Investigation of the genetic variation of anise (Pimpinella anisum L.) using RAPD and ISSR markers

Giachino, R. Refika Akçali

doi:10.1007/s10722-019-00861-y

Cited by 26 publications

(13 citation statements)

References 48 publications

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“…Previous work for other plant species has reported similar results for EMR and IM settings with small differences (Giachino (2019); Mukherjee et al (2013), Kumar et al (2014) and Tagizad et al (2010)).…”

Section: Genotypes Characterizationsupporting

confidence: 69%

Molecular Diversity of Walnut (Juglans regia L.) Among Two Major Areas in Morocco in Contrast with Foreign Varieties

Houmanat

Kajji

Hssaini

et al. 2020

International Journal of Fruit Science

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In Morocco, walnut tree (Juglans regia L.) cultivation is ancestral with complicated misidentification and unknown local genetic diversity. Atlas Mountains host a rich walnut tree germplasm, which has arisen from seedlings. The use of molecular markers makes it possible to overcomes confusion usually encountered in phenotypic characterization, thereby elucidating the real genetic diversity magnitude. This study aimed to investigate the genetic relationships among 33 local walnut trees grown in two contrasted agroecosystems, while comparing them with 8 Bulgarian varieties belonging to an ex-situ collection, using ISSR markers. The use of 13 primers generated 120 reproducible ISSR markers, with 7 to 13 bands and an average of nine bands per primer. This number reflected the high level of polymorphism between genotypes revealed by the selected primers. The average polymorphism rate was 75.2%, with the polymorphic information content varying from 0.212 to 0.370 and with an average of 0.32. Genetic distances and the pairwise comparison showed that all pairs of genotypes are distinct using more than 10 markers with a maximum of 50 bands. The dendrogram showed an obvious phylo-geographic structuring and the differentiation of genotypes by origin area. The Bulgarian cultivars form distinct groups that confirms the specificity of the Moroccan walnut gene pool. However, the dendrogram obtained showed that the Moroccan genotypes are genetically close to a certain number of Bulgarian genotypes. This indicates that some Moroccan genotypes and the Bulgarian varieties could have a common ancestor.

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Section: Genotypes Characterizationsupporting

confidence: 69%

Molecular Diversity of Walnut (Juglans regia L.) Among Two Major Areas in Morocco in Contrast with Foreign Varieties

Houmanat

Kajji

Hssaini

et al. 2020

International Journal of Fruit Science

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“…The polymorphic information content value provides the discriminating power of the locus using expressed number of alleles and the frequency of those alleles [27] being an important tool in representing the genetic diversity. Accordingly, the maximum PIC value for a dominant marker is 0.5 [28] and the PIC value generated in the present study by primer UBC 840a was 0.401. selected to analyze genetic variation between A. cherimola, A. squamosa and their hybrids where a total of 92 bands were produced while 86 bands (93.5 percent) were polymorphic [24].…”

Section: Discussionmentioning

confidence: 99%

“…According to the dendrogram, fifty soursop genotypes were separated into four major clusters at 80% genetic similarity level (Fig. 3) Cluster three included fourteen genotypes collected from ARS, Girandurukotte (27,28,29,30,31,32,33,34,35,36,37,38,39,40) grouped according to the geographical origin of the genotypes. The cluster provided evidence for the close proximity and similar morphology (leaf type, fruit size, pulp colour, brix value) of the genotypes within the same geographical location sharing a common origin.…”

Section: -Gk-175 28-gk-159 29-gk-170 30-gk-197 31-gk-90 32-gk-mentioning

confidence: 99%

“…The cluster provided evidence for the close proximity and similar morphology (leaf type, fruit size, pulp colour, brix value) of the genotypes within the same geographical location sharing a common origin. Cluster four composed of genotypes collected from IFCO Farm, Badulla (11,12,13,14,15,16,17,18) and AF, HORDI (19,20) (11),RW2898 (12),RW2918 (13),RW2921 (14),RW654 (15),SSV659 (16),SSV640 (17),SSV649 (18),AF,HORDI-Am 01 (19),Am 02 (20) (27), , , , , ,GK-172 (38), , , ,GK-30 (39),GK-25 (35)}…”

Section: -Gk-175 28-gk-159 29-gk-170 30-gk-197 31-gk-90 32-gk-mentioning

confidence: 99%

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Genetic Diversity Assessment of Selected Annona muricata L. Germplasm in Sri Lanka

Samaradiwakara

Samarasinghe

Shantha³

et al. 2020

AJAHR

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Annona muricata L. commonly known as soursop is an underutilized fruit crop species in Sri Lanka gaining much importance in the recent past due to its high nutritional and medicinal value. Soursop germplasm collections are available within the country and assessing the genetic diversity is needed to proceed with conservation, detecting promising lines and breeding programs. This study was conducted to assess the genetic diversity of 50 soursop individuals using Inter-Simple Sequence Repeat (ISSR) markers. The study was conducted at Plant Genetic Resources Centre of the Department of Agriculture in Gannoruwa during 2017 to 2019. DNA of the 50 soursop samples were extracted using CTAB method and Polymerase Chain Reaction (PCR) was carried using 13 Inter Simple Sequence Repeat (ISSR) Markers. PCR products were visualized using 1.5 percent Agarose gel electrophoresis under the Biorad Gel documentation system and analyzed using POPGENE 1.31. PCR amplified 139 bands from 13 ISSR markers among which 118 were found to be polymorphic. The polymorphic band percentage was 85 percent while as the average number of bands observed (Na) was 1.8489 and the effective allele number (Ne) was 1.5377. The Nei's gene diversity index (h) was 0.3079. The Shannon Information Index (I) found to be 0.4556. Dendrogram constructed based on the UPGMA method clustered the studied accessions into four major clusters at 80 percent similarity level. Results revealed considerable degree of genetic diversity existed within the studied soursop germplasms at Sri Lanka. Existing genetic diversity within soursop individuals will serve as germplasm bank to identify and utilize potential germplasm resources for conservation and future breeding programs to develop quality soursop varieties in Sri Lanka.

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“…They have been utilized in the investigation of genetic variation between closely related individuals and identification in various species (Zietkiewicz et al, 1994). ISSR markers have been also successfully employed to determine relationships at the population and species levels and a variety of plant species, including various aromatic and medicinal plants; e.g., Pimpinella anisum (Akcali Giachino, 2019), Achillea millefolium , Artemisia capillaries (Shafie et al, 2009), Thymus daenensis (Rahimmalek et al, 2009), safflower (Yang et al 2007), and Helichrysum Mill. species (Azizi et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Bazı rezene popülasyonlarının (Foeniculum vulgare Mill.) ISSR markörlerine dayalı moleküler varyasyonu

Giachino¹,

Avcı²

2020

Anadolu Journal of Agricultural Sciences

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This research was conducted to determine the molecular variation between different fennel populations. In this research, six fennel populations, of which two were collected from natural flora, were used as a material. The molecular characterization of the populations was performed using six ISSR (Inter simple sequence repeat) primers. A total of 57 bands were obtained, of which 48 were evaluated as polymorphic. The average polymorphism percentage was 85%. The PIC values changed from 0.33 (ISSR-16) to 0.43 (ISSR-11) with an average of 0.37. The average RP (Resolving Power) value of the primers was found as 4.17, and the highest value was obtained from the ISSR-4 (6.33) primer. The mean EMR value was 6.78, and the highest value was obtained from the ISSR-4 (10.29) primer. The mean MI (Marker Index) value was estimated to be 2.47, with the highest value similarly observed in the ISSR-4 (3.67) primer. ISSR-4 was the most prominent primer in terms of these three parameters and the maximum total and polymorphic bands. The similarity values of the fennel populations were found to be between 0.30 and 0.95. The Mental test conducted between Jaccard's similarity matrices and the cluster result revealed the r value as 0.99, showing a very good correlation between the ISSR-based similarity matrix and the cluster. The populations of Burdur, Denizli, Isparta and Izmir, which were cultivated and traded, were genetically similar in terms of the ISSR primers examined, whereas Manisa Yunt Mountain and Odemis populations collected from natural flora were in separate groups.

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Investigation of the genetic variation of anise (Pimpinella anisum L.) using RAPD and ISSR markers

Cited by 26 publications

References 48 publications

Molecular Diversity of Walnut (Juglans regia L.) Among Two Major Areas in Morocco in Contrast with Foreign Varieties

Molecular Diversity of Walnut (Juglans regia L.) Among Two Major Areas in Morocco in Contrast with Foreign Varieties

Genetic Diversity Assessment of Selected Annona muricata L. Germplasm in Sri Lanka

Bazı rezene popülasyonlarının (Foeniculum vulgare Mill.) ISSR markörlerine dayalı moleküler varyasyonu

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