Investigation of the Pathogenic Mechanisms in Regional Enteritis With in vitro Lymphocyte Cultures

Parent, Kevin; Barrett, Judith; Wilson, I. Dodd

doi:10.1016/s0016-5085(19)33492-4

Cited by 51 publications

(14 citation statements)

References 29 publications

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“…The present finding of impaired DNCB responses, for example, agrees closely with the earlier data of Jones et al (1969). Similarly, impaired lymphocyte response to phytohaemagglutinin has been reported in several studies of CD (Walker and Greaves, 1969;Brown et al, 1970;McHattie et al, 1971;Parent et al, 1971;Asquith et al, 1973), as have decreased levels of circulating T-lymphocytes (Strickland et al, 1974) and impaired cutaneous responsiveness to a broad spectrum of antigens (Meuwissen et al, 1975). These previous studies, like ours, have demonstrated no relationship between impaired immunity and the duration, extent, or severity of the bowel disease.…”

Section: Discussionsupporting

confidence: 87%

“…Some possible technical reasons for apparent lack of impaired PHA-responsiveness in other studies (Hinz et al, 1967;Stefani and Fink, 1967;Parent et al, 1971;Aas et al, 1972;Asquith et al, 1973;Bolton et al, 1974) have been discussed by us previously (Sachar et al, 1973;Sachar and Taub, 1975). Other claims of normal cellular immunity in ulcerative colitis have been based upon results of conventional skin testing with mumps, coccidioidin, and trichophyton (Binder et al, 1966) as well as with tuberculin (Bolton et al, 1974).…”

Section: Discussionmentioning

confidence: 75%

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Anergy to dinitrochlorobenzene and depression of T-lymphocytes in Crohn's disease and ulcerative colitis.

Meyers¹,

Sacher²,

Taub³

et al. 1976

Gut

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SUMMARY Skin reactivity to dinitrochlorobenzene (DNCB) and levels of circulating T-lymphocytes were measured in 15 patients with ulcerative colitis, 15 patients with Crohn's disease, and 12 normal control subjects. Diminished reactivity to DNCB was demonstrated in 87 % of patients with Crohn's disease (p < 0 001) and in 53 % with ulcerative colitis (p < 0 02), as compared with only 8-5 % of controls; anergy was more frequent in Crohn's disease than in ulcerative colitis (p < 0 05

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Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 75%

Anergy to dinitrochlorobenzene and depression of T-lymphocytes in Crohn's disease and ulcerative colitis.

Meyers¹,

Sacher²,

Taub³

et al. 1976

Gut

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“…In general, studies in ulcerative colitis patients have not revealed a depression of immune competence (Hinz, Perlmann, and Hammarstrom, 1967;Parent, Barrett, and Wilson, 1971). In contrast, patients with Crohn's disease have shown a high incidence of negative Mantoux responses (Blackburn, Hadfield, and Hunt, ,1939;Phear, 1958;Williams, 1965), as well as diminished cutaneous delayed hypersensitivity reactions to dinitrochlorobenzene (Jones, Housley, Ashurst, and Hawkins, 1969).…”

mentioning

confidence: 99%

The immune competence of patients with inflammatory bowel disease

Bolton¹,

James²,

Newcombe³

et al. 1974

Gut

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“…Using different methods of lymphocyte separation a number of workers have shown normal PHA-induced lymphoblastic transformation in patients with active ulcerative colitis. (Hinz, Perlmann, and Hammarstrom, 1937;Parent, Barrett, and Wilson, 1971;Aas, Huizenga, Newcomer, and Shorter, 1972).…”

Section: Discussionmentioning

confidence: 99%

“…In other studies antigenic stimulants have included extracts of foetal colon, diseased large bowel, normal colon, germ-free rat faeces, and strains of E. coli. While one group of investigators (Stefani and Fink, 1967) have reported a decreased response to various antigens, others have shown normal responses in patients with active ulcerative colitis when compared with normal subjects (Hinz et al, 1967;Parent et al, 1971). In our study, using viable autologous rectal epithelial cells as a stimulant, a significantly greater response was found in acute severe ulcerative colitis when compared with findings in normal subjects (fig 3).…”

Section: Discussionmentioning

confidence: 99%

Lymphoblastic response to autologous colon epithelial cells in ulcerative colitis in vitro

Hunt¹,

Trotter²

1973

Gut

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SUMMARY The blastic transformation in vitro of peripheral blood lymphocytes was measured by the 72-hour uptake of tritiated thymidine (3H-6-thymidine) in 23 patients with mucosal ulcerative colitis, three patients with acute Crohn's colitis with rectal involvement, and seven normal subjects. The 23 patients with ulcerative colitis were subdivided into three groups, graded according to severity into seven with acute, severe, nine with active, chronic, and seven with quiescent disease.In the control cultures of lymphocytes without any added potential stimulant the uptake of 3H-6-thymidine in the clinical subgroup of seven patients with acute, severe ulcerative colitis was significantly greater than in seven normal subjects (P <001). This contrasted with a reduced uptake of 3H-6-thymidine by lymphocytes from seven patients with acute severe colitis when compared with seven normal subjects after stimulation with phytohaemagglutinin-P (PHA-P) (P < 0.01).In further duplicate cultures of lymphocytes specifically stimulated by an equal number of viable autologous rectal epithelial cells, the uptake of 3H-6-thymidine was significantly greater in seven patients with acute severe colitis when compared with seven normal subjects (P < 0.01). The results in three patients with acute Crohn's colitis with rectal involvement showed no such evidence of lymphocyte sensitivity to autologous rectal epithelial cells and their uptake of 3H-6-thymidine lay within the normal range.Evidence that the degree of lymphoblastic transformation was related to the clinical severity of ulcerative colitis was provided by the results obtained in the unstimulated and epithelial cell stimulant cultures. The uptake of 3H-6-thymidine was directly related to the clinical severity of ulcerative colitis in the three subgroups studied.In addition, four of the seven patients with acute severe colitis were studied later in clinical remission. They were then found to have a significantly reduced uptake of 3H-6-thymidine in response to autologous rectal epithelial cells (P < 0.01).In the normal cellular immune response blastic transformation of thymically directed lymphocytes is an important component of the process of recognition of certain antigens by the host. This transformation leads to the production of a group of effector cells which mediate a specific response against that antigen (Roitt, Greaves, Torrigiani, Brostoff, and Playfair, 1969). Lymphocytes from patients with ulcerative colitis have been found to damage autologous colon epithelial cells in culture

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Investigation of the Pathogenic Mechanisms in Regional Enteritis With in vitro Lymphocyte Cultures

Cited by 51 publications

References 29 publications

Anergy to dinitrochlorobenzene and depression of T-lymphocytes in Crohn's disease and ulcerative colitis.

Anergy to dinitrochlorobenzene and depression of T-lymphocytes in Crohn's disease and ulcerative colitis.

The immune competence of patients with inflammatory bowel disease

Lymphoblastic response to autologous colon epithelial cells in ulcerative colitis in vitro

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