Investigation of the mechanism of accelerated biodegradation ofParacoccus-KDSPL-02

Abstract

Paracoccus-KDSPL-02 can accelerate to degrade penicillin G under light remain poorly understood, largely due to the lack of high-throughput genome engineering tools. Firstly, this study sequenced the genome of Paracoccus-KDSPL-02 and mined the genes that might be involved, and in order to understand in detail whether the expression of the mined genes changed during light. Further, for genes with altering transcriptional levels under light, this study obtained PROKKA_01468 which a photoreceptor protein in Paracoccus-KDSPL-02. In the end, for validating the function of PROKKA_01468, this study knocked down the sequence of the PROKKA_01468 by applying gene editing system, and the knockdown strain showed significant change in the rate of degradation of phenylacetic acid, which is the intermediate product of penicillin G degradation, by light compared with darkness, so that the PROKKA_01468 is the most effective photoreceptor protein in Paracoccus-KDSPL-02