Investigation of the Relationship between Breast Cancers ‎and HPVs

et al. 2021

Virol J

Background The role of viruses as a cause of breast cancer (BC) has been significantly investigated in recent years. Human papillomavirus (HPV) has been detected in invasive breast carcinomas, while most studies have only focused on the detection of viral DNA, we aimed to examine the prevalence and genotypes of HPV among Iranian BC patients. We also examined the presence of herpes simplex-1 (HSV-1), herpes simplex-2 (HSV-2), varicella zoster virus (VZV), and cytomegalovirus (CMV) in these samples. Methods We collected and analyzed 70 Formalin-Fixed Paraffin-Embedded (FFPE) blocks including 59 BC samples, and 11 benign breast lesions as control from Iranian patients using nested PCR. Real-time PCR utilized as a confirming test to nested PCR findings. Genotyping of HPV positive samples was performed, the samples were also subjected to a multiplex PCR to detect HSV-1, HSV-2, VZV, and CMV in BC. Results Papillomavirus DNA was present in 7 of 59 BC samples (11.8%); while none was detected in control samples. The most prevalent type was HPV18, followed by HPV 6. All HPV positive patients had high tumor grades (II/ III) with a histologic diagnosis of ductal carcinoma. The patient age range was 33 to 73 years with a median of 51 years. Most of HPV positive patients had low levels of education. HPV16 was not detected. Also, 5 of 59 BC specimens (8.47%), were positive for HSV-1. But none of the samples were positive for HSV-2, VZV, and CMV. Conclusions Our results suggest a carcinogenesis role for High-risk HPV (HPV18) in breast tumors. Our findings of HSV-1 and low-risk HPV (HPV6) in BCs may propose a cancer-causing role for them. Further large-scale studies are warranted to assess the significance of our findings.

Section: Introductionmentioning

confidence: 99%

Detection of human papillomavirus genotypes, herpes simplex, varicella zoster and cytomegalovirus in breast cancer patients

et al. 2021

Virol J

“…For the rst time in 1992 Di Lonardo A and colleagues suggested that HPV could play a crucial role in the genesis of breast carcinoma by detecting HPV16 DNA sequence in 29.4% of breast and lymph node samples (28). Following the rst publication, several more studies have documented the presence of HPV in malignant breast tumors (29)(30)(31). On the contrary negative relationship between HPV and breast cancer has been declared by few studies (32)(33)(34).…”

Section: Introductionmentioning

confidence: 99%

Detection of Human Papillomavirus Genotypes in Breast Cancer Patients

et al. 2020

Preprint

Background: The role of viruses as a cause of breast cancer (BC) have been significantly investigated in recent years. Human papillomavirus (HPV) has been detected in invasive breast carcinomas, while most studies have only focused on the detection of viral DNA, we aimed to determine genotypes of the culprit HPV's. Methods: We collected and analyzed 70 Formalin Fixed Paraffin Embedded (FFPE) blocks including 59 BC samples, and 11 benign breast lesions as control from Iranian patients using nested PCR. Real-time PCR utilized as a confirming test to nested PCR findings. Genotyping of HPV positive samples was performed, the samples were also subjected to a multiplex PCR to detect herpes simplex-1 (HSV-1), herpes simplex-2 (HSV-2), varicella zoster virus (VZV) and cytomegalovirus (CMV) in BC.Results: Papillomavirus DNA was present in 7 of 59 BC samples (11.8%); while none was detected in control samples. The most prevalent type was HPV18, followed by HPV 6. No HPV16 genotype was detected .All HPV positive patients had high tumor grades (II/ III) with histologic diagnosis of ductal carcinoma. The patient age range was 33 to 73 years with median of 51 years. Most of HPV positive patients had low levels of education. Also 5 of 59 BC specimens (8.47%), were positive for HSV-1.Conclusions: Our results suggest a carcinogenesis role for High risk HPVs in breast tumors. Our findings of HPV6 and HSV1 genotypes in BC samples, propose a cancer causing role for low risk HPVs and herpes viruses. Further large scale studies are warranted to assess the significance of our findings.

“…For the rst time in 1992 Di Lonardo A and colleagues suggested that HPV could play a crucial role in the genesis of breast carcinoma by detecting HPV16 DNA sequence in 29.4% of breast and lymph node samples (28). Following the rst study, several studies have shown the presence of HPV in malignant breast tumors (29)(30)(31). Conversely, con icting studies were declared the negative relationship between HPV and breast cancer (32)(33)(34).…”

Section: Introductionmentioning

confidence: 99%

Genotype Distribution of Human Papillomavirus (HPV) in Iranian Breast Cancer Patients

et al. 2020

Preprint

Abstract Background: the role of viruses in the aetiology of breast cancer (BC) have been significantly investigated in recent years. Human papillomavirus (HPV) has been detected in invasive breast carcinomas. Most studies have only focused on the detection of viral DNA therefore the aim of this study was to determine the genotypes of each papillomavirus.Methods: we collected and analyzed 70 blocks of Formalin Fixed Paraffin Embedded (FFPE) including 59 BC samples, and 11 noncancerous samples as control from Iranian patients using nested PCR. Real-time PCR supported nested PCR findings. Genotyping of HPV positive samples was performed, the samples were also subjected to a multiplex PCR to detect herpes simplex-1 (HSV-1), herpes simplex-2 (HSV-2), varicella zoster virus (VZV) and cytomegalovirus (CMV) in BC.Results: Papillomavirus DNA was present in 7 of 59 BC samples (11.8%); while none was detected in control samples. The most prevalent type was HPV18, followed by HPV 6, but we didn’t find HPV16 in cases. All HPV positive patients had high tumor grades (II/ III), and the age range of them were from 33 to 73 years (average was about 51). Most of HPV positive patients had low levels of education. Histologic diagnosis of all positive cases were ductal carcinoma. 5 of 59 BC specimens (8.47%), were positive for HSV-1.Conclusions: Our results suggest that high risk HPVs may play a role in breast carcinogenesis. Also we detected HPV6 and HSV1 in our BC samples, taking into account a carcinogenesis role for low risk HPVs, in addition to high risk HPVs. Further large scale studies are warranted to assess the significance of our findings.