Investigation of the Valorization of Methanolic Extract of <i>Punica granatum</i> L. Peel in Terms of Phytochemical, Trace Element, Antioxidant Activities and ADMET Profile of Active Compounds

Abstract

In this study, to valorize the pomegranate peels, the phytochemical composition of the methanolic extract of Punica granatum L. peel (PPME) was characterized by high-resolution liquid chromatographymass spectrometry (LC/HRMS), while the mineral composition of the extract was determined by ICP AES. In the methanolic pomegranate peel extract, thirty-five different compounds were identified by LC/HRMS, and it was determined that these compounds were mainly terpenoids, lipids and flavonoids. Total polyphenol, flavonoid, and tannins contents of the PPME extract were determined as 211.43 mg GAE/g polyphenol, 20.7 mg QE/g flavonoids and 11.5 mg TEA/g tannins. In order to determine the antioxidant capacity of the extract, we studied its 1,1-diphenyl-2-picryl-hydrazyl radicals (DPPH . ) and reducing power activity assays. These colorimetric assays showed that PPME has strong reducing power and anti-radical capacity against DPPH with the IC50 values 0.210±0.005 mg/mL and 0.3±0.0045 mg/mL,