Investigation of the role of 8-OHdG and oxidative stress in papillary thyroid carcinoma

Abstract

The aim of this study was to determine levels of serum 8-hydroxy-2'-deoxyguanosine (8-OHdG) as an indicator of oxidant-induced DNA damage and oxidant status in patients with papillary thyroid carcinoma before and after surgery. This study included 25 patients with papillary thyroid carcinoma and age-matched 27 healthy controls. Total antioxidant status (TAS), total oxidant status (TOS), lipid hydroperoxide (LOOH), and 8-OHdG levels were measured. 8-OHdG levels were significantly higher in the preoperative papillary thyroid carcinoma (PTC) group compared with the healthy control group (p < 0.001) and were significantly lower after operation in patients with papillary thyroid carcinoma (p = 0.004). Oxidative stress index (OSI) levels were significantly higher in both preoperative and postoperative PTC patients compared with the healthy control group (p < 0.001 and p < 0.001, respectively). TOS levels were higher in the preoperative and postoperative PTC groups compared to the healthy control group (p < 0.001 and p < 0.001, respectively). TAS levels was lower in the preoperative PTC groups compared to the healthy control group (p = 0.011). Serum LOOH levels were higher in both preoperative and postoperative PTC groups compared to the healthy control group (p < 0.001 and p < 0.001, respectively). Correlation analysis yielded that serum 8-OHdG levels were positively correlated with OSİ and LOOH levels in patients with PTC before surgery (r = 0.668, p < 0.001; r = 0.446, p = 0.025, respectively) and had a negative correlation with TAS levels (r = -0.616, p = 0.001). We have shown severe oxidative DNA damage and impaired antioxidant status in papillary thyroid carcinoma.

8-Hydroxy-2’-deoxyguanosine as a biomarker of oxidative stress in acute exacerbation of chronic obstructive pulmonary disease

“…The level of 8-OHdG can be used for estimating oxidative DNA damage. Previous studies have demonstrated that 8-OHdG level increases in many diseases, such as diabetes, cancer, and cardiovascular diseases (15)(16)(17). Our study showed that plasma 8-OHdG levels were elevated in AECOPD patients compared with those in stable COPD and healthy subjects, but no statistical difference was found between stable COPD patients and heathy subjects.…”

“…It is one of the most frequently used biomarkers for evaluating oxidative stress. Previous studies have observed elevated 8-OHdG in diabetes, cancer, cardiovascular diseases, and stable COPD (15)(16)(17). However, the level of 8-OHdG in AECOPD is still unclear.…”

Papillary Thyroid Carcinoma: A Malignant Tumor with Increased Antioxidant Defense Capacity

“…The well developed vascularization of the thyroid clears the thyroid tissue of free radicals and deposits them into circulation, which is the probable reason why previous studies found increased serum oxidant concentrations. In addition, the potential influence of confounding factors for oxidative stress, such as smoking status and co-morbidities, on TOS and TAS values were not evaluated in previous studies (Wang et al 2011;Tabur et al 2015). Based on the present results, we propose that future studies should carry out further characterization of oxidant/ antioxidant status in PTC and BG tissues by assaying the specific enzymatic and non-enzymatic components of the redox system.…”

“…Damaging effect in thyroid tissue occur when there is the regulation misbalance between the produced free radicals and antioxidants, leading to apoptosis via activation of apoptosis signal-regulating kinase 1 (ASK1) (Du et al 2010). Papillary carcinogenesis is associated with increased serum levels of oxidants induced by the DNA damage adduct 8-hydroxy-2′-deoxyguanosine (8-OHdG), which remains high after surgery (Tabur et al 2015). Oxidative stress can be experimentally characterized by assaying numerous enzymatic and non-enzymatic compounds that contribute to the cell's redox status.…”

Exposição ocupacional ao chumbo (Pb): efeitos sobre o estado redox celular e a influência de polimorfismos genéticos sobre a toxicocinética do metal

“…A correlação entre Pb-s e o aumento GST já foi descrita em outros estudos envolvendo exposição ocupacional ao Pb (CONTERATO et al, 2013;DOBRAKOWSKI et al, 2016) Um dos alvos das EROs são os ácidos nucleicos e, por este motivo, os ensaios que avaliam a oxidação de DNA através de 8-OHdG urinária têm sido empregados como ferramentas úteis em estudos de exposição a xenobióticos (CHANG et al, 2011;PRASAD et al, 2013;LI et al, 2014). O interesse crescente na dosagem deste biomarcador se deve à conhecida relação entre a oxidação de DNA e os processos de envelhecimento (SIOMEK et al, 2007;RADAK;BOLDOGH, 2010) e carcinogênese (PYLVÄS et al, 2011;TABUR et al, 2015).…”