Investigation on the biomimetic influence of biopolymers on calcium phosphate precipitation—Part 1: Alginate

Lima, Daniel Oliveira de; Aimoli, Cassiano Gomes; Beppu, Marisa Masumi

doi:10.1016/j.msec.2008.09.019

Cited by 9 publications

(7 citation statements)

References 21 publications

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“…2). When precipitation is performed at a higher pH, as reported in [7,14], the difference of electrical charges in alginate is greater. As a consequence, a more compact complex without compositional water is obtained.…”

Section: Resultsmentioning

confidence: 86%

“…In this respect, the alginate-based materials are of particular interest. Alginate (extracellular polysaccharide) is a popular biomaterial because of a number of key advantages: convenient precursors, nontoxic, excellent biocompatibility and appropriate biodegradability [5–7]. Additionally, CPs are widely used for bone graft substitution due to their chemical affinity to the bone mineral content [8].…”

Section: Introductionmentioning

confidence: 99%

“…It is well known that alginate allows precipitation of inorganic phases within its macromolecular network [7] and this process provides inorganic phases with different crystals sizes and morphologies [7,12]. For all these reasons, 3D printing of bioinspired structures has gained considerable interest since its inception, primarily because it can lead to artificial bone grafts that are close to native bone.…”

Section: Introductionmentioning

confidence: 99%

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3D printing of mineral–polymer bone substitutes based on sodium alginate and calcium phosphate

Egorov¹,

Fedotov²,

Миронов³

et al. 2016

Beilstein J. Nanotechnol.

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We demonstrate a relatively simple route for three-dimensional (3D) printing of complex-shaped biocompatible structures based on sodium alginate and calcium phosphate (CP) for bone tissue engineering. The fabrication of 3D composite structures was performed through the synthesis of inorganic particles within a biopolymer macromolecular network during 3D printing process. The formation of a new CP phase was studied through X-ray diffraction, Fourier transform infrared spectroscopy and scanning electron microscopy. Both the phase composition and the diameter of the CP particles depend on the concentration of a liquid component (i.e., the “ink”). The 3D printed structures were fabricated and found to have large interconnected porous systems (mean diameter ≈800 μm) and were found to possess compressive strengths from 0.45 to 1.0 MPa. This new approach can be effectively applied for fabrication of biocompatible scaffolds for bone tissue engineering constructions.

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Section: Resultsmentioning

confidence: 86%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

3D printing of mineral–polymer bone substitutes based on sodium alginate and calcium phosphate

Egorov¹,

Fedotov²,

Миронов³

et al. 2016

Beilstein J. Nanotechnol.

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show abstract

“…Alginate is a naturally derived polymer, which has been wildly employed in dispensing-based biofabrication for various applications such as nerve tissue engineering [5,6] and bone tissue engineering [7,8], because of its good biocompatibility and ease gelation in the presence of calcium ions [5,9,10]. And calcium ions have been used to crosslink alginate in order to form hydrogel in dispensing-based biofabrication process.…”

Section: Introductionmentioning

confidence: 99%

Influence of Calcium Ions on Cell Survival and Proliferation in the Context of an Alginate Hydrogel

Cao

Chen

Schreyer

2012

ISRN Chemical Engineering

113

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One goal of biofabrication is to incorporate living cells into artificial scaffolds in order to repair damaged tissues or organs. Although there are many studies on various biofabrication techniques, the maintenance of cell viability during the biofabrication process and cell proliferation after the process is still a challenging issue. Construction of scaffolds using hydrogels composed of natural materials can avoid exposure of cells to harsh chemicals or temperature extremes but can still entail exposure to nonphysiological conditions, causing cell damage or even death. This paper presents an experimental investigation into the influence on Schwann cell survival and proliferation of calcium used for ionic crosslinking of alginate hydrogel during the biofabrication process. The experimental results obtained show the viability and proliferation capacity of cells, either suspended in cell culture medium or encapsulated in hydrogel, and vary with the calcium concentration and the time period of cells exposed to the calcium environment. The experimental results also show the alginate concentration and cell density, that have profound influence on cell survival and proliferation, and solution viscosity as well. This study suggests the incorporation of living cells in calcium-crosslinked hydrogel in the biofabrication process can be regulated for controlled cell survival and proliferation.

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“…3 Polysaccharides are recognized as a strongly influential group of polymers acting upon calcium phosphate and calcium carbonate mineralization that are important classes of minerals in vertebrate and marine hard tissues, respectively. [5][6][7][8][9] Alginate is a linear polysaccharide copolymer, derived from brown sea algae and composed of 1-4 linked β-D-mannuronic acid (M) and α-Lguluronic acid (G) residues with an alternating or block structure. The M-and G-units of alginate differ in the orientation of carboxyl groups that in turn highly affects their functionalty.…”

Section: Introductionmentioning

confidence: 99%

Nucleation and Growth of Brushite in the Presence of Alginate

Ucar

Bjørnøy

Bassett

et al. 2015

Crystal Growth & Design

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Biomineral formation in vivo is a complex process regulated by functional molecules.Highlighting the mechanisms underlying biomineralization is necessary for a better understanding of in vivo processes and for enhanced in vitro model systems. Here, the effect of alginate and its well-defined oligomers with M-or G-block structure on brushite nucleation and growth is investigated by seeded and unseeded experiments. Growth kinetics were studied by seeded experiments and it was shown that molecular weight and functionality of alginate additives affect the crystal growth rates and the growth mechanisms. Growth retardation was most prominent when G-block additives were present. Growth proceeded by surface nucleation when alginate and G-block oligomers were added in the crystallization medium whereas in the presence of M-block oligomers parabolic rate laws were obtained. By decoupling the seeded and experiments, information was deduced on the effects of additives on brushite nucleation. In the presence of alginate and G-block oligomers nucleation was inhibited, however, M-blocks did not show a similar effect. Possible modes of interactions between the mineral and polymer additives are discussed by the evaluation of final crystal morphologies.

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Investigation on the biomimetic influence of biopolymers on calcium phosphate precipitation—Part 1: Alginate

Cited by 9 publications

References 21 publications

3D printing of mineral–polymer bone substitutes based on sodium alginate and calcium phosphate

3D printing of mineral–polymer bone substitutes based on sodium alginate and calcium phosphate

Influence of Calcium Ions on Cell Survival and Proliferation in the Context of an Alginate Hydrogel

Nucleation and Growth of Brushite in the Presence of Alginate

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