Investigation on the transcription factors of porcine 3β-hydroxysteroid dehydrogenase and 17β-hydroxysteroid dehydrogenase genes

Dong, Xinxing; Bai, Ying; Yi, Xin; Xu, Qinqin; Chen, Gang; Fang, Meiying

doi:10.1016/j.gene.2012.02.039

Cited by 4 publications

(3 citation statements)

References 17 publications

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“…However, there is very limited information regarding regulation of 17βHSD7 gene expression in pig liver. In our recent study using luciferase activity assay and EMSA, we found a steroid-related TF (TALE) in the porcine liver anchoring −850 to −868 bp of promoter region of 17βHSD7 gene [18]. A is generally known as a pheromone and does not have any hormonal function in mammals [19].…”

Section: Discussionmentioning

confidence: 99%

Investigation of Testosterone, Androstenone, and Estradiol Metabolism in HepG2 Cells and Primary Culture Pig Hepatocytes and Their Effects on 17βHSD7 Gene Expression

Chen

Dong

et al. 2012

PLoS ONE

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Steroid metabolism is important in various species. The accumulation of androgen metabolite, androstenone, in pig adipose tissue is negatively associated with pork flavor, odour and makes the meat unfit for human consumption. The 17β-hydroxysteroid dehydrogenase type 7 (17βHSD7) expressed abundantly in porcine liver, and it was previously suggested to be associated with androstenone levels. Understanding the enzymes and metabolic pathways responsible for androstenone as well as other steroids metabolism is important for improving the meat quality. At the same time, metabolism of steroids is known to be species- and tissue-specific. Therefore it is important to investigate between-species variations in the hepatic steroid metabolism and to elucidate the role of 17βHSD7 in this process. Here we used an effective methodological approach, liquid chromatography coupled with mass spectrometry, to investigate species-specific metabolism of androstenone, testosterone and beta-estradiol in HepG2 cell line, and pig cultured hepatocytes. Species- and concentration-depended effect of steroids on 17βHSD7 gene expression was also investigated. It was demonstrated that the investigated steroids can regulate the 17βHSD7 gene expression in HepG2 and primary cultured porcine hepatocytes in a concentration-dependent and species-dependent pattern. Investigation of steroid metabolites demonstrated that androstenone formed a 3′-hydroxy compound 3β-hydroxy-5α-androst-16-ene. Testosterone was metabolized to 4-androstene-3,17-dione. Estrone was found as the metabolite for β-estradiol. Inhibition study with 17βHSD inhibitor apigenin showed that apigenin didn’t affect androstenone metabolism. Apigenin at high concentration (50 µM) tends to inhibit testosterone metabolism but this inhibition effect was negligible. Beta-estradiol metabolism was notably inhibited with apigenin at high concentration. The study also established that the level of testosterone and β-estradiol metabolites was markedly increased after co-incubation with high concentration of apigenin. This study established that 17βHSD7 is not the key enzyme responsible for androstenone and testosterone metabolism in porcine liver cells.

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Section: Discussionmentioning

confidence: 99%

Investigation of Testosterone, Androstenone, and Estradiol Metabolism in HepG2 Cells and Primary Culture Pig Hepatocytes and Their Effects on 17βHSD7 Gene Expression

Chen

Dong

et al. 2012

PLoS ONE

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“…However, the molecular regulation of porcine 3b-HSD gene transcription is not fully elucidated. It has been reported that a transcription factor belonging to the proline and acidic amino acid-rich basic leucine zipper (PAR/bZIP) family is involved in the porcine hepatic 3b-HSD gene regulation (Dong et al 2012). In our previous work, we have found that CCAAT/ enhancer binding protein b (C/EBPb) (Li et al 2013) and glucocorticoid receptor (GR) (Li et al 2015) are respectively responsible for the breed-dependent expression of 3b-HSD in porcine adrenal gland and liver.…”

Section: Introductionmentioning

confidence: 99%

“…It has been reported that a transcription factor belonging to the proline and acidic amino acid‐rich basic leucine zipper (PAR/bZIP) family is involved in the porcine hepatic 3β‐HSD gene regulation (Dong et al . ). In our previous work, we have found that CCAAT/enhancer binding protein β (C/EBPβ) (Li et al .…”

Section: Introductionmentioning

confidence: 99%

Glucocorticoid receptor is involved in the differential expression of hepatic 3β‐hydroxysteroid dehydrogenase between barrows and boars at finishing stage

Cong

Yao

et al. 2017

Animal Science Journal

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The enzyme 3β-hydroxysteroid dehydrogenase (3β-HSD) plays an important role in androstenone metabolism in pig liver, and its defective expression is related to the development of boar taint. Early age castration is a common practice in many countries to avoid boar taint, yet whether and how castration affects porcine hepatic 3β-HSD expression are still poorly understood. In this study, we aimed to compare the expression of 3β-HSD between intact (boars) and castrated (barrows) male pigs, and to explore the potential factors regulating 3β-HSD transcription. Compared to barrows, boars showed worse carcass quality. Boars had significantly higher levels of serum androstenone (P < 0.01), testosterone (P < 0.01) and hepatic cortisol (P < 0.05), which were contrary to significantly lower expression of 3β-HSD messenger RNA (P < 0.01) and protein (P < 0.01) in the liver. Significant differences were detected for the hepatic expression of androgen receptor (AR) and CCAAT/enhancer binding protein β (C/EBPβ). Chromatin immunoprecipitation (ChIP) assay demonstrated reduced histone H3 acetylation (P < 0.05) but increased glucocorticoid receptor (GR) binding to 3β-HSD gene promoter in boars (P < 0.05). These results indicate that GR binding to 3β-HSD promoter is involved in the differential hepatic 3β-HSD expression between boars and barrows.

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Regulation of 3β-hydroxysteroid dehydrogenase and sulphotransferase 2A1 gene expression in primary porcine hepatocytes by selected sex-steroids and plant secondary metabolites from chicory (Cichorium intybus L.) and wormwood (Artemisia sp.)

Rasmussen

Ekstrand

2014

Gene

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Investigation on the transcription factors of porcine 3β-hydroxysteroid dehydrogenase and 17β-hydroxysteroid dehydrogenase genes

Cited by 4 publications

References 17 publications

Investigation of Testosterone, Androstenone, and Estradiol Metabolism in HepG2 Cells and Primary Culture Pig Hepatocytes and Their Effects on 17βHSD7 Gene Expression

Investigation of Testosterone, Androstenone, and Estradiol Metabolism in HepG2 Cells and Primary Culture Pig Hepatocytes and Their Effects on 17βHSD7 Gene Expression

Glucocorticoid receptor is involved in the differential expression of hepatic 3β‐hydroxysteroid dehydrogenase between barrows and boars at finishing stage

Regulation of 3β-hydroxysteroid dehydrogenase and sulphotransferase 2A1 gene expression in primary porcine hepatocytes by selected sex-steroids and plant secondary metabolites from chicory (Cichorium intybus L.) and wormwood (Artemisia sp.)

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