Abstract. The increased interest in subsurface development (e.g., unconventional hydrocarbon, deep geothermal, waste disposal) and the associated (triggered or induced) seismicity calls for a better understanding of the hydro-seismo-mechanical coupling in fractured rock masses. Being able to bridge the knowledge gap between laboratory and reservoir scales, controllable meso-scale in situ experiments are deemed indispensable. In an effort to access and instrument rock masses of hectometer size, the Bedretto Underground Laboratory for Geosciences and Geoenergies (‘Bedretto Lab’) was established in 2018 in the existing Bedretto Tunnel (Ticino, Switzerland), with an average overburden of 1000 m. In this paper, we introduce the Bedretto Lab, its general setting and current status. Combined geological, geomechanical and geophysical methods were employed in a hectometer-scale rock mass explored by several boreholes to characterize the in situ conditions and internal structures of the rock volume. The rock volume features three distinct units, with the middle fault zone sandwiched by two relatively intact units. The middle fault zone unit appears to be a representative feature of the site, as similar structures repeat every several hundreds of meters along the tunnel. The lithological variations across the characterization boreholes manifest the complexity and heterogeneity of the rock volume, and are accompanied by compartmentalized hydrostructures and significant stress rotations. With this complexity, the characterized rock volume is considered characteristic of the heterogeneity that is typically encountered in subsurface exploration and development. The Bedretto Lab can adequately serve as a test-bed that allows for in-depth study of the hydro-seismo-mechanical response of fractured crystalline rock masses.