Investigation of the role of interleukin-1 receptor antagonist VNTR variant on the Behçet’s disease

Abstract

Objective: Behçet's disease (BD), a chronic multisystem inflammatory disorder, is mainly characterized by relapsing periods of a wide range of clinical symptoms. Several cytokine genes may play important roles in the pathogenesis of BD. Therefore, interleukin-1 receptor antagonist (IL-1Ra) gene 86bp variable number tandem repeat (VNTR) variant was investigated in patients with BD in a Turkish population. Material and methods: One hundred nine patients (60 females, 49 males; the mean age±standard deviation [SD] was 36.56±9.571 years) with BD and one hundred healthy individuals (54 females, 46 males; the mean age±SD was 36.64±2.294 years) were examined in the study. For genotyping, polymerase chain reaction-restriction fragment length polymorphism analysis was employed. Data were analyzed using Statistical Package for Social Sciences (SPSS) 22.0 (p<0.05). Results: The genotype distribution and allele frequencies of the IL-1Ra VNTR variant did not differ significantly between the patients and the controls (p>0.05). The frequency of the a1/a1, a1/a2 genotypes and a1, a2 alleles were the most common both in patients and healthy controls (p=0.37, p=0.26, and p=0.53, respectively). Also, no statistically significant difference was found between the IL-1Ra VNTR variant genotypes and clinical characteristics (p>0.05). Conclusion:The results of this study do not support an association between the IL-1Ra VNTR variant and the risk of BD in a Turkish population. However, further studies of this variant with larger sample sizes and different ethnicities are required for confirmation.

Interleukin-1 gene (IL-1) polymorphism in patients with Behçet’s Disease, and its relationship with disease manifestations

“…However, in another study, the IL-1Ra VNTR variant was not related to the risk of BD in a Turkish population. 20 The mutant IL1RN-VNTR polymorphism was seen in patients with CNS involvement, whereas wild-type was more often seen in patients without CNS involvement.…”

“…However, in another study, the IL-1Ra VNTR variant was not related to the risk of BD in a Turkish population. 20 The mutant IL1RN-VNTR polymorphism was seen in patients with CNS involvement, whereas wild-type was more often seen in patients without CNS involvement. IL-1β as a member of the IL-1 cytokine family, is also related to gastric acid secretion, amplifies inflammation, and reveals possible gastric tumorigenesis; literature involves many studies searching IL-1B-3954C > T polymorphism in patients with gastric cancer and/or precancerous gastric lesions.…”

Correlation of Interleukin-1 Receptor Antagonist (IL-1RA) gene polymorphisms in various cancers

“…Gels were run in 1X Tris Borate EDTA (TBE) buffers. Stocks of TBE (10X), sample loading (6X) and ethidium bromide which is 10 mg/ml were prepared as here and described well [13].…”

IL1RN VNTR Polymorphism and kidney damage in sickle cell anemia patients

“…The amplicon sizes were determined by comparing them against a 100 bp ladder. The PCR amplicon sizes were recorded and converted into alleles as described elsewhere (19).…”