Investigation on the effect of vitamin C on growth &amp; biofilm-forming potential of Streptococcus mutans isolated from patients with dental caries

Eydou, Zehdi; Jad, Bader Naser; Elsayed, Zeyad; Ismail, Anas; Magaogao, Michael; Hossain, Ashfaque

doi:10.1186/s12866-020-01914-4

Cited by 19 publications

(16 citation statements)

References 36 publications

(44 reference statements)

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“…In our investigation, the MIC and MBC of ascorbic acid were 12.5 and 25 mg/ml respectively, against S. mutans, whereas in an earlier study, the average MIC and MBC were 9.38 and 10.16 mg/ml, respectively 34 . These MIC and MBC result variations may be due to the susceptibility pattern of the S. mutans strains used, the purity of ascorbic acid, and the experimental settings carried out in different laboratories.…”

Section: Discussioncontrasting

confidence: 77%

Ascorbic Acid from <i>Citrus limon</i> (L.) Osbeck Fruit Source: A Promising Natural Agent for Caries Prevention and Management

Abed

Imran²,

2022

JNR

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Dental caries is an age-old oral disease afflicting humans irrespective of gender, age, socio-economical background, and geographical location. Despite extensive research being done worldwide to prevent dental caries, it is undoubtedly still a significant public health issue. Streptococcus mutans, a commensal bacteria of the oral microflora, is the primary etiological agent strongly linked to dental caries. In recent years, medicinal plant-based phytotherapy has drawn great attention towards managing various diseases as it is effective and safe. The aim of the investigation was to evaluate the antibacterial activity of fresh Citrus limon fruit juice against S. mutans and to quantify its ascorbic acid (Vitamin C) concentration. Additionally, to determine the MIC, MBC and growth curve assay of standard ascorbic acid against S. mutans. Fresh C. limon fruit juice demonstrated significant antimicrobial activity against the S. mutans reference strain (MTCC 497) by the agar well diffusion method. The ascorbic acid concentration of C. limon juice was found to be 0.194 % w/w by HPLC technique. The MIC and MBC values of standard ascorbic acid against S. mutans were found to be 12.5 mg/ml and 25 mg/ml, respectively. The growth curve assay demonstrated that ascorbic acid at a sub-MIC concentration of 6.25 mg/ml did not display any significant variations in the growth rate of S. mutans. To summarize, C. limon fruit juice is a potential source of natural antibacterial metabolites against S. mutans. Ascorbic acid, one of the chief components of C. limon fruit, is a promising agent for the development of anti-caries products. Further studies are warranted for developing novel formulations using ascorbic acid with other natural metabolites for the prevention and better management of caries.

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Section: Discussioncontrasting

confidence: 77%

Ascorbic Acid from <i>Citrus limon</i> (L.) Osbeck Fruit Source: A Promising Natural Agent for Caries Prevention and Management

Abed

Imran²,

2022

JNR

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“…There was up to a 92% reduction in biofilm production. Likewise, Sendamangalam et al (2011) and Eydou et al (2020) reported 2 mg/ml and 5.61 mg/ml, respectively, as the biofilm inhibitory concentration of vitamin C against S. mutans.…”

Section: Discussionmentioning

confidence: 96%

Assessment of Antibacterial and Anti-biofilm Effects of Vitamin C Against Pseudomonas aeruginosa Clinical Isolates

et al. 2022

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There is a persistent need to look for alternative therapeutic modalities to help control the pandemic of antimicrobial resistance. Assessment of antibacterial and anti-biofilm effects of vitamin C (ascorbic acid) was the aim of the current study. The micro-dilution method determined the minimal inhibitory concentration (MIC) of ascorbic acid or antibiotics alone and in combinations against Pseudomonas aeruginosa (P. aeruginosa) clinical isolates. The micro-titer plate method monitored the effect of ascorbic acid on the biofilm-producing isolates of P. aeruginosa. The effect of ascorbic acid on the differential expression of different antibiotic-resistant genes and biofilm encoding genes of P. aeruginosa isolates were also tested using real-time polymerase chain reaction (PCR). For in vivo assessment of the antibacterial effects of ascorbic acid alone or combined with an antibiotic, rats were infected with P. aeruginosa clinical isolate followed by different treatment regimens. MICs of ascorbic acid among P. aeruginosa isolates were in the range of 156.2–1,250 μg/ml, while MIC50 and MIC90 were 312.5 and 625 μg/ml, respectively. At sub-inhibitory concentrations (19.5–312.5 μg/ml), ascorbic acid had 100% biofilm inhibitory effect. Furthermore, ascorbic acid-treated bacteria showed downregulation of genes underpinning biofilm formation and antibiotic resistance. In vivo assessment of vitamin C and ceftazidime in rats showed that administration of both at a lower dose for treatment of pseudomonas infection in rats had a synergistic and more powerful effect. Vitamin C shows excellent in vitro results as an antibacterial and anti-biofilm agent. Vitamin C should be routinely prescribed with antibiotics to treat bacterial infections in the clinical setting.

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“…Ascorbic acid, commonly known as Vitamin C, is an essential dietary nutrient that is necessary for a variety of physiological processes in the human body, including wound repair and collagen production [ 27 ]. Ascorbic acid is also an antioxidant [ 28 ] with antibacterial activity [ 29 ], and the ability to disrupt exopolysaccharide biofilm formation in E. coli [ 30 ], S. mutans [ 31 ], B. subtilis [ 32 ], and S. aureus [ 33 ]. Ascorbic acid is a glycation inhibitor [ 25 , 34 ] via its antioxidant activity and ability to ionically bind to proteins, competitively inhibiting the binding of glucose.…”

Section: Discussionmentioning

confidence: 99%

Staphylococcus aureus Biofilm Inhibiting Activity of Advanced Glycation Endproduct Crosslink Breaking and Glycation Inhibiting Compounds

2022

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Staphylococcus aureus is a Gram-positive bacterium that plays a role in the pathogenesis of skin lesions in diabetes mellitus, atopic dermatitis, and psoriasis, all of which are associated with elevated non-enzymatic glycation biomarkers. The production of biofilm protects resident bacteria from host immune defenses and antibiotic interventions, prolonging pathogen survival, and risking recurrence after treatment. Glycated proteins formed from keratin and glucose induce biofilm formation in S. aureus, promoting dysbiosis and increasing pathogenicity. In this study, several glycation-inhibiting and advanced glycation endproduct (AGE) crosslink-breaking compounds were assayed for their ability to inhibit glycated keratin-induced biofilm formation as preliminary screening for clinical testing candidates. Ascorbic acid, astaxanthin, clove extract, n-phenacylthiazolium bromide, and rosemary extract were examined in an in vitro static biofilm model with S. aureus strain ATCC 12600. Near complete biofilm inhibition was achieved with astaxanthin (ED50 = 0.060 mg/mL), clove extract (ED50 = 0.0087 mg/mL), n-phenacylthiazolium bromide (ED50 = 5.3 mg/mL), and rosemary extract (ED50 = 1.5 mg/mL). The dosage necessary for biofilm inhibition was not significantly correlated with growth inhibition (R2 = 0.055. p = 0.49). Anti-glycation and AGE breaking compounds with biofilm inhibitory activity are ideal candidates for treatment of S. aureus dysbiosis and skin infection that is associated with elevated skin glycation.

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Investigation on the effect of vitamin C on growth & biofilm-forming potential of Streptococcus mutans isolated from patients with dental caries

Cited by 19 publications

References 36 publications

Ascorbic Acid from <i>Citrus limon</i> (L.) Osbeck Fruit Source: A Promising Natural Agent for Caries Prevention and Management

Ascorbic Acid from <i>Citrus limon</i> (L.) Osbeck Fruit Source: A Promising Natural Agent for Caries Prevention and Management

Assessment of Antibacterial and Anti-biofilm Effects of Vitamin C Against Pseudomonas aeruginosa Clinical Isolates

Staphylococcus aureus Biofilm Inhibiting Activity of Advanced Glycation Endproduct Crosslink Breaking and Glycation Inhibiting Compounds

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