The low-frequency in-plane thickness noise generating from the displacement of air by rotating blades has an important influence on helicopter detection. An on-blade control technique to reduce thickness noise is developed in this paper based on the principle of sound field cancellation. Following the theoretical study on the mechanism of thickness noise reduction using in-plane unsteady force, a 2-m diameter rotor with an active trailing-edge winglet are designed and tested in a fully anechoic chamber. The winglet installed on the outboard blade is used to generate the unsteady force and anti-noise to counteract the thickness noise. The results demonstrate that effective reduction of thickness noise up to 3 dB is achieved in the front of the rotor when the winglet is under the one-harmonic control with 3 ° of deflection angle. Moreover, the experiments of frequency, amplitude, and phase scanning are carried out to study the parametric effects of winglet motions on noise reduction. The ability of noise reduction is proportional to the deflection amplitude of the winglet in each frequency. The control phase determines where noise can be reduced. There is an optimal phase angle at each frequency to minimize the noise at the observations, and it varies with different frequencies. The relationship among observation position, control phase, and frequency is derived, and the approximate expression of the optimal phase is presented.