low Proteobacteria and Bacteroidetes populations.…”

Section: Development Of Gi Microbiotamentioning

confidence: 99%

Feline gastrointestinal microbiota

Minamoto

Hooda

Swanson

et al. 2012

Anim. Health. Res. Rev.

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AbstractThe close relationship between gastrointestinal (GI) microbiota and its host has an impact on the health status of an animal that reaches beyond the GI tract. A balanced microbiome stimulates the immune system, aids in the competitive exclusion of transient pathogens and provides nutritional benefits to the host. With recent rapid advances in high-throughput sequencing technology, molecular approaches have become the routinely used tools for ecological studies of the feline microbiome, and have revealed a highly diverse and complex intestinal ecosystem in the feline GI tract. The major bacterial groups are similar to those found in other mammals, withFirmicutes,Bacteroidetes,ActinobacteriaandProteobacteriaconstituting more than 99% of intestinal microbiota. Several nutritional studies have demonstrated that the feline microbiota can be modulated by the amount of soluble fibers (i.e., prebiotics) and macronutrients (i.e., protein content) in the diet. Initial clinical studies have suggested the presence of a dysbiosis in feline inflammatory bowel disease (IBD). Recently, metagenomic approaches have attempted to characterize the microbial gene pool. However, more studies are needed to describe the phylogenetic and functional changes in the intestinal microbiome in disease states and in response to environmental and dietary modulations. This paper reviews recent studies cataloging the microbial phylotypes in the GI tract of cats.

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Investigation of the faecal microbiota of kittens: monitoring bacterial succession and effect of diet

Cited by 26 publications

References 38 publications

Effects of Administration of Live or Inactivated Virulent Rhodococccus equi and Age on the Fecal Microbiome of Neonatal Foals

Effects of Administration of Live or Inactivated Virulent Rhodococccus equi and Age on the Fecal Microbiome of Neonatal Foals

Characterization of the fecal microbiome during neonatal and early pediatric development in puppies

Feline gastrointestinal microbiota

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