Investigation of the in Vitro Regeneration of Some Medical and Aromatic Wild Plant Species

Tunçer, Burcu

doi:10.15666/aeer/1504_905914

Cited by 9 publications

(11 citation statements)

References 12 publications

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“…in the traditional medicine of several countries worldwide together with the increased interest in the protection of endangered species. Single node explants from F. orientalis L. were studied by Tuncer (2017) and shoots induction was obtained by culturing in Murashige/Skoog (MS) medium with the addition of 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzylaminopurine (BAP) (0.5 and 2.0 mg/L, respectively) as plant growth regulators. With this method, the production of three shoots was obtained for each explants, thus resulting to be a useful in vitro regeneration method.…”

Section: Propagation Of Ferula Speciesmentioning

confidence: 99%

The genus Ferula: Ethnobotany, phytochemistry and bioactivities – A review

Mohammadhosseini

Venditti

Sarker

et al. 2019

Industrial Crops and Products

129

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This study aims to provide a comprehensive overview of the medicinal, folkloric and traditional culinary uses of Ferula species, related products and extracts in different countries together with the description of recently isolated new components and the related bioactivities. The phytochemical composition of the essential oils (EOs), oleo-gum-resin (OGR) and the non-volatile fractions obtained from several endemic and indigenous Ferula species is also reported. A special emphasis is placed on their unusual components, i.e. sulfur-containing volatiles from the EOs and the new phytochemicals with mixed biogenetic origins. More than 180 chemical constituents (excluding common essential oils components), including sulfur-containing metabolites, terpenoids, coumarins, sesquiterpene coumarins, etc., as both aglycones and glycosides, are reported, along with their occurrence and biological activities when available. A large number of new secondary metabolites, belonging to different classes of natural products possessing interesting biological activities, from the antiproliferative to the anti-inflammatory to the neuroprotective ones, among the others, have been recently found in the Ferula genus. Several of these phytochemicals are exclusive to this genus; therefore may be considered chemotaxonomic markers. All these aspects are extensively discussed in this review.

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Section: Propagation Of Ferula Speciesmentioning

confidence: 99%

The genus Ferula: Ethnobotany, phytochemistry and bioactivities – A review

Mohammadhosseini

Venditti

Sarker

et al. 2019

Industrial Crops and Products

129

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“…In vitro mass micropropagation techniques have been considered a promising procedure for the multiplication of valuable, hard-to-propagate and endangered wild species [12]. Although in vitro regeneration studies have been performed on many genera from the Liliaceae family, such as Aloe, Lilium, Chlorophytum, Feritillaria, and Scilla [13][14][15][16][17][18][19][20], only a few studies have reported on the in vitro regeneration of E. spectabilis worldwide [21,22]. In a recent article [21], the leaf and rhizome explants of E. spectabilis were cultured on Murashige and Skoog (MS) media [23], supplemented with 6-benzylaminopurine (BAP) and 2, 4-dichlorophenoxyacetic acid (2, 4-D).…”

Section: Introductionmentioning

confidence: 99%

“…Although in vitro regeneration studies have been performed on many genera from the Liliaceae family, such as Aloe, Lilium, Chlorophytum, Feritillaria, and Scilla [13][14][15][16][17][18][19][20], only a few studies have reported on the in vitro regeneration of E. spectabilis worldwide [21,22]. In a recent article [21], the leaf and rhizome explants of E. spectabilis were cultured on Murashige and Skoog (MS) media [23], supplemented with 6-benzylaminopurine (BAP) and 2, 4-dichlorophenoxyacetic acid (2, 4-D). However, the results showed no in vitro shoot regeneration regardless of explant types.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Culture of Eremurus spectabilis (Liliaceae), a Rare Ornamental and Medicinal Plant, through Root Explants

et al. 2022

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Eremurus spectabilis M. Bieb, a perennial herbaceous wild species, is commonly used in the horticultural, ornamental, and pharmaceutical markets. Studies on the tissue culture systems for this species would be beneficial for mass multiplication as well as for future breeding programs. An in vitro propagation technique was established here using tuberous root explants as unique and responsive starting materials for culture initiation. The proliferated calli were sub-cultured on shoot proliferation media and regenerated microshoots were assessed. The shoot proliferation rate, leaf number, leaf length, and chlorophyll and carotenoid contents were recorded. The highest callus induction per explant (76.67%), callus dry weight (10.25 mg), callus firmness ratio (3.97), and callus color intensity ratio (2.83) were observed in explants inoculated on Murashige and Skoog (MS) medium supplemented with 10.0 mgL−1 6-benzylaminopurine (BAP). The highest shoot proliferation rates were obtained when calli were sub-cultured on MS or Schenk and Hildebrandt (SH) basal media supplemented with 2.0 mgL−1 BAP. The half-strength MS medium fortified with 4.0% sucrose + 2.0 mgL−1 indole butyric acid (IBA) + 200 mgL−1 activated charcoal was a superior combination for root emergence and rooting parameters. Regenerated plantlets were then successfully adapted to ex vitro conditions. The reported protocol can be exploited at a commercial scale following minor modification, or could be beneficial in the production of secondary metabolites in bioreactors where callus is required as fresh plant material.

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“…Tissue culture method have been used to propagate and conserve several Himalayan medicinal plants, Bunium persicum, Wakhlu et al (1990); Heracleum candicans, Wakhlu and Sharma (1998); Podophyllum hexandrum, Rajesh et al (2014); Sapindus mukorossi, Singh et al (2015) and Viola canescens, Khajuria et al (2019). Micropropagation studies have been reported for number of Ferula spp i.e., F. gummosa, Bernard et al (2007), Hadi et al (2011a, b); F. assafoetida, Hasani et al (2008), Zare et al (2010), Otroshy et al (2013) and Otroshy and Roozbeh (2015); F. orientalis, Tuncer (2017); F. ferulaeoides, Suran et al (2016). Off the all reported documents no in vitro studies have been reported for best to our knowledge on F. jaeschkeana, except our early work i.e., callus induction studies F. jaeschkeana, Sharma (2015).…”

Section: Introductionmentioning

confidence: 99%

Somatic embryogenesis and plant regeneration in Ferula jaeschkeana Vatke: a threatened medicinal herb

Sharma

Khajuria

2020

Vegetos

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An efficient in vitro propagation protocol for an important medicinal endangered herb Ferula jaeschkeana has been developed through somatic embryogenesis. 12 week old friable calli were derived from petiole explants on a medium supplemented with 2.0 mgl −1 2,4-D and 1.0 mgl −1 Kn and used as mother source for somatic embryogenesis. Different auxins (2,4-D, IBA, IAA and NAA) in different concentrations (0.2-4.0 mgl −1 ) was used to produce embryogenic callus. Eighty four percent calli produced globular embryos on a medium supplemented with 4.0 mgl −1 2,4-D. The globular embryo then mature on culture medium (Control) or supplemented with different addictives (auxin, proline and ABA), maturation was significantly affected by ABA, proline and number of subcultures with maximum number of mature embryo (32.1 ± 0.2) was formed on medium fortified with 2.0 mgl −1 proline. Mature embryos had two well-developed cotyledons and an elongated hypocotyl root axis. 78% of these embryos were transformed into plantlets when placed in culture medium fortified with 0.2 + 0.01 + 0.05 mgl −1 (BAP:IBA:GA 3 ) respectively. Regenerated plants were hardened in vermiculite with a 23% survival rate. The regenerated plants showed normal diploid chromosome number (2n = 22) and leaf morphology was comparable to in vivo plant.

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Investigation of the in Vitro Regeneration of Some Medical and Aromatic Wild Plant Species

Abstract: Tuncer: Investigation of the in vitro regeneration of some medical and aromatic wild plant species -

Cited by 9 publications

References 12 publications

The genus Ferula: Ethnobotany, phytochemistry and bioactivities – A review

The genus Ferula: Ethnobotany, phytochemistry and bioactivities – A review

In Vitro Culture of Eremurus spectabilis (Liliaceae), a Rare Ornamental and Medicinal Plant, through Root Explants

Somatic embryogenesis and plant regeneration in Ferula jaeschkeana Vatke: a threatened medicinal herb

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