Investigation of the in vitro cytotoxic effects and wound healing activity of ternary composite substance (hollow silica sphere/gum arabic/methylene blue)

Duran, Hakan; Alkan, Fulya Üstün; Ulkay, M. Başak; Karakuş, Selcan; Aktaş, Abit; Şişmanoğlu, Tuba

doi:10.1016/j.ijbiomac.2018.10.098

Cited by 14 publications

(5 citation statements)

References 20 publications

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“…Upon reaching 70-90% confluence, cells were detached using 0.25% trypsin-EDTA and collected to be plated. Toxicological studies followed the methodology described by Duran et al [48]. Briefly, batches of 5000 HDF cells/well or 20,000 CPEK cells/well were plated and allowed to sediment overnight.…”

Section: Toxicological Assessmentmentioning

confidence: 99%

Antimicrobial, Antibiofilm and Toxicological Assessment of Propolis

et al. 2023

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Antimicrobial resistance is a serious problem for the control of infections and infectious diseases. Propolis is a substance produced by honeybees with antimicrobial and antibiofilm properties. To consider propolis as an alternative to the use of antimicrobials for infection control, we assessed its antimicrobial and antibiofilm activities. To assess propolis for topical medical use, toxicological studies were also performed. A Portuguese 70% propolis ethanolic extract was chemically evaluated and studied for antimicrobial activity on staphylococcal field isolates (n = 137) and antibiofilm action (n = 45). Cell toxicological assessment was performed using keratinocytes and fibroblasts. Pinobanksin, chrysin, acacetin, apigenin, pinocembrin, and kaempferol-dimethyl-ether were detected. All 137 isolates were susceptible to 6.68 mg/mL or lower propolis concentration (80% isolates were susceptible to <1 mg/mL). The mean percentage of biofilm inhibition was 71%, and biofilm disruption was 88.5%. Propolis (<1 mg/mL) was well-tolerated by fibroblasts and moderately tolerated by keratinocytes. The combined antimicrobial and antibiofilm effect of propolis, together with its low toxicity to connective tissue and epithelial cells, suggests a good applicability for topical antibacterial treatment. Therefore, propolis seems to be a good alternative to antimicrobials for the treatment of infections with Staphylococcus spp. that deserves to be evaluated in vivo.

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Section: Toxicological Assessmentmentioning

confidence: 99%

Antimicrobial, Antibiofilm and Toxicological Assessment of Propolis

et al. 2023

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“…Adsorption studies of MB used different concentrations ranging from 5 to 50 mg/mL onto 0.01 g adsorbent at 35 • C. At concentrations of 10, 50, and 100 µg/mL no cytotoxicity was observed after 72 h together with a moderate migration activity in a concentration-dependent manner. MB seems to improve scratch closure between 13.23% and 54.81% compared to negative control after 72 h of wounding [67].…”

Section: Methylene Blue Uses For In Vitro Studiesmentioning

confidence: 88%

Comparative Study Regarding the Properties of Methylene Blue and Proflavine and Their Optimal Concentrations for In Vitro and In Vivo Applications

et al. 2020

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Methylene blue and proflavine are fluorescent dyes used to stain nucleic acid from the molecular level to the tissue level. Already clinically used for sentinel node mapping, detection of neuroendocrine tumors, methemoglobinemia, septic shock, ifosfamide-induced encephalopathy, and photodynamic inactivation of RNA viruses, the antimicrobial, anti-inflammatory, and antioxidant effect of methylene blue has been demonstrated in different in vitro and in vivo studies. Proflavine was used as a disinfectant and bacteriostatic agent against many gram-positive bacteria, as well as a urinary antiseptic involved in highlighting cell nuclei. At the tissue level, the anti-inflammatory effects of methylene blue protect against pulmonary, renal, cardiac, pancreatic, ischemic-reperfusion lesions, and fevers. First used for their antiseptic and antiviral activity, respectively, methylene blue and proflavine turned out to be excellent dyes for diagnostic and treatment purposes. In vitro and in vivo studies demonstrated that both dyes are efficient as perfusion and tissue tracers and permitted to evaluate the minimal efficient concentration in different species, as well as their pharmacokinetics and toxicity. This review aims to identify the optimal concentrations of methylene blue and proflavine that can be used for in vivo experiments to highlight the vascularization of the skin in the case of a perforasome (both as a tissue tracer and in vascular mapping), as well as their effects on tissues. This review is intended to be a comparative and critical presentation of the possible applications of methylene blue (MB) and proflavine (PRO) in the surgical field, and the relevant biomedical findings from specialized literature to date are discussed as well.

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“…Our cytotoxicity assessment was carried out on murine macrophage (RAW 264.7) and fibroblast (3T3 Swiss Albino) cell lines (Manassass, Virginia, USA). Although immortalized by viral vectors, these cells retain many physiological features and are considered to be proper models of normal cells [42,43]. The choice of these two models was dictated by the possible biomedical application of the biomaterials under study as the implant covering surface.…”

Section: Cell Lines and Culturementioning

confidence: 99%

Cytotoxicity Study of UV-Laser-Irradiated PLLA Surfaces Subjected to Bio-Ceramisation: A New Way towards Implant Surface Modification

Szustakiewicz

Kryszak

Dzienny

et al. 2021

IJMS

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In this research we subjected samples of poly(L-lactide) (PLLA) extruded film to ultraviolet (193 nm ArF excimer laser) radiation below the ablation threshold. The modified film was immersed in Simulated Body Fluid (SBF) at 37 °C for 1 day or 7 days to obtain a layer of apatite ceramic (CaP) coating on the modified PLLA surface. The samples were characterized by means of optical profilometry, which indicated an increase in average roughness (Ra) from 25 nm for the unmodified PLLA to over 580 nm for irradiated PLLA incubated in SBF for 1 day. At the same time, the water contact angle decreased from 78° for neat PLLA to 35° for irradiated PLLA incubated in SBF, which suggests its higher hydrophilicity. The obtained materials were investigated by means of cell response fibroblasts (3T3) and macrophage-like cells (RAW 264.7). Properties of the obtained composites were compared to the unmodified PLLA film as well as to the UV-laser irradiated PLLA. The activation of the PLLA surface by laser irradiation led to a distinct increase in cytotoxicity, while the treatment with SBF and the deposition of apatite ceramic had only a limited preventive effect on this harmful impact and depended on the cell type. Fibroblasts were found to have good tolerance for the irradiated and ceramic-covered PLLA, but macrophages seem to interact with the substrate leading to the release of cytotoxic products.

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Investigation of the in vitro cytotoxic effects and wound healing activity of ternary composite substance (hollow silica sphere/gum arabic/methylene blue)

Cited by 14 publications

References 20 publications

Antimicrobial, Antibiofilm and Toxicological Assessment of Propolis

Antimicrobial, Antibiofilm and Toxicological Assessment of Propolis

Comparative Study Regarding the Properties of Methylene Blue and Proflavine and Their Optimal Concentrations for In Vitro and In Vivo Applications

Cytotoxicity Study of UV-Laser-Irradiated PLLA Surfaces Subjected to Bio-Ceramisation: A New Way towards Implant Surface Modification

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