Investigation of the probiotic and metabolic potential of Fructobacillus tropaeoli and Apilactobacillus kunkeei from apiaries

Şimşek, Duygu; Kiymaci, Merve Eylul; Tok, Kenan Can; Gümüştaş, Mehmet; Altanlar, Nurten

doi:10.1007/s00203-022-03000-x

Cited by 10 publications

(6 citation statements)

References 42 publications

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“…To evaluate the resistance of the strain to pepsin, PBS buffer was adjusted to pH 3.0 and 2.0, and 3 mg/mL of pepsin was added. PBS buffer was inoculated with 1% of the active culture (adjusted to 0.5 McFarland) and incubated at 37°C for 3 h (Simsek et al., 2022 ). CFU and strain survival rate were calculated at the beginning, and at the end of 1 and 3 h of incubation.…”

Section: Methodsmentioning

confidence: 99%

“…To determine the pancreatin resistance of the strain, 1 mg/mL pancreatin was added to PBS buffer with a pH of 8.0. PBS buffer was inoculated with 1% of the active culture (adjusted to 0.5 McFarland) and incubated at 37°C for 4 h (Simsek et al., 2022 ). CFU at baseline and at 4 h and the survival rate of the strain were determined.…”

Section: Methodsmentioning

confidence: 99%

“…McFarland) and incubated at 37°C for 3 h (Simsek et al, 2022). CFU and strain survival rate were calculated at the beginning, and at the end of 1 and 3 h of incubation.…”

Section: Pepsin Resistancementioning

confidence: 99%

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Evaluating Enterococcus faecium9 N‐2 as a probiotic candidate from traditional village white cheese

Dikbaş,

Orman,

Alım

et al. 2023

Food Science & Nutrition

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In this study, various functional and probiotic attributes of the Enterococcus faecium 9 N‐2 strain isolated from village‐style white cheese were characterized, while also assessing its safety. To achieve this, we conducted an in vitro analysis of several key probiotic properties exhibited by the 9 N‐2 strain. Notably, this strain demonstrated robust resilience to low pH, high bile salt concentrations, lysozyme, pepsin, pancreatin, and phenol. Furthermore, this strain displayed exceptional auto‐aggregation capabilities and moderate co‐aggregation tendencies when interacting with Escherichia coli. The cell‐free supernatant derived from strain 9 N‐2 exhibited significant antimicrobial activity against the tested pathogens. The strain exhibited resistance to gentamicin, meropenem, and bacitracin, while remaining susceptible to vancomycin and various other antibiotics. Furthermore, it was found that E. faecium 9 N‐2 possessed the capacity to produce the phytase enzyme. When all the results of this study are evaluated, it is thought that 9 N‐2 strain has superior probiotic properties, and therefore it can be used as probiotic in food, medicine, and animal feed in the future. In addition, further in vivo tests should be performed to fully understand its effects and mechanisms of action and to confirm its safety and probiotic effects. Further research and clinical trials are also needed to identify new strains with potential probiotic properties.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Evaluating Enterococcus faecium9 N‐2 as a probiotic candidate from traditional village white cheese

Dikbaş,

Orman,

Alım

et al. 2023

Food Science & Nutrition

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“…In particular, several studies have highlighted the benefits of honeybee-associated LAB [ 36 , 37 , 38 , 39 , 40 ]. While most of these studies have demonstrated the effectiveness of probiotics in protecting hosts [ 41 , 42 ], their ability to safeguard the skin or inhibit melanin synthesis remains unexplored. This study explored the antioxidative and antimelanogenic properties of Lactobacillus kunkeei culture product on skin cells, shedding light on the skin protection potential of honeybee-associated LAB.…”

Section: Introductionmentioning

confidence: 99%

Antioxidant and Antimelanogenic Activities of Lactobacillus kunkeei NCHBL-003 Isolated from Honeybees

Lee,

Choi,

Kang

et al. 2024

Microorganisms

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Excessive reactive oxygen species production can detrimentally impact skin cell physiology, resulting in cell growth arrest, melanogenesis, and aging. Recent clinical studies have found that lactic acid bacteria have a special effect directly or indirectly on skin organs, but the exact mechanism has not been elucidated. In this study, we investigated the mechanisms underlying the antioxidant protective effect and the inhibitory effect on melanin synthesis of Lactobacillus kunkeei culture supernatant (CSK), isolated from Apis mellifera Linnaeus (the Western honeybee). CSK exhibited notable efficacy in promoting cell migration and wound healing under oxidative stress, surpassing the performance of other strains. CSK pretreatment significantly upregulated the expression of Nrf2/HO-1 (nuclear factor erythroid 2-related factor 2/heme oxygenase-1), a key player in cellular defenses against oxidative stress, relative to the control H2O2-treated cells. The DCF-DA (dichloro-dihydro-fluorescein diacetate) assay results confirmed that CSK’s ability to enhance Nrf2 and HO-1 expression aligns with its robust ability to remove H2O2-induced reactive oxygen species. Furthermore, CSK upregulated MAPK (mitogen-activated protein kinase) phosphorylation, an upstream signal for HO-1 expression, and MAPK inhibitors compromised the wound-healing effect of CSK. Additionally, CSK exhibited inhibitory effects on melanin synthesis, downregulating melanogenesis-related genes in B16F10 cells. Thus, the present study demonstrated that CSK exhibited antioxidant effects by activating the Nrf2/HO-1 pathway through MAPK phosphorylation, thereby restoring cell migration and demonstrating inhibitory effects on melanin production. These findings emphasize the antioxidant and antimelanogenic potential of CSK, suggesting its potential use as a therapeutic agent, promoting wound healing, and as an active ingredient in skin-lightening cosmetics.

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“…Apilactobacillus, which were formerly classified as Lactobacillus, have recently been reclassified to underline specific adaptation to bees. Indeed, Apilactobacillus kunkeei (basonym Lactobacillus kunkeei) is an important component of the gut microbiota of honeybees and is considered as probiotic for these insects due to the bacteriocin-like substances produced in the honeybee gut, among other properties (Simsek et al, 2022). Enterococcus hirae, on the other hand, is a LAB species isolated from dairy products, and has been reported to exhibit probiotic potential (Melo et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Preliminary study of the effects of Apilactobacillus kunkeei EIR/BG-1 and Enterococcus hirae EIR/CM-2 supplementation on in vitro rumen fermentation and microbial population

Demirtas,

Sevin,

Musa

et al. 2023

J. Anim. Feed Sci.

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Investigation of the probiotic and metabolic potential of Fructobacillus tropaeoli and Apilactobacillus kunkeei from apiaries

Cited by 10 publications

References 42 publications

Evaluating Enterococcus faecium9 N‐2 as a probiotic candidate from traditional village white cheese

Evaluating Enterococcus faecium9 N‐2 as a probiotic candidate from traditional village white cheese

Antioxidant and Antimelanogenic Activities of Lactobacillus kunkeei NCHBL-003 Isolated from Honeybees

Preliminary study of the effects of <i>Apilactobacillus kunkeei</i> EIR/BG-1 and <i>Enterococcus hirae</i> EIR/CM-2 supplementation on<i> in vitro</i> rumen fermentation and microbial population

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