Investigation of the Oxidative Stress Response of a Green Synthesis Nanoparticle (RP-Ag/ACNPs) in Zebrafish

Köktürk, Mine; Yıldırım, Serkan; Nas, Mehmet Salih; Özhan, Günes; Atamanalp, Muhammed; Bolat, İsmail; Çalımlı, Mehmet Harbi; Alak, Gonca

doi:10.1007/s12011-021-02855-3

Cited by 16 publications

(6 citation statements)

References 58 publications

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“…TEM images of silver nanoparticles have shown that the morphology of silver nanoparticles was predominantly quasi-spherical with a mean diameter of 12.3 nm and a standard deviation 2.56 nm. These results are similar to those obtained by other authors using the same methodology 51 – 53 . This is due to the bioreduction from various compounds in the medium, as commonly occurs in green synthesis.…”

Section: Discussionsupporting

confidence: 92%

Biogeneration of silver nanoparticles from Cuphea procumbens for biomedical and environmental applications

González-Pedroza

Benítez

Navarro-Marchal

et al. 2023

Sci Rep

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Nanotechnology is one of the most important and relevant disciplines today due to the specific electrical, optical, magnetic, chemical, mechanical and biomedical properties of nanoparticles. In the present study we demonstrate the efficacy of Cuphea procumbens to biogenerate silver nanoparticles (AgNPs) with antibacterial and antitumor activity. These nanoparticles were synthesized using the aqueous extract of C. procumbens as reducing agent and silver nitrate as oxidizing agent. The Transmission Electron Microscopy demonstrated that the biogenic AgNPs were predominantly quasi-spherical with an average particle size of 23.45 nm. The surface plasmonic resonance was analyzed by ultraviolet visible spectroscopy (UV–Vis) observing a maximum absorption band at 441 nm and Infrared Spectroscopy (FT IR) was used in order to structurally identify the functional groups of some compounds involved in the formation of nanoparticles. The AgNPs demonstrated to have antibacterial activity against the pathogenic bacteria Escherichia coli and Staphylococcus aureus, identifying the maximum zone of inhibition at the concentration of 0.225 and 0.158 µg/mL respectively. Moreover, compared to the extract, AgNPs exhibited better antitumor activity and higher therapeutic index (TI) against several tumor cell lines such as human breast carcinoma MCF-7 (IC50 of 2.56 µg/mL, TI of 27.65 µg/mL), MDA-MB-468 (IC50 of 2.25 µg/mL, TI of 31.53 µg/mL), human colon carcinoma HCT-116 (IC50 of 1.38 µg/mL, TI of 51.07 µg/mL) and melanoma A-375 (IC50 of 6.51 µg/mL, TI of 10.89 µg/mL). This fact is of great since it will reduce the side effects derived from the treatment. In addition, AgNPs revealed to have a photocatalytic activity of the dyes congo red (10–3 M) in 5 min and malachite green (10–3 M) in 7 min. Additionally, the degradation percentages were obtained, which were 86.61% for congo red and 82.11% for malachite green. Overall, our results demonstrated for the first time that C. procumbens biogenerated nanoparticles are excellent candidates for several biomedical and environmental applications.

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Section: Discussionsupporting

confidence: 92%

Biogeneration of silver nanoparticles from Cuphea procumbens for biomedical and environmental applications

González-Pedroza

Benítez

Navarro-Marchal

et al. 2023

Sci Rep

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“…Or it can be explained by the decreased egg hatching rate, with the used metals (Pd-Cu) limiting the physical movement of the embryos and interfering with the activities of the hatching enzymes necessary for the embryo to digest the chorion. [9,[43][44][45][46][47] The previous studies with different metallic NPs had also stated that the hatchability of D. rerio embryos is negatively affected (nano-palladium, [27] Ag NPs, [48] gold and silver NPs, [49] RP-Ag/ACNPs, [47] gold F I G U R E 6 Microscopic images of the abnormalities observed in zebrafish embryos/larvae at 96 hpf. Black star: yolk sac edema, red arrow: lordosis/kyphosis, red star: pericardial edema, blue star: tail malformation, yellow star: microphthalmia scale bar: 0.5 mm (30X) F I G U R E 7 Morphological deformations in zebrafish embryos/ larvae exposed to different concentrations of PdCu@f-MWCNT nanoparticles (NPs).…”

Section: Discussionmentioning

confidence: 98%

“…Or it can be explained by the decreased egg hatching rate, with the used metals (Pd‐Cu) limiting the physical movement of the embryos and interfering with the activities of the hatching enzymes necessary for the embryo to digest the chorion. [ 9,43–47 ] The previous studies with different metallic NPs had also stated that the hatchability of D. rerio embryos is negatively affected (nano‐palladium, [ 27 ] Ag NPs, [ 48 ] gold and silver NPs, [ 49 ] RP‐Ag/ACNPs, [ 47 ] gold NPs, RP‐Pd/AC NPs [ 9 ] ). As expected, a very small particle size will facilitate the passage of the particle through the cell membrane.…”

Section: Discussionmentioning

confidence: 99%

“…While low doses of some of them exhibit positive effects, the studies had exhibited that exposure to metal NPs at high levels causes toxic harm to the organism. [43][44][45][46][47] It was determined that the survival rate of embryos exposed to PdCu@f-MWCNT NPs decreased with increasing dose. This may have resulted from the interaction of Pd and Cu metals with hatching enzymes to digest the chorion.…”

Section: Discussionmentioning

confidence: 99%

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May PdCu@f‐MWCNT NPs be an ecotoxicologic risk?

Alak

Yeltekin

Köktürk

et al. 2023

Applied Organom Chemis

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The mechanisms of various metal nanoparticles (NPs) are still a matter of debate in aquatic toxicology. In order to close this gap, the number of studies on this subject is increasing. The aims of this study are to explore the interactions of PdCu@f-MWCNT NPs with zebrafish and get to know if it has an ecotoxicological risk. In this study, we synthesized, characterized PdCu@f-MWCNT NPs, and investigated its ecotoxicological effects. The chemical and morphological structures of PdCu@f-MWCNT nanomaterials were elucidated with advanced analysis techniques such as scanning electron microscope (SEM), X-ray spectrophotometer (EDX), transmission electron microscope (TEM), and X-ray diffraction (XRD) analyses. The ecotoxicological risks were investigated by conducting biochemical analyses and malformation defects in zebrafish. The results were obtained using multiple marker applications including oxidative stress parameters, proinflammatory cytokine expressions, DNA damage, and apoptosis markers. The obtained findings showed embryonic development, survival rate, and incidence of malformation increasing in parallel with the dose increase in zebrafish exposed to PdCu@f-MWCNT NPs at 10-1000 μg/L levels. Despite the observed decreases in antioxidant enzyme activities, GSH level, AChE activity, and NRF-2 level, inductions in malondialdehyde (MDA) level, IL-6, TNF-α, NF-kB, DNA damage, and apoptosis levels were observed with increasing dose. Considering these findings, it has been determined that there are toxic effects in zebrafish at all levels above the concentration that we examine as a safe dose (10 μg/L). The effects of all studied concentrations of this NP above the safe dosage, especially on oxidative stress, malformation, and immune system, were found to be quite significant. The more widespread use of nanotechnology in the future will mean more contact of aquatic organisms with NPs. As a result, it is clearly seen that these organisms, which form the important link of the food chain, and the aquatic environment are living at risk conditions.

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“…The intelligent selection of biomes/biome-extracted precursors for nanosystem fabrication can be used to control the surface functionalities, optimizing band structures and band gaps, morphological and topological features, ambient and hostile environment stabilities, biocompatibility, toxicity and bioaccumulation for targeted solutions. In recent years, various targeted green nanosystems have been engineered for combatting NDDs, including implantable and diagnostic devices like catheters, biosensors and stunts, drug delivery like nanoliposomes with potential to be functionalized with antibodies and crossing BBB, developing immunity and nanoscaffolds for neurogeneration, controlling and curing primary and secondary symptoms, biocompatibility in surgical prostheses, and targeted therapeutics and imaging eliminating systemic toxicity (Mizrahi et al, 2014;Santhoshkumar et al, 2014;Charbgoo et al, 2017;Maddinedi et al, 2017;Suganthy et al, 2018;Kotcherlakota et al, 2019;Zhang et al, 2020;Ahlawat and Narayan, 2021;Jan et al, 2021;Pathania et al, 2022bPathania et al, , 2022aCui et al, 2022;Jasim et al, 2022;Kokturk et al, 2022;Monika et al, 2022). Moreover, their indirect utilization for strengthening present NDD monitoring strategies, such as enhancing the monitoring performance of microelectrodes utilized in electrophysiology, improving lasermediated vascular anastomoses, and enhancing optical interfaces for neural imaging, is combatting the bottlenecks of controlled monitoring of neuronal ensembles.…”

Section: Emergence Of Green Nanosystems For Efficient Ndd Management:...mentioning

confidence: 99%

Prospects of green nanotechnology for efficient management of neurodegenerative diseases

Chaudhary

2022

Front. Nanotechnol.

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Current theranostics for neurodegenerative diseases (NDD) management are majorly symptomatic due to a lack of identification of early-stage biomarkers and the inefficiency of drugs to penetrate through the blood-brain barrier. Recently, the Neuro-nanotechnology interface has emerged as a potential strategy for diagnosis, monitoring, and treatment of NDDs owing to smaller particle size, high specific surface area, tunable physicochemical attributes and rich surface functionalities. However, toxicity and biocompatibility are two significant challenges restricting their commercial prospect in NDD management. On the contrary, green nanosystems fabricated using plant extracts, microorganisms, biome-based precursors, repurposed-byproducts, exosomes, and protein-based bio-nanomaterials are economical, eco-friendly, biocompatible and renewable due to the abundance of biodiversity. This prospect explores the novel and cutting-edge interface of green nanosystems and NDDs for developing diagnostic and implantable devices, targeted drug delivery strategies, surgical prostheses, therapeutics, treatment, nanoscaffolds for neurogeneration, and immunity development. Besides, it discusses the challenges, alternate solutions and advanced prospects of green nanosystems with the integration of modern-age technologies for the development of sustainable green Neuro-nanotechnology for efficient management of NDDs.

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Investigation of the Oxidative Stress Response of a Green Synthesis Nanoparticle (RP-Ag/ACNPs) in Zebrafish

Cited by 16 publications

References 58 publications

Biogeneration of silver nanoparticles from Cuphea procumbens for biomedical and environmental applications

Biogeneration of silver nanoparticles from Cuphea procumbens for biomedical and environmental applications

May PdCu@f‐MWCNT NPs be an ecotoxicologic risk?

Prospects of green nanotechnology for efficient management of neurodegenerative diseases

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