Heat transfer rate of TiO 2 and water Nano fluids were measured in a shell and tube heat exchanger under the turbulent flow condition. Even with insignificant focus of Nano particles in the base fluids, results in enhancement of heat transfer coefficient. Heat transfer rate of TiO 2, at its optimum Nano particle concentration are greater than the water particle. From the past research, it is evident that, the addition of Nano particles increases the heat transfer rate.So for the present study, TiO 2 Nanoparticles were chosen for dispersing them in the water, for enhancing heat transfer properties and comparing them with only pure base fluid. The current study is also extended to find the overall heat transfer coefficient and the amount of heat transfer enhancement at different flow rates. Further, heat transfer characteristics like Reynolds number and effectiveness were also measured. From the experimental outcomes, it is observed that, there is a clear enhancement in the heat transfer characteristics with addition of Nano particle, at different volume concentrations (0.05%, 0.1%, 0.15% and 0.2%), for different flow rates.