Investigation of toxic effects of amorphous SiO2 nanoparticles on motility and oxidative stress markers in rainbow trout sperm cells

Özgür, Mustafa Erkan; Ulu, Ahmet; Özcan, İmren; Balcıoğlu, Sevgi; Ateş, Burhan; Köytepe, Süleyman

doi:10.1007/s11356-019-04941-5

Cited by 14 publications

(5 citation statements)

References 40 publications

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“…The present results are similar to the study from Xu et al (22), who found that SiNPs could cause reversible damages to the sperms and testicular structures. The apoptosis of the sperm cells induced by the SiNPs was also previously reported by Özgür ME (16,23).…”

Section: Discussionsupporting

confidence: 78%

Silica nanoparticles inducing the apoptosis of spermatocyte cell through microRNA-450b-3p targeting MTCH2-mediating mitochondrial signaling pathway

Zhou

Liu

et al. 2020

Preprint

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Background: Silica nanoparticles (SiNPs) are found in environmental particulate matter and are proven to have adverse effects on fertility. The relationship and underlying mechanisms between miRNAs and apoptosis induced by SiNPs during spermatogenesis is currently ambiguous. Experimental design: The present study was designed to investigate the role of miRNA-450b-3p in the reproductive toxicity caused by SiNPs. In vivo, 40 male mice were randomly divided into control and SiNPs groups, 20 per group. The mice in the SiNPs group were administrated 20 mg/kg SiNPs by tracheal perfusion once every 5 days, for 35 days, and the control group were given the equivalent of a normal luminal saline. In vitro, spermatocyte cells were divided into 0 and 5 μg/mL SiNPs groups, after passaged for 30 generations, the GC-2spd cells in 5 μg/mL SiNPs groups were transfected with miRNA-450b-3p and its mimic and inhibitor. Results: In vivo, the results showed that SiNPs damaged tissue structures of testis, decreased the quantity and quality of the sperm, reduced the expression of miR-450b-3p, and increased the protein expressions of the MTCH2, BID, BAX, Cytochrome C, Caspase-9, and Caspase-3 in the testis. In vitro, SiNPs obviously repressed the viability and increased the LDH level and apoptosis rate, decreased the levels of the miR-450b-3p, significantly enhanced the protein expressions of the MTCH2, BID, BAX, Cytochrome C, Caspase-9, Caspase-3; while the mimic of miR-450b-3p reversed the changes induced by SiNPs, but inhibitor further promoted the effects induced by SiNPs.Conclusion: The result suggested that SiNPs could induce the spermatocyte apoptosis by inhibiting the miR-450b-3p expression to target promoting the MTCH2 resulting in activating mitochondrial apoptotic signaling pathways in the spermatocyte cells.

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Section: Discussionsupporting

confidence: 78%

Silica nanoparticles inducing the apoptosis of spermatocyte cell through microRNA-450b-3p targeting MTCH2-mediating mitochondrial signaling pathway

Zhou

Liu

et al. 2020

Preprint

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“…In study by Ozgur et al [45][46][47][48]62] the ALH was not affected by nanoproducts while the BCF increased in the highest concentrations of nanoproducts (when the short incubation time is considered). In our study, the factors tested also had no effect on ALH, while BCF increased and then decreased with increasing concentration of nanometals and extended incubation time.…”

Section: Discussionmentioning

confidence: 96%

“…The highest LIN occurred at 0.1 mg·L −1 . In the Ozgur et al study [ 62 ], incubation with SiO 2 decreased LIN. In our study after 2–24 h of incubation, LIN values were significantly or slightly positively influenced by copper nanoparticles and low concentrations of ionic form.…”

Section: Discussionmentioning

confidence: 97%

“…Based on the results from Ozgur et al [ 47 ], there was a statistically significant reduction in sperm velocity (VCL) after applying 50 mg·L −1 of Fe 3 O 4 NPs. The same concentration of silica nanoparticles (SiO 2 NPs) increased VCL [ 62 ]. In the Ozgur et al [ 46 ] paper, incubation with TiO 2 NPs for 3 h had no significant effect on VCL while decreasing VAP occurred at concentrations equal to or greater than 10 mg·L −1 of TiO 2 .…”

Section: Discussionmentioning

confidence: 99%

“…In the studies by Ozgur et al [ 45 , 46 , 47 , 48 , 62 ], the percentage of motile sperm was not analysed. In the present study where rainbow trout spermatozoa were incubated with copper nanoproducts, lesser toxicity of CuO NPs than Cu NPs was seen.…”

Section: Discussionmentioning

confidence: 99%

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The Effect of Copper and Copper Oxide Nanoparticles on Rainbow Trout (Oncorhynchus mykiss W.) Spermatozoa Motility after Incubation with Contaminants

Garncarek

Dziewulska

Kowalska‐Góralska

2022

IJERPH

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The study aimed to analyse the effect of copper nanoparticles of similar particle size of Cu and CuO and copper ions (CuSO4) on the motility parameters of rainbow trout spermatozoa after long-term exposure and compare its harmful effect. Nanoproducts of Cu and CuO (Cu NPs, CuO NPs) of primary particle size around 50 nm and ionic solution of CuSO4 were used for the study. Suspension of concentrations 0, 1, 5, 10, 25, and 50 mg Cu·L−1 of Cu NPs, CuO NPs, and CuSO4 was dissolved in an artificial seminal plasma. Milt was mixed with the prepared solution and stored in a fridge, at 6 °C, for 96 h. At the defined incubation time, spermatozoa were activated for movement, and six motility parameters were evaluated using an automated system (CASA). Increasing concentrations of Cu NPs, CuO NPs, and CuSO4 in an incubation medium in parallel decreased the percentage of motile sperm (MOT). The effect of Cu NPs and ionic copper on MOT was more deleterious than that of CuO NPs. Copper products slightly increased the velocity (VCL) compared to the control, particularly up to 24 h of storage. Linearity (LIN) was improved by three tested products, particularly CuO NPs. Generally, the motility duration was prolonged when the sperm was incubated with copper products compared to the control. Nanoproducts made from different compounds of the same elements of similar particle size have a different effect on cells. Cu NPs were more harmful than CuO NPs. The effect of Cu NPs was similar to an ionic form of CuSO4. When incubated, the copper nanoproducts and ionic form exert a slightly positive effect on spermatozoa velocity, linearity, and motility duration, particularly up to 24 h of storage.

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Tire Rubber Based Microplastic Particles Cause Adverse on Quality Parameters of Rainbow Trout Sperm Cells

Özgür,

Ulu,

Sezer

et al. 2024

Bull Environ Contam Toxicol

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Investigation of toxic effects of amorphous SiO2 nanoparticles on motility and oxidative stress markers in rainbow trout sperm cells

Cited by 14 publications

References 40 publications

Silica nanoparticles inducing the apoptosis of spermatocyte cell through microRNA-450b-3p targeting MTCH2-mediating mitochondrial signaling pathway

Silica nanoparticles inducing the apoptosis of spermatocyte cell through microRNA-450b-3p targeting MTCH2-mediating mitochondrial signaling pathway

The Effect of Copper and Copper Oxide Nanoparticles on Rainbow Trout (Oncorhynchus mykiss W.) Spermatozoa Motility after Incubation with Contaminants

Tire Rubber Based Microplastic Particles Cause Adverse on Quality Parameters of Rainbow Trout Sperm Cells

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