Investigation on the Binding Mode of 3, 4-Dihydropyrano[c]Chromene Derivative with Double Strand DNA

Dehkordi, Mahvash Farajzadeh; Dehghan, Gholamreza; Mahdavi, Majid; Feizi, Mohammad Ali Hosseinpour

doi:10.15171/apb.2015.065

Cited by 7 publications

(3 citation statements)

References 21 publications

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“…A hypochromic phenomenon refers to the fact that the electronic states of the embedded chromophore and DNA have a strong interaction, causing a decrease in absorbance. 63 The photostability of a photosensitizer is another important factor that should be considered for the PDT application.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…In contrast, no decrease in absorbance of R-CDs was observed in the presence of same concentration of DNA, indicating that R-CDs have no infection to DNA (Figure S7B). A hypochromic phenomenon refers to the fact that the electronic states of the embedded chromophore and DNA have a strong interaction, causing a decrease in absorbance . The photostability of a photosensitizer is another important factor that should be considered for the PDT application.…”

Section: Results and Discussionmentioning

confidence: 99%

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Phosphate-Assisted Transformation of Methylene Blue to Red-Emissive Carbon Dots with Enhanced Singlet Oxygen Generation for Photodynamic Therapy

Wang

Ran

et al. 2021

ACS Appl. Nano Mater.

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Photodynamic therapy (PDT) has attracted wide attention due to its distinct advantages in cancer treatment. Herein, a kind of red emissive carbon dots (R-CDs) was synthesized from methylene blue (MB) and phosphate through a hydrothermal method. The resultant R-CDs display good biocompatibility, photostability, and high singlet oxygen ( 1 O 2 ) yield (0.91); thus, they have been successfully applied to the PDT study in vitro. More importantly, the R-CDs show noninfective property to DNA, which is substantially different to their precursor MB. The structure of R-CDs was comprehensively characterized both experimentally as well as by density functional theory (DFT) calculations. This study not only provides a rational strategy for preparation of highly efficient PDT material but also gives insight into the mechanism of 1 O 2 generation.

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Section: ■ Results and Discussionmentioning

confidence: 99%

Section: Results and Discussionmentioning

confidence: 99%

Phosphate-Assisted Transformation of Methylene Blue to Red-Emissive Carbon Dots with Enhanced Singlet Oxygen Generation for Photodynamic Therapy

Wang

Ran

et al. 2021

ACS Appl. Nano Mater.

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“…UV-vis spectrophotometry, as an impressive and simple technique, is used to monitoring the conformational changes of macromolecules (such as proteins) that occur upon interaction with various ligands. 27 Most of the proteins such as BLC show main absorption bands around 280 nm, which are caused by the existence of aromatic amino acids including tryptophan (Trp), phenylalanine (Phe) and tyrosine (Tyr). 28 Also, BLC has an important absorption peak around 405 nm (Soret-band) due to π→π * transition of electrons in the porphyrin ring of heme group.…”

Section: Resultsmentioning

confidence: 99%

Effects of Resveratrol on the Structure and Catalytic Function of Bovine Liver catalase (BLC): Spectroscopic and Theoretical Studies

et al. 2017

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Purpose: The study on the interaction between various compounds and macromolecules such as enzymes has been very important for monitoring the alteration of structural and functional properties of them. Resveratrol (3, 5, 4-trihydroxy-stilbene; RES) is a biologically active phytoallexin found in grapes and other food products. This article shows an interaction of native bovine liver catalase (BLC) with natural antioxidant product, trans resveratrol (tRES) using multispectroscopic methods.Methods: The interaction between BLC and tRES is performed using UV-vis absorption, fluorescence and circular dichroism (CD) spectroscopy and molecular docking study.Results: In vitro kinetic studies indicated that tRES can decrease BLC activity through uncompetitive inhibition. The results of spectroscopic methods represented that the binding of tRES with BLC can change the micro-region around aromatic amino acids (tryptophan (Trp) and tyrosine (Tyr)) and quench intrinsic fluorescence of BLC by a static mechanism. According to fluorescence quenching data analysis, it was revealed that tRES has one binding site on BLC. The thermodynamic parameters were obtained, which demonstrated that tRES can bind to BLC by van der Waals forces and hydrogen bonds. Molecular docking results indicated that tRES binds to BLC away from heme group and near to the Tyr 324 and Phe 265. These results are in agreement with the experimental results.Conclusion: The inhibitory effect of tRES on BLC demonstrated that excessive consumption of the antioxidants could be resulted in hazardous effects.

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Phenylpyran-fused coumarin novel derivatives: combined photophysical and theoretical study on structural modification for PET-inhibited ICT emission

et al. 2017

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Investigation on the Binding Mode of 3, 4-Dihydropyrano[c]Chromene Derivative with Double Strand DNA

Cited by 7 publications

References 21 publications

Phosphate-Assisted Transformation of Methylene Blue to Red-Emissive Carbon Dots with Enhanced Singlet Oxygen Generation for Photodynamic Therapy

Phosphate-Assisted Transformation of Methylene Blue to Red-Emissive Carbon Dots with Enhanced Singlet Oxygen Generation for Photodynamic Therapy

Effects of Resveratrol on the Structure and Catalytic Function of Bovine Liver catalase (BLC): Spectroscopic and Theoretical Studies

Phenylpyran-fused coumarin novel derivatives: combined photophysical and theoretical study on structural modification for PET-inhibited ICT emission

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