Investigation of the Toxic Principles of Hippomane mancinella L. III.**College of Pharmacy, University of Florida, Gainesville

Euphorbiaceae Juss.

“…An aqueous extract of the wood yields a somewhat thermolabile toxin, which, when administered parenterally, causes a drop in blood pressure and an increase in salivation; these effects are antagonized by epinephrine and atropine, respectively (Schaeffer et al 1954;Carroll et al 1957). An aqueous extract of the wood yields a somewhat thermolabile toxin, which, when administered parenterally, causes a drop in blood pressure and an increase in salivation; these effects are antagonized by epinephrine and atropine, respectively (Schaeffer et al 1954;Carroll et al 1957).…”

On the Active Principles of the Spurge Family, X. 1,2Skin Irritants, Cocarcinogens, and Cryptic Cocarcinogens from the Latex of the Manchineel Tree

“…Transesterification of ' , affording factor group Aly (6,7) and fatty acid methyl esters.-To 20 mg of the cryptic factor group M'y 0.01 M NaOCH3-MeOH (8 ml) was added. Seven hours later the reaction was stopped, and the reaction products were separated by tic in Et20-petroleum ether-Me2CO (1:1:1).…”

“…Factor group My (6,7).-ms m/z 578 (M + ) and 552 (M+), 328, 310, 233, 207; ir (CH2C12) 3600, 3550, 3430 (OH); 1705 (CO); 1660, 1630 cm"1 (C=C); uv (MeOH) X max 232, 259, 269, 280, sh 320; pmr 1-H: 7.6 (m), 16-H2: 5.04 (s), 4.91 (s, br. ); 14-H: 4.38 (dj=2.5 Hz); 20-H2: 4.09 (s); 8-H, 10-H: 3.2 (m); 17-H3, 19-H3: 1.8 (m); 18-H3: 1.15 (d,J=7 Hz); OH (exchangeable with D20): 2.4 ppm; acid moieties: 1 olefinic H: 6.73 (dd,J; = 10 Hz,72= 16 Hz); appr.…”

“…Several attempts were made to investigate the toxic principles of H. mancinella (5)(6)(7)(8)(9). Obviously more than one toxic principle is present because response to ingestion of the fruits seems not exactly comparable with that of contact with the latex (2).…”

“…2Part of the present work was presented at the International Congress for Research on Medicinal Plants, Munich, September [6][7][8][9][10]1976; see also loc.cit. 17 besides several known plant constituents, an alkaloid resembling physostigmine (6) and a "crystalline tannin" (hippomanin A) (8,9) have been isolated from leaves, wood, or fruits.…”