Investigation of the intestinal trans-epithelial transport and antioxidant activity of two hempseed peptides WVSPLAGRT (H2) and IGFLIIWV (H3)

Bollati, Carlotta; Cruz‐Chamorro, Ivan; Aiello, Gilda; Li, Jianqiang; Bartolomei, Martina; Santos-Sánchez, Guillermo; Ranaldi, Giulia; Ferruzza, Simonetta; Sambuy, Yula; Arnoldi, Anna; Lammi, Carmen

doi:10.1016/j.foodres.2021.110720

Cited by 32 publications

(45 citation statements)

References 36 publications

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“…Recently, we demonstrated that peptides H2 and H3 exert antioxidant activity in HepG2 cells, modulating both the Nrf-2 and iNOS pathways, which led to the reduction of cellular H 2 O 2 -induced ROS, NO, and lipid peroxidation levels. 22 Since an increase in oxidative stress is always accompanied by an inflammatory process, it was interesting to study the immunomodulatory capacity of these two hempseed peptides in the same cellular system. Notably, HepG2 cells have been widely used as a model for characterizing the anti-inflammatory activity of many food-active compounds.…”

Section: Discussionmentioning

confidence: 99%

“… 25 − 28 To achieve this objective, we decided to perform tests under the same conditions and study the same concentration of each peptide H2 (100 μM) and H3 (25 μM), which was previously demonstrated to be safe from a cytotoxic point of view and also effective for antioxidant activity. 22 In particular, HepG2 cells were stimulated with LPS, a generic and commonly used pro-inflammatory stimulus. Therefore, the LPS stimulation activates the NF-κB and iNOS pathways.…”

Section: Discussionmentioning

confidence: 99%

“…A very interesting feature of both H2 and H3 is that despite IRW and IQW, they are transported by intestinal cells and they are stable toward intestinal protease activity when they are within the hempseed hydrolysate. 22 …”

Section: Discussionmentioning

confidence: 99%

“…In addition, although many food protein hydrolysates have demonstrated anti-inflammatory effects, 43 our study is the pioneer in the identification of anti-inflammatory peptides that can be absorbed by the human intestinal barrier from the hempseed source. 22 …”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, H2 and H3 modulate the nuclear factor erythroid 2-related factor 2 (Nrf-2) and inducible nitric oxide synthase (iNOS) pathways in H 2 O 2 -stimulated HepG2 cells. 22 …”

Section: Introductionmentioning

confidence: 99%

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Hempseed (Cannabis sativa) Peptides WVSPLAGRT and IGFLIIWV Exert Anti-inflammatory Activity in the LPS-Stimulated Human Hepatic Cell Line

Cruz‐Chamorro

Santos-Sánchez

Bollati

et al. 2022

J. Agric. Food Chem.

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WVSPLAGRT (H2) and IGFLIIWV (H3) are two transepithelial transported intestinal peptides obtained from the hydrolysis of hempseed protein with pepsin, which exert antioxidant activity in HepG2 cells. Notably, both peptides reduce the H 2 O 2 -induced reactive oxygen species, lipid peroxidation, and nitric oxide (NO) production levels in HepG2 cells via the modulation of the nuclear factor erythroid 2-related factor 2 and the inducible nitric oxide synthase (iNOS) pathways, respectively. Due to the close link between inflammation and oxidative stress and with the objective of fostering the multifunctional behavior of bioactive peptides, in this study, the molecular characterization of the anti-inflammatory and immunomodulatory properties of H2 and H3 was carried out in HepG2 cells. In fact, both peptides were shown to modulate the production of pro (IFN-γ: −33.0 ± 6.7% H2, p = 0.011; −13.1 ± 2.0% H3, p = <0.0001; TNF: −17.6 ± 1.7% H2, p = 0.0004; −20.3 ± 1.7% H3, p = <0.0001; and IL-6: −15.1 ± 6.5% H3, p = 0.010)- and anti (IL-10: +9.6 ± 3.1% H2, p = 0.010; +26.0 ± 2.3% H3, p = < 0.0001)-inflammatory cytokines and NO (−9.0 ± 0.7% H2, p = <0.0001; −7.2 ± 1.8% H3, p = <0.0001) through regulation of the NF-κB and iNOS pathways, respectively, in HepG2 cells stimulated by lipopolysaccharides.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…Furthermore, H2 and H3 modulate the nuclear factor erythroid 2-related factor 2 (Nrf-2) and inducible nitric oxide synthase (iNOS) pathways in H 2 O 2 -stimulated HepG2 cells. 22 …”

Section: Introductionmentioning

confidence: 99%

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Hempseed (Cannabis sativa) Peptides WVSPLAGRT and IGFLIIWV Exert Anti-inflammatory Activity in the LPS-Stimulated Human Hepatic Cell Line

Cruz‐Chamorro

Santos-Sánchez

Bollati

et al. 2022

J. Agric. Food Chem.

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Plant‐derived peptides for the improvement of Alzheimer's disease: Production, functions, and mechanisms

Lin

Liu

et al. 2023

Food Frontiers

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Alzheimer's disease (AD) is a progressive neurodegenerative disease with an insidious onset that seriously affects human health. Plant-derived peptides have been found to hinder the development of AD pathologies, which is an excellent candidate for preventing AD. However, due to the excessively complicated pathogenesis of AD and the fact that most studies on the activity of plant-derived peptides are single and not deep enough, which restricted the development and application of plant-derived AD-prevention peptides (PADPs). This review summarized the currently available means of obtaining PADPs, in vitro and in vivo AD-prevention activity validation protocols, molecular pathways of PADPs, the structure-activity relationship between plant-derived peptides and AD-prevention activity, and some perspectives of current advanced technologies. This paper will help to develop the foundation for the production and exploitation of PADPs.

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Octopus‐derived antioxidant peptide protects against hydrogen peroxide‐induced oxidative stress in IEC‐6 cells

Peng

Cai

Pan

2022

Food Science & Nutrition

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This study aims to find antioxidant peptides from octopus protein hydrolyzates and verify the protective effects against H2O2‐induced oxidative stress in IEC‐6 cells. After the alcalase hydrolysate was ultrafiltrated, purified by Sephadex G‐25 gel fractionation and semipreparative reversed‐phase high‐performance liquid chromatography (RP‐HPLC), 16 peptides were identified, and chemically synthesized. In particular, the peptides AQNY, AMMLAW, FEGAW, GGAW, VDTVVCVW, and VVCLW showed better oxygen radical absorbance capacity (ORAC) and ABTS radical scavenging capacity. Among them, the smallest‐molecular‐weight peptide GGAW exhibited the best antioxidant activity. Furthermore, GGAW protected IEC‐6 cells from H2O2‐induced oxidative damage by significantly reducing the generation of reactive oxygen species (ROS), malondialdehyde (MDA), and lactate dehydrogenase (LDH), and increasing the activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH‐PX), thereby improving cell viability. These results indicated that the peptide GGAW possessed the antioxidant capacity to prevent oxidative stress damage.

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Investigation of the intestinal trans-epithelial transport and antioxidant activity of two hempseed peptides WVSPLAGRT (H2) and IGFLIIWV (H3)

Cited by 32 publications

References 36 publications

Hempseed (Cannabis sativa) Peptides WVSPLAGRT and IGFLIIWV Exert Anti-inflammatory Activity in the LPS-Stimulated Human Hepatic Cell Line

Hempseed (Cannabis sativa) Peptides WVSPLAGRT and IGFLIIWV Exert Anti-inflammatory Activity in the LPS-Stimulated Human Hepatic Cell Line

Plant‐derived peptides for the improvement of Alzheimer's disease: Production, functions, and mechanisms

Octopus‐derived antioxidant peptide protects against hydrogen peroxide‐induced oxidative stress in IEC‐6 cells

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