Investigation of the key factors on 3-hydroxypropionic acid production with different recombinant strains

Niu, Kun; Cheng, Xu; Qin, Hai-Bin; Liu, Ji-Song; Zheng, Yu‐Guo

doi:10.1007/s13205-017-0966-4

Cited by 6 publications

(10 citation statements)

References 31 publications

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“…As has been discussed, glycerol dehydratase undergoes rapid inactivation, which can result in shutdown of 3HP production. Niu et al [31] demonstrated that when the glycerol dehydratase reactivase gene was cloned together with the glycerol dehydratase gene, the 3HP concentration increased fivefold, due to the prevention of glycerol dehydratase inactivation.…”

Section: Engineering Of E Coli Cells For the Conversion Of Glycerol mentioning

confidence: 99%

Biological Production of 3-Hydroxypropionic Acid: An Update on the Current Status

et al. 2018

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Abstract:The production of high added-value chemicals from renewable resources is a necessity in our attempts to switch to a more sustainable society. 3-Hydroxypropionic acid (3HP) is a promising molecule that can be used for the production of an important array of high added-value chemicals, such as 1,3-propanediol, acrylic acid, acrylamide, and bioplastics. Biological production of 3HP has been studied extensively, mainly from glycerol and glucose, which are both renewable resources. To enable conversion of these carbon sources to 3HP, extensive work has been performed to identify appropriate biochemical pathways and the enzymes that are involved in them. Novel enzymes have also been identified and expressed in host microorganisms to improve the production yields of 3HP. Various process configurations have also been proposed, resulting in improved conversion yields. The intense research efforts have resulted in the production of as much as 83.8 g/L 3HP from renewable carbon resources, and a system whereby 3-hydroxypropionitrile was converted to 3HP through whole-cell catalysis which resulted in 184.7 g/L 3HP. Although there are still challenges and difficulties that need to be addressed, the research results from the past four years have been an important step towards biological production of 3HP at the industrial level.

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Section: Engineering Of E Coli Cells For the Conversion Of Glycerol mentioning

confidence: 99%

Biological Production of 3-Hydroxypropionic Acid: An Update on the Current Status

et al. 2018

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“…shown that the expression of GdrAB successfully restored the stability of DhaB (Niu et al, 2017;Rathnasingh et al, 2009).…”

Section: Supply Of Vb 12mentioning

confidence: 95%

“…The recombinant E. coli strain, containing the mutant GabD4_E209Q/E269Q which was achieved by site-directed and saturation mutagenesis based on homology modeling, produced 71.9g/L 3-HP (Chu et al, 2015). K. KGSADH (Niu et al, 2017). Although balancing two enzymes has been achieved using different plasmids in the above studies, it may not be suitable for industrial application because of plasmid instability during fermentation (Jers et al, 2019).…”

Section: Balance Between Dhab and Aldhmentioning

confidence: 99%

Recent advances, challenges and metabolic engineering strategies in the biosynthesis of 3‐hydroxypropionic acid

Liang

Sun

Nie

et al. 2022

Biotech & Bioengineering

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As an attractive and valuable platform chemical, 3‐hydroxypropionic acid (3‐HP) can be used to produce a variety of industrially important commodity chemicals and biodegradable polymers. Moreover, the biosynthesis of 3‐HP has drawn much attention in recent years due to its sustainability and environmental friendliness. Here, we focus on recent advances, challenges, and metabolic engineering strategies in the biosynthesis of 3‐HP. While glucose and glycerol are major carbon sources for its production of 3‐HP via microbial fermentation, other carbon sources have also been explored. To increase yield and titer, synthetic biology and metabolic engineering strategies have been explored, including modifying pathway enzymes, eliminating flux blockages due to byproduct synthesis, eliminating toxic byproducts, and optimizing via genome‐scale models. This review also provides insights on future directions for 3‐HP biosynthesis.

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“…Wang et al (2011) reported that the specific activity of glycerol dehydratase in K. pneumoniae at an aeration rate of 0.04 vvm was 59% higher than that in the absence of air supply. But, it has been reported that glycerol dehydratase can be rapidly inactivated by oxygen (Toraya 2000;Ruch and Lin 1975), and affected 3-HP production significantly (Xu et al 2009a;Huang et al 2013;Niu et al 2017). Additionally, coenzyme B12, the cofactor for DhaB, is not sufficiently synthesized under high aeration conditions in most of the natural 3-HPA producers such as K. pneumoniae.…”

Section: Effect Of Aeration On 3-hp Productionmentioning

confidence: 99%

“…Wild-type K. pneumoniae produces no or very little 3-HP, therefore, 3-HP production largely relies on the activity of ALDH, and insufficient supply of the cofactor NAD + also limits 3-HP production. Regeneration of NAD + is a critical factor for enhancing 3-HP production, and NAD + should be effectively regenerated for continuous 3-HP production (Niu et al 2017). Other advantages Fig.…”

Section: Introductionmentioning

confidence: 99%

Efficient 3-hydroxypropionic acid production from glycerol by metabolically engineered Klebsiella pneumoniae

Jiang

Huang

et al. 2018

Bioresour. Bioprocess.

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Background: 3-Hydroxypropionic acid (3-HP) is one of the top value-added chemicals that can be produced from renewable resources. Crude glycerol, a cheap feedstock, is the foremost by-product generated from biodiesel industries. In this study, Klebsiella pneumonia was engineered for efficient 3-HP production from glycerol.Results: Three metabolically engineered K. pneumoniae strains were constructed and the strain JJQ02 (ΔldhAΔdhaT) overexpressing aldehyde dehydrogenase from E. coli was the most suitable for 3-HP production. This strain produced 61.9 g/L of 3-HP (a yield of 0.58 mol/mol) in 38 h in a 5-L fermentor by control of the aeration rate. Deletion of ldhA and dhaT effectively eliminated lactate formation and reduced 1,3-propanediol production, but more 2,3-butanediol was accumulated due to redox balance. The fed-batch process was successfully scaled-up in a 300-L bioreactor where the 3-HP production level was 54.5 g/L (a yield of 0.43 mol/mol) in 51 h. Conclusions:Deficiency of both lactate dehydrogenase and 1,3-propanediol oxidoreductase as well as control of aeration significantly enhanced 3-HP production, and the successful scale-up of the fed-batch process in a 300-L bioreactor indicated a great potential to industrial production of 3-HP. which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Investigation of the key factors on 3-hydroxypropionic acid production with different recombinant strains

Cited by 6 publications

References 31 publications

Biological Production of 3-Hydroxypropionic Acid: An Update on the Current Status

Biological Production of 3-Hydroxypropionic Acid: An Update on the Current Status

Recent advances, challenges and metabolic engineering strategies in the biosynthesis of 3‐hydroxypropionic acid

Efficient 3-hydroxypropionic acid production from glycerol by metabolically engineered Klebsiella pneumoniae

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