Investigation of the response to salinity and to oxidative stress of interspecific potato somatic hybrids grown in a greenhouse

Jbir-Koubaa, Rania; Charfeddine, Safa; Ellouz, Wiem; Saidi, Mohammed Najib; Drira, Noureddine; Gargouri-Bouzid, Radhia; Nouri-Ellouz, Oumèma

doi:10.1007/s11240-014-0648-4

Cited by 73 publications

(44 citation statements)

References 60 publications

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“…Protoplast fusion in other organisms has been applied to combine desired strains based on phenotypic traits such as increased thermal tolerance, increased viral tolerance, and increased antioxidant activity (Jbir‐Koubaa et al. ; Pereira de Carvalho Costa et al. ; Zhang et al.…”

Section: Resultsmentioning

confidence: 99%

Expanding the Symbiodinium (Dinophyceae, Suessiales) Toolkit Through Protoplast Technology

Levin

Suggett

Nitschke

et al. 2017

J Eukaryotic Microbiology

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Dinoflagellates within the genus Symbiodinium are photosymbionts of many tropical reef invertebrates, including corals, making them central to the health of coral reefs. Symbiodinium have therefore gained significant research attention, though studies have been constrained by technical limitations. In particular, the generation of viable cells with their cell walls removed (termed protoplasts) has enabled a wide range of experimental techniques for bacteria, fungi, plants, and algae such as ultrastructure studies, virus infection studies, patch clamping, genetic transformation, and protoplast fusion. However, previous studies have struggled to remove the cell walls from armored dinoflagellates, potentially due to the internal placement of their cell walls. Here, we produce the first Symbiodinium protoplasts from three genetically and physiologically distinct strains via incubation with cellulase and osmotic agents. Digestion of the cell walls was verified by a lack of Calcofluor White fluorescence signal and by cell swelling in hypotonic culture medium. Fused protoplasts were also observed, motivating future investigation into intra- and inter-specific somatic hybridization of Symbiodinium. Following digestion and transfer to regeneration medium, protoplasts remained photosynthetically active, regrew cell walls, regained motility, and entered exponential growth. Generation of Symbiodinium protoplasts opens exciting, new avenues for researching these crucial symbiotic dinoflagellates, including genetic modification.

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Section: Resultsmentioning

confidence: 99%

Expanding the Symbiodinium (Dinophyceae, Suessiales) Toolkit Through Protoplast Technology

Levin

Suggett

Nitschke

et al. 2017

J Eukaryotic Microbiology

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“…They are indispensable for ROS detoxification during stress conditions (Garg and Manchanda 2009). A significant increase in CAT activity in C. arietinum and in S. tuberosum leaves and roots submitted to salinity stress was also described (Eyidogan and Oz 2005;Kukreja et al 2005;Jbir-Koubaa et al 2014).…”

Section: Introductionmentioning

confidence: 86%

“…mulberry (Harinasut et al 2003), Cicer. arietinum (Kukreja et al 2005), Lycopersicon esculentum (Gapinska et al 2008) and Solanum tuberosum (Jbir-Koubaa et al 2014).…”

Section: Introductionmentioning

confidence: 99%

The StDREB1 transcription factor is involved in oxidative stress response and enhances tolerance to salt stress

Bouaziz

Jbir

Charfeddine

et al. 2014

Plant Cell Tiss Organ Cult

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We have shown previously that the potato dehydration responsive element binding (StDREB1) transcription factor plays an important role in regulating and improving salt and drought stress-response genes in potato plant. To further characterize StDREB1 involvement in stress response, we focuse here on the investigation of the StDREB1 target genes by an Electrophoretic mobility shift assay. The data obtains indicated that the StDREB1 protein can bind to both GCC and DRE boxes in the promoter sequence of target genes, suggesting that this transcription factor may play a key role in the response to abiotic-and biotic-stresses by the activation of the DRE-and GCCmediated signaling pathways. In a second step, since some DREB factors were related to the oxidative stress response, we showed that H 2 O 2 treatment led to a significant increase of StDREB1 expression in wild-type potato plants. Moreover, the analysis of the oxidative stress response of StDREB1 transgenic potato plants revealed lower levels of H 2 O 2 and malondialdehyde than wild-type control plants submitted in vitro to salt stress. An increase in the antioxidant enzyme activities including superoxide dismutase (SOD) and catalase was also observed in StDREB1 plants. In addition, an enhanced expression of the Cu/Zn SOD gene was noticed in these StDREB1 transgenic plants, cultivated under salt stress conditions. These results suggest that StDREB1 plays an essential role in the regulation of stress-response by regulating the oxidative stress response. The involvement of this transcription factor in the activation of osmoprotectant synthesis was also confirmed.

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“…al., 2014;Arias-Baldrich et al, 2015). A decrease in this ratio is irrefutable evidence that determine oxidative stress in plants, which concurrently enhances the defensive mechanism in plants by increasing carotenoid contents, as these pigments are effective ROS scavengers Jbir-Koubaa et al, 2015). An increased ratio of (Chla+b/Car) was observed in spring barley, suggesting a decreased carotenoids levels, which in turn resulted in an increased degradation of chlorophylls.…”

Section: Accepted Manuscriptmentioning

confidence: 96%

“…Virtually, all authors agree that an increased level of H 2 O 2 is a stimulus, which immediately increases the activity of POD enzyme, regardless of the cause of stress (Ali et al, 2011;Liu et al, 2013;Anjaneyulu et al, 2014;Biczak, 2016). In contrast to the existing fact that POD has only a regulatory role some of the researchers Jbir-Koubaa et al, 2015) have emphasized that POD has a much higher affinity for H 2 O 2 than for CAT. The importance of POD in H 2 O 2 detoxification can be proved by the observations of Wang et al (2009), who emphasized that POD activity increased whenever plants get affected by stress factors and as long as it is able to carry out its vegetation in these conditions.…”

Section: A C C E P T E D Accepted Manuscriptmentioning

confidence: 99%

Oxidative stress in spring barley and common radish exposed to quaternary ammonium salts with hexafluorophosphate anion

Biczak

Telesiński

Pawłowska

2016

Plant Physiology and Biochemistry

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Investigation of the response to salinity and to oxidative stress of interspecific potato somatic hybrids grown in a greenhouse

Cited by 73 publications

References 60 publications

Expanding the Symbiodinium (Dinophyceae, Suessiales) Toolkit Through Protoplast Technology

Expanding the Symbiodinium (Dinophyceae, Suessiales) Toolkit Through Protoplast Technology

The StDREB1 transcription factor is involved in oxidative stress response and enhances tolerance to salt stress

Oxidative stress in spring barley and common radish exposed to quaternary ammonium salts with hexafluorophosphate anion

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