Investigations of Acacia modesta Wall. leaves for in vitro anti-diabetic, proliferative and cytotoxic effects

Abstract

The leaves of Acacia modesta Wall. have been shown to possess diverse pharmacological properties. Therefore, we aimed at evaluating anti-diabetic, cytotoxic and proliferative effects of extracts of Acacia modesta Wall. leaves. After evaluating the primary and secondary metabolites, anti-diabetic activity of various extracts was assessed by α-amylase inhibition, glucose uptake by yeast cells and non-enzymatic glycosylation of hemoglobin assay. Cytotoxicity and proliferative potential was assessed by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and short term proliferation assays, respectively, using human liver carcinoma cell line, HepG2. Among other extracts, chloroform extract exhibited 34.16% inhibition of α-amylase, 90.65% inhibition of hemoglobin glycosylation and 94.75% glucose uptake employing α-amylase inhibition, non-enzymatic glycosylation of hemoglobin and glucose uptake by yeast cells assays, respectively. Moreover, extracts exhibited no significant effects on HepG2 cell viability and proliferation. So, this data suggested that chloroform extract of leaves of Acacia modesta Wall., exhibited higher anti-hyperglycemic activity in comparison to extracts in other solvents, while no extract demonstrated cytotoxic and proliferation effects when tested using HepG2 cell line.

Evaluation of Polyphenolic Compounds and Pharmacological Activities in Hairy Root Cultures of Ligularia fischeri Turcz. f. spiciformis (Nakai)

“…The inhibition potential was attained using the dinitrosalicylic acid (DNSA) method slight modifications [43,60]. The trial mixture contained 500 μL of 0.02 M Na 3 PO 4 buffer having α -amylase solution (1 U/mL) sample extracts at 20–100 μg/mL and mixed with 100 mL of starch (1%) and incubated for 20 min at 37 °C.…”

“…Non-enzymatic glycosylation of hemoglobin activity was described earlier with some modifications [60]. Briefly, 1 mL of hemoglobin (0.06%), 5 µL of gentamycin (0.02%), 1 mL sample extracts (1 mg/mL) and 1 mL of glucose solution 0.2% was mixed.…”

Impact of Copper Oxide Nanoparticles on Enhancement of Bioactive Compounds Using Cell Suspension Cultures of Gymnema sylvestre (Retz.) R. Br

“…CuO NP treated and non-treated CSC extracts were carried out the antidiabetic activity. Alpha-amylase inhibition was determined using the dinitrosalicylic acid (DNSA) method [25,34]. The test mixture contained 500 µL of 0.02 M Na 3 PO 4 buffer containing α-amylase solution (1 U/mL) and each CSC extract at 20 to 100 µg/mL mixed with 100 mL of starch (1%).…”

“…Non-enzymatic glycosylation of hemoglobin was determined as previously described [34]. Briefly, 1 mL of hemoglobin (0.06%), 5 µL of gentamycin (0.02%), 1 mL CSC extracts (1 mg/mL), and 1 mL of glucose solution 0.2% were mixed.…”

Impact of Copper Oxide Nanoparticles on Enhancement of Gymnemic Acid and Phenolic Compounds Using Cell Suspension Culture of <em>Gymnema sylvestre</em> (Retz.) R. Br

“…CuO NPs treated and non-treated CSC extracts were carried out antidiabetic activity. Alphaamylase inhibition assay was completed by the dinitrosalicylic acid method [25,34]. Nonenzymatic glycosylation of hemoglobin activity was estimated as described earlier [34].…”