To prepare antibacterial fabrics with simple approach, wood pulp/viscose fibers and amino-capped silver nanoparticles (Ag NPs) solution were utilized to form Ag Nps-coated wetlace nonwove fabric. Characterization of the Ag Nps and prepared wetlace nonwoven fabric was performed in virtue of TEM, UV-vis, XRD, ICP-AES, FESEM, EDS mapping and antibacterial test. FESEM and EDS characterizations demonstrated the hierarchical and uniform coating of high-density Ag NPs on wood pulp fibers, and antibacterial test indicated the excellent antibacterial activity of prepared wetlace nonwoven fabric.