The major aim of the research is to investigate the ability of biogas as an alternative fuel for gasoline-powered Spark Ignition (SI) engine. In this study, biogas/gasoline fuel mixtures containing different ratios of biogas, gasoline, and biogas were tested in an SI engine with an increased compression ratio at different engine loads and constant engine speed. According to the comparison with gasoline, the utilization of biogas generally decreased the Brake Thermal Efficiency (BTE), while the Brake Specific Fuel Consumption (BSFC) rose. The lowest BTE and the highest BSFC were obtained with 100% biogas. Compared to gasoline, a decrease of 16.04% and an increase of 75.52% were observed, respectively. On the other hand, the use of biogas has improved all emissions. The best emission values were obtained with 100% biogas. Compared to gasoline, Carbon monoxide (CO), HydroCarbon (HC), and Nitrogen Oxide (NOx) emissions decreased by 56.42%, 63%, and 48.96%, respectively. Finally, according to the results of the combustion analysis, the peak pressures were reduced with the utilization of biogas, and the position of the peak pressure shifted by 2° to 3° Crank Angle (CA). Compared to gasoline, the lowest pressure was obtained with 100% biogas, resulting in a reduction of approximately 24.69%.