Investigations of Grape Anther Culture Aiming Haploid Plant Production

Holló, R.; Misik, S.

doi:10.17660/actahortic.2000.528.48

Cited by 4 publications

(2 citation statements)

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“…Somatic embryos and subsequent plant regeneration most probably occurred from somatic tissues rather than microspores in as much as earlier studies using cytological and genetic evidence (Bouquet et al, 1982;Rajasekaran and Mullins, 1983) demonstrated a somatic origin of calli and subsequent plant regeneration. Because embryogenic cultures arose exclusively from somatic tissues including the filament and anther wall, it was unnecessary to stain anthers and determine the developmental stage of microspores as suggested in previous studies (Hollo and Misik, 2000;Krastanova et al, 2000). The culture of intact stamens (anthers with attached filament) was essential for obtaining an embryogenic response.…”

Section: Resultsmentioning

confidence: 99%

Optimizing Initiation and Maintenance of Vitis Embryogenic Cultures

Dhekney¹,

Li²,

Compton³

et al. 2009

horts

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Stamens and pistils from mature grapevines and leaves from in vitro micropropagation cultures were used to optimize parameters influencing somatic embryogenesis in Vitis. Embryogenic competence was dependent on species/variety, explant type and developmental stage, medium composition, and growth regulator concentration. Of varieties evaluated, a greater number produced embryogenic cultures from stamens and pistils (26) compared with leaves (six). Among the different stamen and pistil stages, Stage II and III explants produced the maximum embryogenic response regardless of genotype and medium composition. Of seven culture media tested, the highest embryogenic response was recorded from varieties cultured on MSI (18) and PIV (16) media. Experiments annually repeated over 3 to 10 years demonstrated reproducible results. Highly reliable protocols for somatic embryogenesis were obtained for 29 Vitis species and varieties, including 18 Vitis vinifera varieties, Vitis riparia, Vitis rupestris, Vitis champinii, and eight Vitis hybrids. Embryogenic cultures were maintained on X6 medium for a period of 6 months to 2 years depending on the variety and used in studies involving genetic transformation and transgenic plant regeneration.

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Section: Resultsmentioning

confidence: 99%

Optimizing Initiation and Maintenance of Vitis Embryogenic Cultures

Dhekney¹,

Li²,

Compton³

et al. 2009

horts

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“…Similar results were obtained by Perrin et al, (2004) who approved the efficiency of the « separated floral buds » in inducing somatic embryogenesis on several Vitis rootstocks and cultivars. Hollo and Misik (2000) observed better embryogenesis in Riesling cultivar at mid-and lateuninucleate phases, if anthers were originated from forced canes. While Salunkhe et al, (1999) selected anthers from V. labruscana at three stages of anther maturity (uninucleate, late uninucleate and tetrads) and obtained embryogenic calluses only from anthers with uninucleate pollen.…”

Section: Discussionmentioning

confidence: 78%

Influence of the flower stage and culture medium on the induction of somatic embryogenesis from anther culture in Tunisian grapevine cultivars

et al. 2007

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Aims: The aim of this research is to identify the optimal flower developmental stage, in order to establish an efficient regeneration protocol via somatic embryogenesis in several local grapevine (Vitis vinifera L.) cultivars, using anther culture.Methods and results: Immature anthers sampled on fruity cuttings, at three flower developmental stages were cultured on three media [MS (1962), NN (1969) and CP (1987)] to which several phytohormonal combinations were added. Anthers, sampled at the « separated floral buds » stage, corresponding to the tetrad stage of microsporogenesis revealed to be the most appropriate for somatic embryogenesis compared to anthers sampled at the « separated clusters » and « early bloom » stages. Therefore, a unique medium, CP (1987) supplemented with 9 μM of 2,4-D and 11.35 μM of thidiazuron was established for the induction as well as the development of somatic embryos.Conclusion: The identification of an appropriate flower developmental stage and the optimization of a unique medium allowed establishing a reliable and repetitive regeneration system via somatic embryogenesis, in several local grapevine cultivars.Significance and impact of study: These embryogenic lines would be particularly appropriate as a target for the genetic transformation, directed towards the induction of tolerance to several biotic and abiotic stresses as well as for bioprospecting.

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Vitis

Soneji¹,

Nageswara-Rao²

2010

Wild Crop Relatives: Genomic and Breeding Resources

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Investigations of Grape Anther Culture Aiming Haploid Plant Production

Cited by 4 publications

References 0 publications

Optimizing Initiation and Maintenance of Vitis Embryogenic Cultures

Optimizing Initiation and Maintenance of Vitis Embryogenic Cultures

Influence of the flower stage and culture medium on the induction of somatic embryogenesis from anther culture in Tunisian grapevine cultivars

Vitis

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