Investigation on the interaction of collagen molecules in solution with different acetic acid concentrations

Yang, Huan; Deng, Yi; Xu, Songcheng; Liu, Wentao; Li, Guoying

doi:10.1002/app.45255

Cited by 11 publications

(10 citation statements)

References 47 publications

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“…[3] Moreover, the addition of CNT endows polymer composites with excellent electromagnetic interference shielding properties. [4][5][6] Recently, there has been increasing research attention in microinjection molding (μIM) of filler-containing polymer composites, [7][8][9][10][11] thanks to their multifunctional performance, especially for CNT-filled polymer composites.…”

Section: Introductionmentioning

confidence: 99%

Electrical, thermal, and mechanical properties of polypropylene/multiwalled carbon nanotube micromoldings

2019

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In this paper, properties of microparts molded from polypropylene/multiwalled carbon nanotubes (PP/CNT) composites were studied in detail. Results indicated that the percolation threshold of PP/CNT microparts shifted to higher filler concentrations when compared with their compression molded counterparts. Morphology observations revealed that the distribution of CNT is not uniform along the flow direction, which can significantly affect the properties of microparts. The thermal properties of PP and PP/CNT composites as well as corresponding microparts were investigated using differential scanning calorimetry and thermogravimetric analysis, respectively. Moreover, the mechanical properties of microparts were evaluated by nanoindentation analysis. Multicycling measurements indicated that the mechanical properties of microparts are both depth and location dependent. Both the hardness and elastic modulus increased with corresponding filler loading concentration of CNT below 3 and 7 wt%, respectively. Above those concentrations, both properties decreased with an increase of CNT concentration. This could be related to the state of filler distribution within the microparts.

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Section: Introductionmentioning

confidence: 99%

Electrical, thermal, and mechanical properties of polypropylene/multiwalled carbon nanotube micromoldings

2019

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“…Thermodynamic rigidity of collagen and its affinity to the solvent can be obtained from Huggins co‐efficient, K H where a range of 0.5–0.8 denotes that the polymer is a flexible chain. Values below and above the scale indicate that the collagen is semi‐rigid and stiff triple helix chains 29 . Thus from the values obtained, the triple helical chain of scale collagen of Piranha and Rohu are semi‐rigid and stiff respectively.…”

Section: Resultsmentioning

confidence: 80%

“…Values below and above the scale indicate that the collagen is semi-rigid and stiff triple helix chains. 29 Thus from the values obtained, the triple helical chain of scale collagen of Piranha and Rohu are semi-rigid and stiff respectively.…”

Section: Intrinsic Viscositymentioning

confidence: 90%

“…The efflux time of the solution from the viscometer was recorded and the intrinsic viscosity was determined from the intercept of the plot η sp /C versus C and using the Huggins equation:

η_{sp} / normalC = ([], η) + K_{H} {[η]}^{2} normalC

Where η sp , specific viscosity is ([ t – t o ]/ t o ), t and t o , the time of collagen solutions and solvent passing through capillary respectively at the same temperature, K H , the Huggins coefficient and C, concentration of the collagen solution (g/dl). The values reveal the molecular interaction between the collagen molecules in the solution 29 …”

Section: Methodsmentioning

confidence: 99%

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Thermally stable collagen from Piranha and Rohu with improved physical, biochemical, and morphological properties

Paul

Padmapriya

2021

J of Applied Polymer Sci

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Collagen is the most common structural protein pertinent to the skin and other tissues in humans. The market for collagen extends from food, cosmetics to therapeutics and is estimated to be $6.63 billion by 2025. Piranha and Rohu are some of the primarily consumed fish varieties in South India and the extraction of collagen from the scales of Piranha is reported for the first time through this study. Physical, biochemical, and morphological properties of the collagen extracted were compared with a freshwater variant, Rohu. The UV, IR spectra, amino acid, SDS PAGE analysis confirmed the presence of triple helical native conformation. Further, higher degree of solubility was observed at low pH and salt concentrations. The Td was 38.27 and 37.4°C and Tm was 92.37 and 94.04°C for the scale collagen of Piranha and Rohu respectively and was comparable to bovine sources. Electron micrograph presented loose, parallel oriented long fibers with interconnected fibrils. Hence the collagen extracted, having retained the helical conformation and higher thermal stability, can be a safe alternative for various biomedical applications.

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“…For this reason, all tested proteins were dissolved in 500 mM acetic acid in this study. The critical aggregation concentration (CAC) of calf skin collagen has been reported to be ∼500 μg/mL in 100 mM acetic acid [41]. Therefore, concentrations greater than this were avoided.…”

Section: Resultsmentioning

confidence: 99%

Quantitation of native and forced degraded collagens by capillary zone electrophoresis: Method development and validation

Olabi,

Wätzig

2023

J of Separation Science

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A new capillary zone electrophoresis method for collagen quantitation was developed and validated according to the International Council for Harmonization guideline Q2 (R1). The Sircol collagen assay and ultraviolet spectrometry were employed as reference methods. Capillary zone electrophoresis enables specific, simple, and fast determination within 9 min. It is less user‐dependent and more automated than the Sircol collagen assay. With a limit of detection of 18.0 μg/mL, the new method is less sensitive than the Sircol collagen assay, which has a limit of detection of 6.5 μg/mL. Nonetheless, capillary zone electrophoresis covers a wider linearity range (50‐400 μg/mL) compared to the Sircol collagen assay (5‐80 μg/mL), with similar precision. Additional advantages of capillary zone electrophoresis are the ability to gain information on collagen integrity and to simultaneously determine native and denatured collagens. This approach represents a modern and legitimate alternative to the Sircol collagen assay. The developed method has been successfully applied to the study of three collagen products and samples from forced degradation.

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Investigation on the interaction of collagen molecules in solution with different acetic acid concentrations

Cited by 11 publications

References 47 publications

Electrical, thermal, and mechanical properties of polypropylene/multiwalled carbon nanotube micromoldings

Electrical, thermal, and mechanical properties of polypropylene/multiwalled carbon nanotube micromoldings

Thermally stable collagen from Piranha and Rohu with improved physical, biochemical, and morphological properties

Quantitation of native and forced degraded collagens by capillary zone electrophoresis: Method development and validation

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