Investigation of therapeutic effects in the wound healing of chitosan/pGM-CSF complexes

Alan, Saadet; Şalva, Emine; Karakoyun, Berna; Çakalağaoğlu, Fulya; Özbaş, Suna; Akbuğa, Jülide

doi:10.1590/s2175-97902022e19668

Cited by 5 publications

(8 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…I1, I3, and I5 formulations loaded with ampicillin showed a lower swelling capacity. The high values of the swelling index indicate that water molecules can easily penetrate the polymer . This decrease could be because the pores were filled with drugs and the water intake was hindered.…”

Section: Resultsmentioning

confidence: 99%

“…The swelling property is a critical parameter in the formulation of films. The swelling index is a metric that describes the swelling properties of film when it comes into contact with an aqueous media . Chitosan films were generated using the casting method, and Figure illustrates the quality of the produced chitosan films.…”

Section: Resultsmentioning

confidence: 99%

“…Thickness homogeneity is an essential physical feature in oral film evaluation because it significantly impacts the precision of film dosage distribution. 35 3.2. MD Simulations.…”

Section: Formulation Development and Characterizationsmentioning

confidence: 99%

“…The swelling index is a metric that describes the swelling properties of film when it comes into contact with an aqueous media. 35 Chitosan films were generated using the casting method, and Figure 1 illustrates the quality of the produced chitosan films. According to Figure 1, the swelling index of the films increased substantially at first, and the chitosan films appeared to be entirely swelled and hydrated after 15 min.…”

Section: Formulation Development and Characterizationsmentioning

confidence: 99%

“…The high values of the swelling index indicate that water molecules can easily penetrate the polymer. 35 This decrease could be because the pores were filled with drugs and the water intake was hindered. A discussion of these results in the light of MD studies is presented in Section 3.2.3.…”

Section: Formulation Development and Characterizationsmentioning

confidence: 99%

See 4 more Smart Citations

Development, Evaluation, and Molecular Dynamics Study of Ampicillin-Loaded Chitosan–Hyaluronic Acid Films as a Drug Delivery System

Arısoy,

Bux,

Herwig

et al. 2024

ACS Omega

Self Cite

View full text Add to dashboard Cite

Periodontitis is an inflammatory periodontal disease defined by the progressive loss of tissues surrounding the tooth. Ampicillin is an antibiotic for managing and treating specific bacterial infections, including periodontitis. Periodontal pockets occur due to periodontal disease progression and act as a natural reservoir that is easily reachable for the insertion of a delivery system, and the amount of drug to be released has a major role in the efficiency of treatment of the disease. Polyelectrolyte complexes (PECs), particularly those based on chitosan and hyaluronic acid combinations, offer a promising avenue to overcome the challenges associated with drug delivery. These complexes are both biodegradable and biocompatible, making them an optimal choice for enabling targeted drug delivery. This study centers on developing and assessing the structure and dynamic attributes of a drug−PEC system encompassing ampicillin and chitosan−hyaluronic acid components, which represents a targeted drug delivery system to better alleviate the periodontitis. To achieve this goal, we conducted experiments including weight and drug content uniformity, swelling ındex, drug release %, FT-IR and SEM analyses, and atomistic molecular dynamics simulations on the drug PECs loaded with ampicillin with varying amounts of hyaluronic acid. All simulations and the experimental analysis suggested that increased HA amount resulted in an increase in drug release % and swelling index. The simulation outcomes provide insights into the nature of the drug and PEC interactions alongside transport properties such as drug diffusion coefficients. These coefficients offer valuable insights into the molecular behavior of ampicillin−PEC drug delivery systems, particularly in the context of their application in periodontitis treatment.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

“…Thickness homogeneity is an essential physical feature in oral film evaluation because it significantly impacts the precision of film dosage distribution. 35 3.2. MD Simulations.…”

Section: Formulation Development and Characterizationsmentioning

confidence: 99%

Section: Formulation Development and Characterizationsmentioning

confidence: 99%

Section: Formulation Development and Characterizationsmentioning

confidence: 99%

See 3 more Smart Citations

Development, Evaluation, and Molecular Dynamics Study of Ampicillin-Loaded Chitosan–Hyaluronic Acid Films as a Drug Delivery System

Arısoy,

Bux,

Herwig

et al. 2024

ACS Omega

Self Cite

View full text Add to dashboard Cite

show abstract

Preparation and in vitro characterization of curcumin loaded Chitosan-Hyaluronic acid polyelectrolyte complex based hydrogels

Arısoy,

Şalva

2023

Drug Development and Industrial Pharmacy

View full text Add to dashboard Cite

Chitosan-Based Dressing as a Sustained Delivery System for Bioactive Cytokines

Lewicki,

Zwoliński,

Hovagimyan

et al. 2023

IJMS

View full text Add to dashboard Cite

Wounds represent a common occurrence in human life. Consequently, scientific investigations are underway to advance wound healing methodologies, with a notable focus on dressings imbued with biologically active compounds capable of orchestrating the wound microenvironment through meticulously regulated release mechanisms. Among these bioactive agents are cytokines, which, when administered to the wound milieu without appropriate protection, undergo rapid loss of their functional attributes. Within the context of this research, we present a method for fabricating dressings enriched with G-CSF (granulocyte colony-stimulating factor) or GM-CSF (granulocyte-macrophage colony-stimulating factor), showcasing both biological activity and protracted release dynamics. Based on Ligasano, a commercial polyurethane foam dressing, and chitosan crosslinked with TPP (sodium tripolyphosphate), these dressings are noncytotoxic and enable cytokine incorporation. The recovery of cytokines from dressings varied based on the dressing preparation and storage techniques (without modification, drying, freeze-drying followed by storage at 4 °C or freeze-drying followed by storage at 24 °C) and cytokine type. Generally, drying reduced cytokine levels and their bioactivity, especially with G-CSF. The recovery of G-CSF from unmodified dressings was lower compared to GM-CSF (60% vs. 80%). In summary, our freeze-drying approach enables the storage of G-CSF or GM-CSF enriched dressings at 24 °C with minimal cytokine loss, preserving their biological activity and thus enhancing future clinical availability.

show abstract

Investigation of therapeutic effects in the wound healing of chitosan/pGM-CSF complexes

Cited by 5 publications

References 35 publications

Development, Evaluation, and Molecular Dynamics Study of Ampicillin-Loaded Chitosan–Hyaluronic Acid Films as a Drug Delivery System

Development, Evaluation, and Molecular Dynamics Study of Ampicillin-Loaded Chitosan–Hyaluronic Acid Films as a Drug Delivery System

Preparation and in vitro characterization of curcumin loaded Chitosan-Hyaluronic acid polyelectrolyte complex based hydrogels

Chitosan-Based Dressing as a Sustained Delivery System for Bioactive Cytokines

Contact Info

Product

Resources

About