Investigation on the Antifungal Ingredients of Saccharothrix Yanglingensis Hhs.015, an Antagonistic Endophytic Actinomycete Isolated from Cucumber Plant

Wang, Hua; Tian, Runze; Tian, Qizhen; Xia, Yan; Huang, Lili; Ji, Zhiqin

doi:10.3390/molecules24203686

Cited by 11 publications

(3 citation statements)

References 26 publications

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“…The compounds produced by endophytic microorganisms have been showing promise as a more environmentally friendly alternative ( Wang et al, 2019 ). We reported here the capacity of 12 endophytic fungi isolated from tissues of the medicinal plants V. divergens and S. adstringens to produce secondary metabolites with biological activities against phytopathogenic fungi and bacteria.…”

Section: Discussionmentioning

confidence: 99%

Endophytes of Brazilian Medicinal Plants With Activity Against Phytopathogens

et al. 2021

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Plant diseases caused by phytopathogens are responsible for significant crop losses worldwide. Resistance induction and biological control have been exploited in agriculture due to their enormous potential. In this study, we investigated the antimicrobial potential of endophytic fungi of leaves and petioles of medicinal plants Vochysia divergens and Stryphnodendron adstringens located in two regions of high diversity in Brazil, Pantanal, and Cerrado, respectively. We recovered 1,304 fungal isolates and based on the characteristics of the culture, were assigned to 159 phenotypes. One isolate was selected as representative of each phenotype and studied for antimicrobial activity against phytopathogens. Isolates with better biological activities were identified based on DNA sequences and phylogenetic analyzes. Among the 159 representative isolates, extracts from 12 endophytes that inhibited the mycelial growth (IG) of Colletotrichum abscissum (≥40%) were selected to expand the antimicrobial analysis. The minimum inhibitory concentrations (MIC) of the extracts were determined against citrus pathogens, C. abscissum, Phyllosticta citricarpa and Xanthomonas citri subsp. citri and the maize pathogen Fusarium graminearum. The highest activity against C. abscissum were from extracts of Pseudofusicoccum stromaticum CMRP4328 (IG: 83% and MIC: 40 μg/mL) and Diaporthe vochysiae CMRP4322 (IG: 75% and MIC: 1 μg/mL), both extracts also inhibited the development of post-bloom fruit drop symptoms in citrus flowers. The extracts were promising in inhibiting the mycelial growth of P. citricarpa and reducing the production of pycnidia in citrus leaves. Among the isolates that showed activity, the genus Diaporthe was the most common, including the new species D. cerradensis described in this study. In addition, high performance liquid chromatography, UV detection, and mass spectrometry and thin layer chromatography analyzes of extracts produced by endophytes that showed high activity, indicated D. vochysiae CMRP4322 and P. stromaticum CMRP4328 as promising strains that produce new bioactive natural products. We report here the capacity of endophytic fungi of medicinal plants to produce secondary metabolites with biological activities against phytopathogenic fungi and bacteria. The description of the new species D. cerradensis, reinforces the ability of medicinal plants found in Brazil to host a diverse group of fungi with biotechnological potential.

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Section: Discussionmentioning

confidence: 99%

Endophytes of Brazilian Medicinal Plants With Activity Against Phytopathogens

et al. 2021

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“…Furthermore, many metabolites have been discovered with antimicrobial properties similar to phytopathogenic fungi, such as amphotericin B, macrolides, actinomycin D, natamycin, antimycin, and neopeptine [ 36 , 37 , 38 ]. Macrolides are a group of antibiotics produced by actinomycetes that inhibit fungus protein synthesis [ 39 ].…”

Section: Main Actinomycete Antagonistic Mechanisms To Phytopathogenic...mentioning

confidence: 99%

Actinomycete Potential as Biocontrol Agent of Phytopathogenic Fungi: Mechanisms, Source, and Applications

Torres-Rodríguez¹,

Pérez

Quiñones-Aguilar³

et al. 2022

Plants

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Synthetic fungicides have been the main control of phytopathogenic fungi. However, they cause harm to humans, animals, and the environment, as well as generating resistance in phytopathogenic fungi. In the last few decades, the use of microorganisms as biocontrol agents of phytopathogenic fungi has been an alternative to synthetic fungicide application. Actinomycetes isolated from terrestrial, marine, wetland, saline, and endophyte environments have been used for phytopathogenic fungus biocontrol. At present, there is a need for searching new secondary compounds and metabolites of different isolation sources of actinomycetes; however, little information is available on those isolated from other environments as biocontrol agents in agriculture. Therefore, the objective of this review is to compare the antifungal activity and the main mechanisms of action in actinomycetes isolated from different environments and to describe recent achievements of their application in agriculture. Although actinomycetes have potential as biocontrol agents of phytopathogenic fungi, few studies of actinomycetes are available of those from marine, saline, and wetland environments, which have equal or greater potential as biocontrol agents than isolates of actinomycetes from terrestrial environments.

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“…Actinomycetes are a unique group within the bacterial domain, capable of producing a variety of bioactive substances that have important practical and economic uses, but little research has been done on elicitors. Saccharothrix yanglingensis Hhs.015 is an actinomycete isolated from cucumber roots that can effectively control plant pathogens through competition, secretion of antagonistic factors, and induced resistance (Fan et al, 2016; Lu et al, 2018; Wang et al, 2019). Hhs.015 has shown great efficacy against apple Valsa canker, Sclerotinia diseases of rapeseed, and tomato leaf mould in field tests (Chen et al, 2013; Li et al, 2016; Min et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

A novel protein elicitor (PeSy1) from Saccharothrix yanglingensis induces plant resistance and interacts with a receptor‐like cytoplasmic kinase in Nicotiana benthamiana

Wang

Liu

Ren

et al. 2023

Molecular Plant Pathology

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Previously, we reported a rare actinomycete Saccharothrix yanglingensis Hhs.015 with strong biocontrol ability, which can colonize plant tissues and induce resistance, but the key elicitor and immune mechanisms were unclear. In this study, a novel protein elicitor screened from the genome of Hhs.015, PeSy1 (protein elicitor of S. yanglingensis 1), could induce a strong hypersensitive response (HR) and resistance in plants. The PeSy1 gene encodes an 11 kDa protein with 109 amino acids that is conserved in Saccharothrix species. PeSy1‐His recombinant protein induced early defence events such as a cellular reactive oxygen species burst, callose deposition, and the activation of defence hormone signalling pathways, which enhanced Nicotiana benthamiana resistance to Sclerotinia sclerotiorum and Phytophthora capsici , and Solanum lycopersicum resistance to Pseudomonas syringae pv. tomato DC3000. Through pull‐down and mass spectrometry, candidate proteins that interacted with PeSy1 were obtained from N. benthamiana . We confirmed the interaction between receptor‐like cytoplasmic kinase RSy1 (Response to PeSy1) and PeSy1 using co‐immunoprecipitation, bimolecular fluorescence complementation, and microscale thermophoresis. PeSy1 treatment promoted up‐regulation of marker genes in pattern‐triggered immunity. The cell death it elicited was dependent on the co‐receptors NbBAK1 and NbSOBIR1, suggesting that PeSy1 acts as a microbe‐associated molecular pattern from Hhs.015. Additionally, RSy1 positively regulated PeSy1‐induced plants resistant to S. sclerotiorum . In conclusion, our results demonstrated a novel receptor‐like cytoplasmic kinase in the plant perception of microbe‐associated molecular patterns, and the potential of PeSy1 in induced resistance provided a new strategy for biological control of actinomycetes in agricultural diseases.

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Investigation on the Antifungal Ingredients of Saccharothrix Yanglingensis Hhs.015, an Antagonistic Endophytic Actinomycete Isolated from Cucumber Plant

Cited by 11 publications

References 26 publications

Endophytes of Brazilian Medicinal Plants With Activity Against Phytopathogens

Endophytes of Brazilian Medicinal Plants With Activity Against Phytopathogens

Actinomycete Potential as Biocontrol Agent of Phytopathogenic Fungi: Mechanisms, Source, and Applications

A novel protein elicitor (PeSy1) from Saccharothrix yanglingensis induces plant resistance and interacts with a receptor‐like cytoplasmic kinase in Nicotiana benthamiana

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