Investigation on Pathological Aspects, Mode of Transmission, and Tissue Tropism of <i>Antheraea proylei</i> Nucleopolyhedrovirus Infecting Oak Tasar Silkworm

Khajje, Diksha; Devi, Sinam Subharani; Subrahmanyam, Gangavarapu; Kobayashi, Jun; Sivaprasad, V.; Terenius, Olle; Ponnuvel, Kangayam M.

doi:10.1093/jisesa/ieac057

Cited by 2 publications

(4 citation statements)

References 18 publications

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“…The visible symptoms of AnprNPV infection were observed post 7 days of infection showing retarded growth and black bands/stripes across the body of the silkworm. These results are in accordance with earlier reported observations in which the symptoms led to the death of the larvae (Khajje et al, 2022). This information would help the sericulture farmers to control the disease by discarding the infected larvae/batches and applying disinfectants and taking other prophylactic measures for better control of disease.…”

Section: Resultssupporting

confidence: 92%

“…Baculoviruses generally display a horizontal mode of transmission, mainly through ingestion of occlusion bodies (OBs); however, AnprNPV shows a trans-ovum vertical mode of transmission in addition to a horizontal mode (Khajje et al, 2022). Early detection of the virus aids in the prevention of disease spread and substantial crop losses.…”

Section: Resultsmentioning

confidence: 99%

“…The bioassay studies were conducted with AnprNPV as described by Khajje et al, 2022. A batch of uninfected larva was used as a control.…”

Section: Collection and Validation Of Disease-infected Oak Tasar Silk...mentioning

confidence: 99%

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Development of a Molecular Diagnostic Technique for the Detection of Baculovirus Infecting Oak Tasar Silkworm, Antheraea Proylei

Khajje¹,

Subrahmanyam²,

Ponnuvel³

2023

AJMBES

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Antheraea proylei, a temperate oak tasar silkworm, is one of the economically important silkworms reared for the production of oak tasar silk. A. proylei is frequently infected with A. proylei nucleopolyhedrovirus (AnprNPV), which causes tiger band disease leading to severe economic loss in oak tasar silk production. Therefore, development of an accurate diagnostic tool may facilitate early detection of pathogen, thus preventing massive economic loss. In the current study, we have developed a real-time quantitative polymerase chain reaction (RT-qPCR) for diagnosis of AnprNPV. The primers specific to p94 gene of AnprNPV were designed to investigate their sensitivity and specificity. The AnprNPV detection limit in RT-qPCR was found to be 2.5 × 101 copy number with 98.12% efficiency. The developed diagnostic technique is 100 times more sensitive than the conventional PCR for the detection of AnprNPV. Further, the technique was validated with filed samples wherein the AnprNPV viral loads in the oak tasar silkworms were ranged from 103 to 1010 copies/l.

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Section: Resultssupporting

confidence: 92%

Section: Resultsmentioning

confidence: 99%

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Development of a Molecular Diagnostic Technique for the Detection of Baculovirus Infecting Oak Tasar Silkworm, Antheraea Proylei

Khajje¹,

Subrahmanyam²,

Ponnuvel³

2023

AJMBES

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“…Quantitative PCR has been used in sericulture for various purposes, including the detection and quantification of pathogens, and gene expression studies (Khajje et al., 2022). qPCR was used to understand the critical pathology of B. mori bidensovirus (BmBDV).…”

Section: Molecular Tools For the Detection Of Silkworm Pathogensmentioning

confidence: 99%

Molecular diagnostics in sericulture: A paradigm shift towards disease diagnosis in silkworms

Deepika,

Ramesh,

Kumar

et al. 2024

Entomologia Exp Applicata

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Sericulture, the practice of rearing silkworms for the production of silk, is an essential agro‐based industry in several countries. However, silkworms are susceptible to a variety of diseases caused by viruses, bacteria and parasites, which may have a significant negative impact on global silk production. Traditional methods of pathogen identification, such as microscopy and laboratory culturing, have limitations in terms of accuracy and efficiency. The development of molecular techniques for pathogen identification has revolutionised the field of sericulture over the last decade. Genomic DNA and RNA‐based molecular techniques allow for the rapid and accurate detection of disease‐causing pathogens in silkworms. Molecular diagnosis has several advantages over traditional methods, including increased sensitivity and specificity, shorter turnaround time and the ability to detect pathogens that are difficult to culture or visualise under a microscope. Molecular techniques have been applied to detect several important pathogens of silkworms, including Nosema sp., nucleopolyhedrovirus, cypovirus, iflavirus and bidensovirus. The use of molecular diagnostics in sericulture is immensely important as the demand for high‐quality silk increases globally and the assessment of emerging pathogens associated with crop loss is essential. Major advancements in the improvement and application of molecular methods for diagnosing widespread silkworm pathogens are discussed.

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Investigation on Pathological Aspects, Mode of Transmission, and Tissue Tropism of Antheraea proylei Nucleopolyhedrovirus Infecting Oak Tasar Silkworm

Cited by 2 publications

References 18 publications

Development of a Molecular Diagnostic Technique for the Detection of Baculovirus Infecting Oak Tasar Silkworm, Antheraea Proylei

Development of a Molecular Diagnostic Technique for the Detection of Baculovirus Infecting Oak Tasar Silkworm, Antheraea Proylei

Molecular diagnostics in sericulture: A paradigm shift towards disease diagnosis in silkworms

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