Investigation of the mechanical effects of targeted drugs on cancerous cells based on atomic force microscopy

Zhu, Jiajing; Tian, Yanling; Wang, Zuobin; Wáng, Ying; Zhang, Wenxiao; Qu, Kaige; Weng, Zhankun; Liu, Xianping

doi:10.1039/d1ay00649e

Cited by 10 publications

(6 citation statements)

References 39 publications

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“…Indentation depth should not exceed 10% of the sample thickness. Arduino et al, 2022;Chen et al, 2021;Holuigue et al, 2022;Jamal et al, 2022;Liu et al, 2019;Tang et al, 2019 Parabolic Deptula et al, 2021;Han et al, 2022;Nardini et al, 2022;Nguyen & Liu, 2022;Offroy et al, 2020;Sun et al, 2021;Zhu et al, 2020;Zhu et al, 2021;Zhu et al, 2022;Zhu, Tian, et al, 2023 Pyramidal Any axisymmetric shape…”

Section: Model Indenter Shapementioning

confidence: 99%

Atomic force microscopy in disease‐related studies: Exploring tissue and cell mechanics

Liu,

Han,

Kong

et al. 2023

Microscopy Res & Technique

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Despite significant progress in human medicine, certain diseases remain challenging to promptly diagnose and treat. Hence, the imperative lies in the development of more exhaustive criteria and tools. Tissue and cellular mechanics exhibit distinctive traits in both normal and pathological states, suggesting that “force” represents a promising and distinctive target for disease diagnosis and treatment. Atomic force microscopy (AFM) holds great promise as a prospective clinical medical device due to its capability to concurrently assess surface morphology and mechanical characteristics of biological specimens within a physiological setting. This review presents a comprehensive examination of the operational principles of AFM and diverse mechanical models, focusing on its applications in investigating tissue and cellular mechanics associated with prevalent diseases. The findings from these studies lay a solid groundwork for potential clinical implementations of AFM.Research HighlightsBy examining the surface morphology and assessing tissue and cellular mechanics of biological specimens in a physiological setting, AFM shows promise as a clinical device to diagnose and treat challenging diseases.

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Section: Model Indenter Shapementioning

confidence: 99%

Atomic force microscopy in disease‐related studies: Exploring tissue and cell mechanics

Liu,

Han,

Kong

et al. 2023

Microscopy Res & Technique

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“…Furthermore, monitoring how mechanical and rheological properties evolve during, e.g. the action of anti-tumour drugs like the prostate [68], lung [46] or liver [118] cancers, makes it possible to use AFM to assess the effectiveness of the applied chemotherapy.…”

Section: Biomechanics Of Bladder Cancermentioning

confidence: 99%

Applicability of atomic force microscopy to determine cancer-related changes in cells

Lekka

2022

Phil. Trans. R. Soc. A.

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The determination of mechanical properties of living cells as an indicator of cancer progression has become possible with the development of local measurement techniques such as atomic force microscopy (AFM). Its most important advantage is a nanoscopic character, implying that very local alterations can be quantified. The results gathered from AFM measurements of various cancers show that, for most cancers, individual cells are characterized by the lower apparent Young's modulus, denoting higher cell deformability. The measured value depends on various factors, like the properties of substrates used for cell growth, force loading rate or indentation depth. Despite this, the results proved the AFM capability to recognize mechanically altered cells. This can significantly impact the development of methodological approaches toward the precise identification of pathological cells. This article is part of the theme issue ‘Nanocracks in nature and industry’.

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“…7,15,16 Data acquisition influences owing to the various experiment conditions, for instance, sample position (e.g. cell central body or periphery), 7 substrate stiffness, 17,18 loading force, 7,[19][20][21][22] loading rate, 7,19,16,21,22 loading time, 21,22 relaxation time, 23 biochemical and physical cues in surroundings 24 like temperature, 7 drugs 20,25,26 etc. Data analysis is partly influenced by the applied theoretical models.…”

Section: Introductionmentioning

confidence: 99%

“…15 Weber et al determined the mechanical response of endothelial cells by employing loading rates of 0.5−20 μm/s and loading force of 100−10,000 pN with a sharp, four-sided pyramidal indenter and indicated that the increase of stiffness with higher loading rates and higher loading force. 19 Martens et al investigated the effects of the blebbistatin and Y-27632 drugs on fibroblast cells for 30-60 min using AFM and concluded that the blebbistatin changed the morphological and mechanical properties whereas Y-27532 did not present any visible effects. 20 Mathur et al identified that the elastic properties of three various cell types vary significantly (E endothelial cells < E cardiac muscle cells < E skeletal muscle cells ) and the relationship between the elastic response to the probe geometry had also been explored.…”

Section: Introductionmentioning

confidence: 99%

The effects of measurement parameters on the cancerous cell nucleus characterisation by atomic force microscopy in vitro

Zhu

Tian

Yan

et al. 2022

Journal of Microscopy

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Cancer is now responsible for the major leading cause of death worldwide. It is noteworthy that lung cancer has been recognised as the highest incidence (11.6%) and mortality (18.4%) for combined sexes among a variety of cancer diseases. Therefore, it is of great value to investigate the mechanical properties of lung cancerous cells for early diagnosis. This paper focus on the influence of measurement parameters on the measured central Young's moduli of single live A549 cell in vitro based on the force spectroscopy mode of atomic force microscopy (AFM). The effects of the measurement parameters on the measured central Young's moduli were analysed by fitting the force–depth curves utilising the Sneddon model. The results revealed that the Young's moduli of A549 cells increased with the larger indentation force, higher indentation speed, less retraction time, deeper Z length and lower purity percentage of serum. The Young's moduli of cells increased first and then decreased with the increasing dwell time. Hence, this research may have potential significance to provide reference for the standardised detection of a single cancerous cell in vitro using AFM methodologies.

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Investigation of the mechanical effects of targeted drugs on cancerous cells based on atomic force microscopy

Abstract: Cancer is currently drawing more and more attention worldwide as the leading factor of death worldwide. However, few research directed towards the mechanical properties of cancer cells treated by targeted...

Cited by 10 publications

References 39 publications

Atomic force microscopy in disease‐related studies: Exploring tissue and cell mechanics

Atomic force microscopy in disease‐related studies: Exploring tissue and cell mechanics

Applicability of atomic force microscopy to determine cancer-related changes in cells

The effects of measurement parameters on the cancerous cell nucleus characterisation by atomic force microscopy in vitro

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