Investigation of the Presence of Encephalitozoon intestinalis and Enterocytozoon bieneusi in Bone Marrow Transplant Patients by IFA-MAbs Method

Çetinkaya, Ülfet; Hamamcı, Berna; Kaynar, Leylagül; Kuk, Salih; Şahi̇n, İzzet; Yazar, Süleyman

doi:10.5578/mb.9809

Cited by 10 publications

(2 citation statements)

References 6 publications

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“…It was thought that the prevalence difference may be due to the diagnostic approaches since Real Time PCR is more sensitive than fluorescence microscopy. A lower prevalence value of 4% for E. bieneusi in bone marrow transplant patients was also reported using a commercial immunofluorescence antibody test [ 13 ]. In another study conducted in Türkiye, the prevalence of E. bieneusi was reported as 9,1% in patients with diarrhea using multiplex nested PCR method [ 15 ].…”

Section: Discussionmentioning

confidence: 92%

“…Although, D and Type IV, ERUSS1, and BEB6 as well as new genotypes were identified in different animal species in studies conducted in Türkiye [10][11][12], there are several studies that investigated the prevalence of E. bieneusi in humans living in Türkiye but these studies do not provide any information about the genotype profile of E. bieneusi [13][14][15]. Moreover, a study reported that prevalence of microsporidia (including Encephalitozoon hellem/intestinalis, Encephalitozoon cuniculi, Encephalitozoon sp., E. bieneusi) in colon cancer patients was 41,3% whereas the prevalence in healthy controls was %0 [16].…”

Section: Introductionmentioning

confidence: 99%

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Molecular prevalence and genotypes of Enterocytozoon bieneusi in cancer patients under chemotherapy in Aegean region of Türkiye

Aksoy Gökmen,

Öncü Öner,

Erkunt Alak

et al. 2024

BMC Microbiol

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Background Enterocytozoon bieneusi is the most common species found in humans. Although E. bieneusi has been investigated in humans, genotype profile of E. bieneusi is not known in Türkiye. Methods In this study, we screened E. bieneusi in patients (n = 94) with different types of malignant solid tumors by Real Time PCR and then sequenced E. bieneusi positive samples. All cancer patients were undergoing chemotherapy and had diarrhea. Moreover, as control groups, we also screened E. bieneusi in patients with diarrhea (n = 50) and without diarrhea (n = 50). Results Among all patients analyzed, 33 (17%) were found to be E. bieneusi-positive. As the patients were categorized, the molecular prevalence of E. bieneusi increased to 25.5% among cancer patients with diarrhea. However, the molecular prevalence of E. bieneusi was found to be lower in patients with presenting only diarrhea (8%) and patients without diarrhea (10%). The high molecular prevalence value detected among cancer patients with diarrhea was also statistically significant compared to other patient groups (P = 0.00112 and P = 0.0269). Among the 33 Real Time PCR positive samples, 10 of them were amplified by nested PCR and among these 10 samples, 6 of them were successfully genotyped. The phylogenetic tree showed the presence of D and Type IV which were also identified in stray cats living in İzmir in our previous study. Conclusions High molecular prevalence value indicates the importance of screening stool samples of cancer patients with diarrhea for E. bieneusi and genotyping results indicate that D and Type IV are circulating between humans and cats.

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Section: Discussionmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Molecular prevalence and genotypes of Enterocytozoon bieneusi in cancer patients under chemotherapy in Aegean region of Türkiye

Aksoy Gökmen,

Öncü Öner,

Erkunt Alak

et al. 2024

BMC Microbiol

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Evaluation of Immunoflourescence antibody assay for the detection of Enterocytozoon bieneusi and Encephalitozoon intestinalis

et al. 2016

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Detection of microsporidia at the species level is important for therapeutic purpose. The available techniques, modified trichrome (MT) staining cannot differentiate between species, while polymerase chain reaction (PCR) requires a reference laboratory and skilled technical staff. Immunoflourescence antibody (IFA) assay is another technique, which can differentiate among commonest species of microsporidia. However, there are very limited studies on its efficacy worldwide. Therefore, we aimed to evaluate IFA assay for the detection of microsporidia and differentiation among commonest species, Enterocytozoon bieneusi (E. bieneusi) and Encephalitozoon intestinalis infecting immunocompromised patients. Stool samples from 200 immunocompromised patients (19 with microsporidia and 181 without microsporidia using MT staining) were tested for species identification by PCR-RFLP and IFA assay. Sensitivity, specificity, diagnostic accuracy, and positive and negative predictive values were calculated as per standard formulae. Kappa statistics was used to assess the agreement between three tests. Of 200 immunocompromised patients, 21 and 20 patients had microsporidia using PCR and IFA assay, respectively. IFA assay and PCR identified E. bieneusi in all patients infected with microsporidia. Considering MT stain as gold standard, sensitivity and specificity of IFA assay was 100 and 99.4 %, respectively. Upon considering PCR as gold standard, sensitivity and specificity of IFA assay was 95.2 and 100 %, respectively. Diagnostic accuracy of IFA assay was 99.5 % along with its high test agreement with MT staining and PCR (K = 0.915, p = 0.049; K = 0.973, p = 0.027). IFA assay is highly sensitive and specific technique for detecting and identifying species of microsporidia among immunocompromised patients. E. bieneusi was the commonest species identified.

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Encephalitozoon intestinalis Infection Impacts the Expression of Apoptosis-Related Genes in U937 Macrophage Cells

et al. 2020

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Investigation of the Presence of Encephalitozoon intestinalis and Enterocytozoon bieneusi in Bone Marrow Transplant Patients by IFA-MAbs Method

Cited by 10 publications

References 6 publications

Molecular prevalence and genotypes of Enterocytozoon bieneusi in cancer patients under chemotherapy in Aegean region of Türkiye

Molecular prevalence and genotypes of Enterocytozoon bieneusi in cancer patients under chemotherapy in Aegean region of Türkiye

Evaluation of Immunoflourescence antibody assay for the detection of Enterocytozoon bieneusi and Encephalitozoon intestinalis

Encephalitozoon intestinalis Infection Impacts the Expression of Apoptosis-Related Genes in U937 Macrophage Cells

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