Investigations of the total flavonoids extracted from flowers of Abelmoschus manihot (L.) Medic against α-naphthylisothiocyanate-induced cholestatic liver injury in rats

Yan, Jiayin; Ai, Guo; Zhang, Xiaojian; Xu, Hui; Huang, Zheng‐Ming

doi:10.1016/j.jep.2015.06.044

Cited by 47 publications

(23 citation statements)

References 53 publications

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“…The present study demonstrated that sweroside significantly attenuated the down-regulation of Cyp7a1, Cyp8b1, and Hsd3b7, which is consistent with results from previous reports [14,17] . INT-747 further decreased the mRNA expression of Cyp7a1 and Cyp8b1 but reversed the Cyp7b1 expression, as compared with the ANIT group.…”

Section: Discussionsupporting

confidence: 93%

“…Therefore, understanding the role of accumulated hepatic bile acids in cholestasic conditions is crucial to elucidate the underlying mechanism of cholestasis. Alpha-naphthylisothiocyanate (ANIT) is a wellcharacterized cholestatic agent that is widely used to mimic human intrahepatic cholestasis in rodents [12][13][14] . The biochemical and pathological changes induced by ANIT administration in animal models are similar to the symptoms of clinical intrahepatic cholestasis [15] .…”

Section: Introductionmentioning

confidence: 99%

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Sweroside ameliorates α-naphthylisothiocyanate-induced cholestatic liver injury in mice by regulating bile acids and suppressing pro-inflammatory responses

Yang

Gong

et al. 2016

Acta Pharmacol Sin

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Aim: Sweroside is an iridoid glycoside with diverse biological activities. In the present study we investigated the effects of sweroside on α-naphthylisothiocyanate (ANIT)-induced cholestatic liver injury in mice. Methods: Mice received sweroside (120 mg·kg -1 ·d -1 , ig) or a positive control INT-747 (12 mg·kg -1 ·d -1 , ig) for 5 d, and ANIT (75 mg/kg, ig) was administered on d 3. The mice were euthanized on d 5, and serum biochemical markers, hepatic bile acids and histological changes were analyzed. Hepatic expression of genes related to pro-inflammatory mediators and bile acid metabolism was also assessed. Primary mouse hepatocytes were exposed to a reconstituted mixture of hepatic bile acids, which were markedly elevated in the ANIT-treated mice, and the cell viability and expression of genes related to pro-inflammatory mediators were examined. Results: Administration of sweroside or INT-747 effectively ameliorated ANIT-induced cholestatic liver injury in mice, as evidenced by significantly reduced serum biochemical markers and attenuated pathological changes in liver tissues. Furthermore, administration of sweroside or INT-747 significantly decreased ANIT-induced elevation of individual hepatic bile acids, such as β-MCA, CA, and TCA, which were related to its effects on the expression of genes responsible for bile acid synthesis and transport as well as pro-inflammatory responses. Treatment of mouse hepatocytes with the reconstituted bile acid mixture induced significant pro-inflammatory responses without affecting the cell viability. Conclusion: Sweroside attenuates ANIT-induced cholestatic liver injury in mice by restoring bile acid synthesis and transport to their normal levels, as well as suppressing pro-inflammatory responses.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Sweroside ameliorates α-naphthylisothiocyanate-induced cholestatic liver injury in mice by regulating bile acids and suppressing pro-inflammatory responses

Yang

Gong

et al. 2016

Acta Pharmacol Sin

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“…Ech could be play as anti-oxidative and anti-inflammatory agent as it is rich in flavonoids. This explanation is in accord with Yan et al (2015) who reported that decrease the release of NO due to the effect of total flavonoids present in the plant extract.…”

Section: Discussionsupporting

confidence: 92%

Protective effect of Echinochrome against intrahepatic cholestasis induced by alpha-naphthylisothiocyanate in rats

et al. 2020

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Abstract The present study was designed to evaluate the protective effects of echinochrome (Ech) on intrahepatic cholestasis in rats induced by a single (i.p.) injection of alpha-naphthylisothiocyanate (ANIT) (75 mg/kg body weight). The rats were pre-treated orally for 48hr (one dose / 24hr) with Ech (1, 5 and 10 mg/kg body weight) or ursodeoxycholic acid (UDCA) 80 mg/kg body weight drug then, injected with ANIT. ANIT markedly increased serum activities of alanine amino transaminase (ALT), aspartate amino transaminase (AST) and alkaline phosphatase (ALP), which was accompanied by a massive inflammation of epithelial cells on bile duct at 24h after ANIT injection. ANIT also increased the levels of total protein (TP), total bilirubin (TB), direct bilirubin (DB), indirect bilirubin (IB), however decrease albumin content (ALB). In addition ANIT increased hepatic MDA and NO level and decreased GSH level and GST activity. The Ech exerted hepatoprotective and anticholestatic effects as assessed by a significant decrease in the activities of serum AST, ALT and ALP, and the levels of TP, TB, DB and IB as well as liver MDA level and NO level. In conclusion, Ech was found to possess hepatoprotective effect against intrahepatic cholestasis induced by hepatotoxin such as ANIT.

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“…[1][2][3] It is traditionally used to treat many illnesses including chronic renal disease, diabetic nephropathy, mouth ulcers, burns, myocardial and cerebral ischemia injury. [4][5][6][7][8][9][10] The extensive phytochemical and pharmacological investigation of Abelmoschus manihot (L.) Medic revealed the major bioactive compounds to be the flavonoids such as hyperoside, isoquercitrin, myricetin, hibifolin, gossypetin, quercetin and quercetin-3'-O-glucoside. [11,12] In some studies, the commonly methods of separation plants extract are macroporous resin, silica gel, polyamide, sephadex G, and sephadex LH-20.…”

Section: Introductionmentioning

confidence: 99%

Comprehensive separation and identification of chemical constituents from Abelmoschus manihot (L.) medic by polyamide chromatography coupled with high‐performance counter‐current chromatography and preparative liquid chromatography

Fan

Cao

2020

Vietnam Journal of Chemistry

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Three methods (polyamide chromatography coupled with high‐performance counter‐current chromatography (HPCCC) and preparative liquid chromatography) were used to detection and separation of flavonoids from Abelmoschus manihot (L.) Medic. For HPLC analysis, there are main six substances in Abelmoschus manihot (L.) Medic. After polyamide chromatography, the main flavonoids existed in fractions eluted between 50 and 90 % aqueous ethanol. Among these, four fractions of interest from column were separated by HPCCC with the different solvent system. And other two fractions were separated by preparative liquid chromatography. Six high‐purity flavonoids were obtained eventually and identified by MS, 1H and 13C NMR: rutin (1), hyperoside (2), isoquercitrin (3), avicularin (4), quercetin (5), amentoflavone (6). In addition, the substances 4 and 6 for the first time were isolated from Abelmoschus manihot (L.) Medic by HPCCC.

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Investigations of the total flavonoids extracted from flowers of Abelmoschus manihot (L.) Medic against α-naphthylisothiocyanate-induced cholestatic liver injury in rats

Cited by 47 publications

References 53 publications

Sweroside ameliorates α-naphthylisothiocyanate-induced cholestatic liver injury in mice by regulating bile acids and suppressing pro-inflammatory responses

Sweroside ameliorates α-naphthylisothiocyanate-induced cholestatic liver injury in mice by regulating bile acids and suppressing pro-inflammatory responses

Protective effect of Echinochrome against intrahepatic cholestasis induced by alpha-naphthylisothiocyanate in rats

Comprehensive separation and identification of chemical constituents from Abelmoschus manihot (L.) medic by polyamide chromatography coupled with high‐performance counter‐current chromatography and preparative liquid chromatography

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