Investigations of Phytoconstituents, Antioxidant and Anti-Liver Cancer Activities of Saueda monoica Forssk Extracted by Microwave-Assisted Extraction

Al-Shawi, Ali Abdul Wahid; Hameed, Mustafa F.; Ali, Nashwan Hussein; Hussein, Kawkab A.

doi:10.31557/apjcp.2020.21.8.2349

Cited by 15 publications

(7 citation statements)

References 12 publications

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“…Antioxidant activity According to Al-Shawi et al [26], used DPPH (2,2-Diphenyl1-picrylhydrazyl) method with some modification to examine the scavenger activity of new compounds. Different volumes (10-80) µl of synthesized compounds (4a-d) and vitamin C (1 mg/mL) were added to 200 µl of DPPH (1 mm) in 96 well/plate and left for 30 min in dark place.…”

Section: Antimicrobial Activitiesmentioning

confidence: 99%

Antioxidant and Antimicrobial Activities of Some Novel 2-Thiohydantoin Derivatives

2020

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Four new 2-thiohydantoin derivatives (4a-d) were synthesized from the reaction of maleimide derivatives (3a-d) with phenylisothiocyanate. The structures of synthetic compounds were characterized using Fourier-transform infrared spectroscopy (FT-IR), 1 H-and 13 C-nuclear magnetic resonance (NMR) spectroscopy, and mass spectrometry. The antioxidant activity of the synthesized compounds showed inactive 4b and 4c compounds, moderately active 4a, and strongly active 4d, compared with vitamin C as positive control (IC50 values = 1.380, 1.726, 4.147, 8.085, and 9.826 µM, respectively). Antibacterial and antifungal activities of compounds showed that only compound 4b had an effect on Pseudomonas aeruginosa, Candida albicans, and Aspergillus niger species.

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Section: Antimicrobial Activitiesmentioning

confidence: 99%

Antioxidant and Antimicrobial Activities of Some Novel 2-Thiohydantoin Derivatives

2020

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“…According to Al-Shawi et al [58], ow cytometry analysis identi ed the G1, S, and G2 phases for compounds 5 and 6b. a system with some modi cations.…”

Section: Cell Cyclementioning

confidence: 99%

Anticancer, Antioxidant Activities and Molecular Docking Study of Thiazolidine -4-one and Thiadiazol Derivatives

Alsalim

Nasir

El‐Arabey

et al. 2022

Preprint

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Liver cancer accounts for a major portion of the global cancer burden. In many nations, the prevalence of this condition has risen in recent decades. New series of thaiazolidinones and thaiadiazolidine have been designed, synthesized, and evaluated for potential antioxidant and antihepatocarcinogenic activity. The antioxidant activity of synthesized compounds was evaluated using a DPPH assay. Furthermore, we examined the compounds against HepG2 cells using MTT assay, flow cytometry analysis through the cell cycle, reactive oxygen species, and apoptosis. The result showed that compound 6b has the highest antioxidant activity with IC50 =60.614±0.739 µM. The anticancer activity showed that compounds 5 and 6b have significant toxicity against liver cancer cells HepG2, IC50 values (9.082 and 4.712) µM, respectively. Flow cytometry experiments revealed that compound 5 arrested HepG2 cells in the S process, while compound 6b arrested HepG2 cells in the G1. Compound 6b had a greater reduction in reactive oxygen species and late apoptosis than compound 5. Substantially, compound 5 had affinity energies of -7.6 and -8.5 for Akt and CDK4 proteins, respectively, but compound 6b had affinity energies of -7.8 and -10.1 for Akt1 and CDK4 proteins, respectively. Consequently, compound 6b had lower binding energies than compound 5. In this work, we used multiple bioinformatics methods to shed light on the prospective therapeutic use of these series as novel candidates to target immune cells in the tumor microenvironment of hepatocellular carcinomas such as CD8+ T cells, endothelial cells, and hematopoietic stem cells. The results of antioxidant, anticancer, molecular docking studies, and bioinformatic analysis showed that compound 6b has a potential impact and could be developed for drug discovery with further research.

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“…Furthermore, the heterocyclic structures are the precursor to many anticancer agents on the market. Some studies [15][16][17] demonstrated the importance of 2-thioxo-4imidazolidinone in cancer treatment.…”

Section: Anticancer Activity Of 2-thioxo-4-imidazolidinone Derivativesmentioning

confidence: 99%

Anti-Liver and Anti-Breast Cancer Activities of Novel 2-Thioxo-4-Imidazolidinone Derivatives

AbdulJabar

Al-Shawi

Mutlaq

2021

Preprint

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MTT assay and flowcytometry analysis were used to examine the anti-liver (HepG2) and anti-breast cancer (MCF-7) activities of twelve compounds derived from 2-thioxo-4-imidazolidine. The compounds 5a-h and 7f demonstrated significant anticancer activity against breast cancer cells, while the compounds 5a, ab, and 5d-h demonstrated significant anticancer activity against liver cancer cells, with varying IC50 values as compared to Cisplatin as the positive control. Among these compounds, we chose 5a, 5d, and 5h to detect cell cycle phases and late apoptosis. Compound 5a arrested MCF-7 cells in the S phase, while compound 5d arrested cells in the G1 phase. Compound 5a arrested S phase HepG2 cells, compound 5d arrested S phase cells, and compound 5h arrested G2 phase HepG2 cells. Compound 5a had a higher ratio of late apoptosis than compounds 5d and 5h on both cancer cells. Finally, the development of these compounds as new anti-breast and anti-liver agents warrants further research to understand the mechanism of action, especially against breast cancer.

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Investigations of Phytoconstituents, Antioxidant and Anti-Liver Cancer Activities of Saueda monoica Forssk Extracted by Microwave-Assisted Extraction

Cited by 15 publications

References 12 publications

Antioxidant and Antimicrobial Activities of Some Novel 2-Thiohydantoin Derivatives

Antioxidant and Antimicrobial Activities of Some Novel 2-Thiohydantoin Derivatives

Anticancer, Antioxidant Activities and Molecular Docking Study of Thiazolidine -4-one and Thiadiazol Derivatives

Anti-Liver and Anti-Breast Cancer Activities of Novel 2-Thioxo-4-Imidazolidinone Derivatives

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