Investigation of Vascular Endothelial Growth Factor Gene Polymorphisms and Its Association with Clinicopathologic Characteristics in Gastric Cancer

Abstract

Objective: Vascular endothelial growth factor (VEGF) is known to be a potent proangiogenic factor. This study evaluates the potential association of three VEGF gene polymorphisms (–460T > C, +405G > C, and 936C > T) with the susceptibility to and clinicopathologic characteristics of gastric cancer. Methods: The VEGF genotypes were determined using paraffin-embedded tissue from 413 patients who underwent a surgical resection and peripheral blood lymphocytes from 413 healthy controls by PCR-RFLP assay. Results: There was no difference in the allele frequency of –460T > C polymorphism. However, for the +405G > C polymorphism, the +405C allele was associated with a significantly decreased susceptibility to gastric cancer [odds ratio (OR) 0.686; 95% confidence interval (CI) 0.564–0.834]. Although there was no significant difference in the distribution of the 936C > T polymorphism between the two groups, the 936T allele was associated with a decreased susceptibility to gastric cancer (OR 0.757; 95% CI 0.591–0.970). In the haplotype analyses, the haplotype TCT (OR 0.405; 95% CI 0.263–0.624) was most closely associated with a decreased susceptibility to gastric cancer. However, no significant association was observed between the frequency of the genotypes or alleles and the clinicopathologic characteristics of gastric cancer. Conclusion: These observations imply that the VEGF gene polymorphisms may be associated with the susceptibility to gastric cancer. However, further studies of other VEGF sequence variants and their biological functions are needed to understand the role of the VEGF polymorphisms in determining the susceptibility to gastric cancer.

The Role of Vascular Endothelial Growth Factor Genetic Variability in Cancer

“…These studies include those that evaluate cancer risk and prognosis (25,(27)(28)(29)(30)(31)(32)(33)(34)(35)(36), retinopathy (20,(37)(38)(39)(40)(41)(42), nephropathy (43)(44)(45)(46), pre-eclampsia (47,48), and vasculopathy (49-51), among many others. Many of these studies show an increased association of disease and/or inferior outcomes in the subgroup with genotype that would predict a higher VEGF expression.…”

Vascular endothelial growth factor +936C/T polymorphism and cancer risk in Asians: a meta-analysis

“…In total, 21 studies (Abe et al, 2002;Kataoka et al, 2006;Hsiao et al, 2007;Amano et al, 2008;Bae et al, 2008a,b;Chae et al, 2006Chae et al, , 2008Cheng et al, 2008;Ke et al, 2008;Hsing et al, 2008;Al-Moundhri et al, 2009;Tahara et al, 2009;Wu et al, 2009;Li et al, 2010;Kim et al, 2010;Bao et al, 2011;Beeghly-Fadiel et al, 2011;Kang et al, 2011;Zhou et al, 2011) comprising 8298 cancer cases and 8053 controls were included in the meta-analysis. The VEGF allele +936T frequency was 17.78% in cases and 17.84% in controls.…”

“…According to the subgroup analyses by cancer type, there were no significant associations between the VEGF allele +936C/T polymorphism and risk of colorectal cancer and gastric cancer in different genetic comparisons. Chae et al (2006) were combined in analyses, since the articles share the same control subjects.…”

Role of +405C>G and +936C>T Polymorphisms of the Vascular Endothelial Growth Factor Gene and Risk of Esophageal Cancer in the Kashmiri Population

“…Multiple single nucleotide polymorphisms (SNP) have been identified within the VEGF gene, +405G/C and +936C/T are in linkage disequilibrium and are of interest due to their potential functional implications. Investigated, prospectively, in a large cohort of patients with esophageal cancer by evaluating the association of each SNP independently, (Chae et al, 2006).…”