Investigation on the In Vitro Degradation and Release Behaviors of Calcium Phosphate Containing Chinese Medicine

Abstract

The purpose of this study was to investigate the in vitro degradation and release behaviors of calcium phosphate powders (Ca/P) containing Chinese Medicine for bone graft. Two kinds of Ca/P powders, one with the Chinese Medicine, Salvia Miltiorrhiza Bunge (SMB) and the other with Polyacrylic Acid (PAA) and SMB, were soaked in simulated body fluid (SBF) for up to 150h. The in vitro release of SMB was measured by UV-VIS spectroscopy. The Ca2+ concentrations and pH of SBF soaked Ca/P powders were measured by AAS and pH meter. TA and XRD were employed to analyze various Ca/P powders before and after soaked in SBF, respectively. The results demonstrated that a faster SMB release occurred during the first 24 hours, while a slow release was sustained up to150h. Furthermore, the released of SMB in Ca/P-PAA-SMB was faster than that of Ca/P-SMB. It was concluded that a controllable release of Chinese Medicine from Calcium phosphate may be carried out by the addition of suitable surfactant. Accordingly, calcium ions were released into SBF, which was benefit for bone tissue repairing and reconstructing. Additionally, the TA results showed that there was 1.66% SMB released from Ca/P-SMB-PAA powders. XRD confirmed that various Ca/P powders possessed poorer crystallinity and smaller grain size.