Investigation the influence of biologically active compounds on the antioxidant, antibacterial and anti-inflammatory activities of red raspberry (<i>Rubus idaeous</i> l.) leaf extract

Maslov, O. Yu.; Komisarenko, M. A.; Ponomarenko, Svitlana V.; Horopashna, Darina; Osolodchenko, Tetiana; Kolisnyk, S. V.; Derymedvid, L. V.; Yu, Klimenko Lina; Akhmedov, E. Yu.

doi:10.2478/cipms-2022-0040

Cited by 12 publications

(7 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although there are many different sorts of raspberries, the most prevalent are red and black. Raspberry fruits, leaves, and blooms have been used extensively in traditional medical practices [21]. Raspberry fruits contain vitamin C and some B vitamins (thiamin, riboflavin, pantothenic, and folate), and also include significant levels of manganese which are beneficial to health and may lower the risk of chronic diseases in the modern world [11].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Antioxidant, Anti-inflammatory, and Antimicrobial Activities of Raspberry Fruit Extract: An In Vitro Study

Gomathi,

Umamaheswari,

Prethipa

2024

Cureus

View full text Add to dashboard Cite

Introduction Rubus idaeus (R. idaeus) is an edible fruit that contains numerous significant bioactive compounds that hold important biological properties and are categorized as nutraceuticals owing to the health benefits it imparts including decreasing the risk of chronic diseases like diabetes mellitus, cancer, heart disease, and many other diseases. The objective of the present research was to explore the antimicrobial, antioxidant, and anti-inflammatory characteristics of the aqueous raspberry extract through in vitro assays. Materials and methods R. idaeus aqueous extract was prepared and examined for its antimicrobial activity against Streptococcus mutans (S. mutans) bacteria and Candida albicans (C. albicans) fungi using the agar-well diffusion method, and the antioxidant activity was evaluated using the DPPH (2, 2-Diphenyl-1-picrylhydrazyl hydrate) radical scavenging assay and the hydrogen peroxide radical scavenging assay. The anti-inflammatory activity of the prepared extract was investigated using bovine serum albumin (BSA) and egg albumin denaturation assays. Results R. idaeus fruit extract displayed strong antimicrobial activity at a higher concentration of 100 µL with a 26 mm zone of inhibition against Streptococcus mutans and 24 mm for Candida albicans. The extract showed 87.42% hydrogen peroxide free radical scavenging activity and inhibited 91.12% DPPH free radicals at the highest concentration of 50 µg/mL. The extract showed effective anti-inflammatory activity by preventing the denaturation of bovine serum albumin (80%) and egg albumin proteins (77%) at the highest concentration of 50 μg/mL. The free radical scavenging activity positively correlates with the increased concentration of the prepared extract against DPPH and hydrogen peroxide free radicals, thus showing the raspberry extract's potent antioxidant activity. Similarly, the anti-inflammatory assay result shows that the prepared raspberry aqueous extract has excellent anti-inflammatory activity by preventing the denaturation of bovine serum albumin and egg albumin protein in a dose-dependent manner. Conclusion The meticulously prepared raspberry extract exhibited noteworthy antimicrobial, antioxidant, and anti-inflammatory characteristics, and owing to its astounding therapeutic benefits it holds a tremendous promise as a natural alternative in the field of oral medicine especially in the management of oral mucosal lesions, oral potentially malignant lesions such as lichen planus and leukoplakia, candidiasis, oral cancer and oral mucositis. Further animal studies and clinical trials are recommended to fully reap the therapeutics potential of raspberry extract.

show abstract

Section: Discussionmentioning

confidence: 99%

Evaluation of Antioxidant, Anti-inflammatory, and Antimicrobial Activities of Raspberry Fruit Extract: An In Vitro Study

Gomathi,

Umamaheswari,

Prethipa

2024

Cureus

View full text Add to dashboard Cite

show abstract

“…Leaves are less studied than fruits probably because of the wide use of fruit in human nutrition. However, the antimicrobial and antiviral properties of plants have also begun to be more actively researched after the breakthrough in packaging production in recent years, as evidenced by various research studies on films [ 31 , 32 ]. Kucharskii et al [ 33 ] stated that the raspberry leaves of the genus Rubus can be applied as antiseptic agents in the treatment of skin diseases.…”

Section: Resultsmentioning

confidence: 99%

Correlation between Antimicrobial Activity Values and Total Phenolic Content/Antioxidant Activity in Rubus idaeus L.

Ispiryan,

Atkociuniene,

Makstutiene

et al. 2024

Plants

View full text Add to dashboard Cite

Plant by-products, which are discarded into the environment, are rich in valuable compounds. The aim of this research was to determine the antibacterial activity of Rubus idaeus L. morphological parts and its correlation with total phenolic content and antioxidant activity. The authors also aimed to evaluate the plant’s potential as added-value products. New aspects were revealed for further use and for making novel and natural products. The study’s results indicated that raspberry leaves, inflorescences, and fruits could effectively combat three Gram-positive bacteria. According to the findings, among the various plant parts, root and seed extracts had the lowest antibacterial activity. Data revealed moderate, weak, or very weak correlation between the antimicrobial activity and phenolic content parameters. These findings underscore the viability of substituting synthetic antimicrobials with natural alternatives. The present study is significant for preparing novel products as antibacterials by appropriate and optimized processing using all raspberry morphological parts, and the research results show promising prospects for future purposeful utilisation of nature-based products. Raspberry plant parts can find applications in emerging fields that generate economic and environmental value.

show abstract

“…Antioxidant activity. The antioxidant activity was assessed using the potentiometric method [14,15,16]. Antioxidant activity was calculated according to the following equation and expressed as mmol-equiv./L .…”

Section: Methodsmentioning

confidence: 99%

Investigation of antioxidant interaction between ascorbic acid and rutin in medicine “Ascorutin”

Maslov,

Komisarenko,

Kolisnyk

et al. 2024

Current issues in pharmacy and medicine: science and practice

View full text Add to dashboard Cite

Phytomedicines are combined pharmaceuticals containing various groups of phenolic compounds. It is known that phenolic compounds are powerful antioxidants, but their level of antioxidant activity during interactions has not been previously studied. The aim of the work was to investigate interaction between ascorbic acid and rutin in medicine “Ascorutin”. Materials and methods. The objects of the study were one series of tablets “Ascorutin,” rutin, and ascorbic acid. The level of antioxidant activity was measured by the potentiometric method. Results. The level of antioxidant activity of model solutions of rutin was 265.51 ± 1.00 mmol-eq./L, ascorbic acid – 99.00 ± 1.00 mmol-eq./L, a combination of rutin and ascorbic acid – 255.60 ± 1.00 mmol-eq./L, and medicine “Ascorutin” – 256.52 ± 1.00 mmol-eq./L. An experimental value of the level of antioxidant activity of “Ascorutin”, the combination of rutin and ascorbic acid was 30 % less than theoretical values. Conclusions. The antioxidant interaction of ascorbic acid and rutin in “Ascorutin” medicine has been studied. It has been established that the combination of rutin and ascorbic acid has an antagonistic effect. The findings of this research will serve as a stimulus for exploring the interaction of bioactive substances and selecting their optimal combinations for the development of pharmaceuticals, special food products, food, and cosmetic products.

show abstract

Investigation the influence of biologically active compounds on the antioxidant, antibacterial and anti-inflammatory activities of red raspberry (Rubus idaeous l.) leaf extract

Cited by 12 publications

References 27 publications

Evaluation of Antioxidant, Anti-inflammatory, and Antimicrobial Activities of Raspberry Fruit Extract: An In Vitro Study

Evaluation of Antioxidant, Anti-inflammatory, and Antimicrobial Activities of Raspberry Fruit Extract: An In Vitro Study

Correlation between Antimicrobial Activity Values and Total Phenolic Content/Antioxidant Activity in Rubus idaeus L.

Investigation of antioxidant interaction between ascorbic acid and rutin in medicine “Ascorutin”

Contact Info

Product

Resources

About