Investigation on the interaction between myricetin and dihydromyricetin with trypsin, α‐chymotrypsin, lysozyme by spectroscopy and molecular docking methods

Meng, Xianxin; Nan, Guanjun; Shi, Bowen; Li, Wanlu; Liu, Henglin; Lin, Rong; Yang, Guangde

doi:10.1002/bio.4225

Cited by 8 publications

(2 citation statements)

References 43 publications

(41 reference statements)

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“…Studies indicate that naringin can diminish the binding stability of lovastatin with pepsin and α ‐chymotrypsin, thereby elevating the concentration of free lovastatin (Yang et al, 2020). Nitrendipine and nimodipine have been shown in studies to be significantly affected by vitamin C in terms of its binding constants with human serum albumin and lysozyme (Meng et al, 2022, 2023). However, there are no reports on the influence of vitamin C on the pharmacokinetic effect.…”

Section: Introductionmentioning

confidence: 99%

Effects of vitamin C on the pharmacokinetics and pharmacodynamics of nimodipine in rats

Meng,

Nan,

et al. 2024

Biomedical Chromatography

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In recent years, researchers have shown a growing interest in the interactions between different pharmaceutical agents. An intriguing instance lies in the possible interaction between nimodipine and vitamin C. To investigate the pharmacokinetic and pharmacodynamic effects of vitamin C on nimodipine in rats, rats were randomly divided into a nimodipine only group and a combination group (nimodipine + vitamin C). The two groups were given intragastric administration and nimodipine blood concentrations were determined using high‐performance liquid chromatography–tandem mass spectrum at different time points. Blood pressure and heart rate were measured via carotid artery cannulation. Pharmacokinetic differences were observed between the nimodipine only group and the combination group at the same dose. Compared with the nimodipine only group, the combination group’s main pharmacokinetic parameters of peak concentration and area under the curve increased significantly, and the difference was statistically significant (p < 0.05); furthermore, the combination group exhibited a significant reduction in average blood pressure, while no significant effects on heart rate were observed. Vitamin C did not affect the activity of CYP450 in rat liver. The pharmacokinetic characteristics and pharmacodynamics of nimodipine were changed by vitamin C administration in rats.

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Section: Introductionmentioning

confidence: 99%

Effects of vitamin C on the pharmacokinetics and pharmacodynamics of nimodipine in rats

Meng,

Nan,

et al. 2024

Biomedical Chromatography

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“…Pepsin, trypsin and α-chymotrypsin are all present in the digestive system, which can degrade or hydrolyze proteins. [10][11][12] Pepsin is mainly released by cells in the stomach to break down food into peptides. [13] It is an endopeptidase that can catalyze the hydrolytic cleavage of digestive bonds near hydrophobic or aromatic amino acid residues.…”

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confidence: 99%

Interaction between caffeic acid phenethyl ester and protease: monitoring by spectroscopic and molecular docking approaches

et al. 2022

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The interaction of one anticancer drug (caffeic acid phenethyl ester; CAPE) with three proteases (trypsin, pepsin and α-chymotrypsin) has been investigated with multispectral methods and molecular docking. As an active components in propolis, the findings are of great benefit to metabolism, design, and structural modification of drugs. The results show that CAPE has an obvious ability to quench the trypsin, pepsin, or α-chymotrypsin fluorescence mainly through a static quenching procedure.Trypsin has the largest binding affinity to CAPE, and α-chymotrypsin has the smallest binding affinity to CAPE. The data obtained from thermodynamic parameters and molecular docking prove that the spontaneously interaction between CAPE and each protease is mainly due to a combination of van der Waals (vdW) force and hydrogen bond (H-bond), controlled by an enthalpy-driven process. The binding force, strength, position, and the number of H-bond are further obtained from the results of molecular docking. Through ultraviolet spectroscopy, dynamic light scattering and circular dichroism experiments, the change in the protease secondary structure induced by CAPE was observed. Additionally, the addition of protease had a positive effect on the antioxidative activity of CAPE, and α-chymotrypsin has the greatest effect on the removal of 2,2-diphenyl-1-picrylhydrazyl free radicals by CAPE.α-chymotrypsin, caffeic acid phenethyl ester (CAPE), pepsin, trypsin | INTRODUCTIONCaffeic acid phenethyl ester (CAPE) has been widely used in various regions as a traditional natural medicine. [1] As the main active ingredient of propolis, CAPE has various biological activities, including anticancer, anti-inflammatory, antibacterial, neuroprotective, etc. [2][3][4] In addition, it has a very strong antioxidant capacity, [5] as a polyphenol containing hydroxyl groups in benzenediol. [6] In previous works, the study of the interaction between CAPE and macromolecules mainly focus on the binding mechanism, influencing factors, drug activities, and pharmacology; many theoretical researches have been done.Studies have shown that poly(3-hydroxybutyrate) (PHB) fibre can enhance the antioxidant and antibacterial activities of CAPE. [7] CAPE can inhibit iNOS gene transcription by inhibiting the nuclear factor NF-κB site, and can inhibit the catalytic activity of iNOS, [8] which provides a research basis for the anti-inflammatory and antitumour effects of CAPE. According to reports, after CAPE is compounded with γ-cyclodextrin (γ-CD), the anticancer activity of CAPE will increase. [9] In addition, in the work of this research group, the binding constant of CAPE and bovine serum albumin was $10 6 , of which the main forces are H-bond and vdW force, and the driving mode is enthalpy drive. However, there are few studies on the interaction

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A comparison of different cleaning approaches for blood contamination after curing universal adhesives on the dentine surface

Liu,

Xie,

Chen

2024

Dental Materials

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Investigation on the interaction between myricetin and dihydromyricetin with trypsin, α‐chymotrypsin, lysozyme by spectroscopy and molecular docking methods

Cited by 8 publications

References 43 publications

Effects of vitamin C on the pharmacokinetics and pharmacodynamics of nimodipine in rats

Effects of vitamin C on the pharmacokinetics and pharmacodynamics of nimodipine in rats

Interaction between caffeic acid phenethyl ester and protease: monitoring by spectroscopic and molecular docking approaches

A comparison of different cleaning approaches for blood contamination after curing universal adhesives on the dentine surface

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