Investigations into the natural infection rate of Haemaphysalis qinghaiensis with Piroplasma using a nested PCR

Sun, Caiqin; Liu, Zhijie; Gao, Jinliang; Guan, Guiquan; Ma, Miling; Luo, Jianxun; Yin, Hong

doi:10.1007/s10493-008-9133-2

Cited by 15 publications

(15 citation statements)

References 20 publications

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“…One hundred thirty-six of them were separated as described previously (Sun et al 2008). Each tick was soaked in 70% ethanol in 15 min, dried, and grounded in a separate 1.5 ml tube to avoid cross contamination.…”

Section: Collection Of Haemaphysalis Qinghaiensis Tick Samples and Dnmentioning

confidence: 99%

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Simultaneous detection of piroplasma infections in field Haemaphysalis qinghaiensis ticks by reverse line blotting

et al. 2011

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Piroplasmosis is caused by tick-borne haemoprotozoan parasites in the genera of Theileria and Babesia, in which numbers of agents are highly pathogenic for cattle, sheep and goats. We developed a reverse line blot (RLB) assay for detection and differentiation of four different parasites, in which 18S ribosomal RNA gene sequence was amplified with a set of universal primers specific for all members in the genera of Theileria and Babesia; and the probes were designed on the basis of hypervariable region 4 (V4 region) of 18S rRNA gene. Three Theileria and one Babesia can be detected simultaneously on this system and it was sensitive to detect a parasitemia level between 10(-5) and 10(-8)%. A total of 149 Haemaphysalis qinghaiensis ticks collected from Lintan County of Gannan Tibetan Autonomous Region was tested by RLB. Among these, 136 tick samples were also tested by a nested PCR assay developed previously. After comparison of these results, it showed that more T. luwenshuni was detected in RLB assay, while more T. uilenbergi was detected in H. qinghaiensis ticks by nested PCR. The RLB has shown capability for simultaneous detection of four species of piroplasm in H. qinghaiensis ticks, indicating its usefulness for epidemiological studies of piroplasmosis.

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Section: Collection Of Haemaphysalis Qinghaiensis Tick Samples and Dnmentioning

confidence: 99%

“…Based on the 18S rRNA gene sequencing, PCR (Sun et al 2008) was developed for distinguishing between these four species. However, PCR assays do not generally detect mixed infections, although there are some amplification protocols that can detect mixed piroplasm infections to some extent (Birkenheuer et al 2003;Criado-Fornelio et al 2003).…”

Section: Introductionmentioning

confidence: 99%

Simultaneous detection of piroplasma infections in field Haemaphysalis qinghaiensis ticks by reverse line blotting

et al. 2011

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“…T. sinensis is transmitted by Haemaphysalis qinghaiensis ticks and infects cattle, yak 2004;Sun et al, 2008) and water buffalo (He et al, 2012) in China.…”

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confidence: 99%

Phylogeny of Theileria buffeli genotypes identified in the South African buffalo (Syncerus caffer) population

Chaisi

Collins

Oosthuizen

2014

Veterinary Parasitology

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Theileria buffeli/T. sergenti/T. orientalis is a group of benign and mildly pathogenic species of cattle and buffalo. In a previous study, we identified T .buffeli in blood samples originating from the African buffalo (Syncerus caffer) in the Hluhluwe-iMfolozi Game Park (HIP) and the Addo Elephant Game Park (AEGP) in South Africa. The aim of this study was to characterize the 18S rRNA gene and complete internal transcribed spacer (ITS1-5.8S-ITS2) region of T. buffeli samples, and to establish the phylogenetic position of this species based on these loci. The 18S rRNA gene and the complete ITS region were amplified from DNA extracted from blood samples originating from buffalo in these localities. The PCR products were cloned and the resulting recombinants sequenced. We identified novel T. buffeli 18S rRNA gene and ITS genotypes from buffalo in the AEGP, and novel T. sinensis 18S rRNA genotypes from buffalo in the HIP. Phylogenetic analyses indicated that the T. buffeli-like sequences were similar to T. buffeli sequences from cattle and buffalo in China and India, and the T. sinensis-like sequences were similar to T. sinensis 18S rRNA sequences of cattle and yak in China. There was extensive sequence variation between the novel T.1 buffeli genotypes of the African buffalo and previously described T. buffeli and T. sinensis genotypes. The presence of organisms with T. buffeli-like and T. sinensis-like genotypes in the African buffalo could be of significant importance, particularly to the cattle industry in South Africa as these animals might act as sources of infections to naïve cattle. This is the first report on the characterization of the full-length 18S rRNA gene and ITS region of T. buffeli and T. sinensis genotypes in South Africa. Our study provides invaluable information towards the classification of this complex group of benign and mildly pathogenic species.

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“…Although the application of Giemsa-stained blood smears are suitable for the detection of acute cases, it requires experienced personnel and furthermore is not practical for epidemiological studies. A polymerase chain reaction (PCR) is a recently described molecular technique to detect T. luwenshuni and T. uilenbergi in both transmitting ticks and in the ovine host (Sun et al 2008;Yin et al 2008); however, it is expensive, requires a high degree of expertise, and cannot be used to detect subclinical infection. A reverse line blot (RLB) assay was also developed which specifically identifies different ovine Theileria and Babesia parasites (Schnittger et al 2004;Altay et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Identification of clone-9 antigenic protein of Theileria uilenbergi and evaluation of its application for serodiagnosis

et al. 2010

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The pathogenic protozoan parasite Theileria uilenbergi is one of the causative agents of theileriosis in small ruminants in China. The infection results in great economical losses in the northwest part of China. Efforts are underway to establish an enzyme-linked immunosorbent assay (ELISA) based on a T. uilenbergi immunodominant recombinantly expressed protein using different approaches in order to perform epidemiological studies in the area. In this study, we describe the possible use of the clone-9 protein for this purpose, which was identified as a potential immunogenic piroplasm protein by random sequencing of cDNA library clones followed by bioinformatic analyses. The clone-9 gene was partially recombinantly expressed and used for the development of an indirect ELISA for the detection of circulating antibodies in sera of T. uilenbergi-infected sheep. No cross-reactivity was observed in serum from animals infected with Theileria lestoquardi. The cut-off was calculated at 48.6% positivity using 25 serum samples from uninfected animals. A total of 101 field samples collected from an endemic area in China were used to evaluate the clone-9 ELISA for its use in the field.

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Investigations into the natural infection rate of Haemaphysalis qinghaiensis with Piroplasma using a nested PCR

Cited by 15 publications

References 20 publications

Simultaneous detection of piroplasma infections in field Haemaphysalis qinghaiensis ticks by reverse line blotting

Simultaneous detection of piroplasma infections in field Haemaphysalis qinghaiensis ticks by reverse line blotting

Phylogeny of Theileria buffeli genotypes identified in the South African buffalo (Syncerus caffer) population

Identification of clone-9 antigenic protein of Theileria uilenbergi and evaluation of its application for serodiagnosis

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