Investigations on lignins and lignification. XXVII. The enzymic degradation of softwood lignin by white-rot fungi

Ishikawa, Hisao; Schubert, Walter; Nord, F. F.

doi:10.1016/0003-9861(63)90043-2

Cited by 92 publications

(15 citation statements)

References 17 publications

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“…Further degradation of the cleaved rings could lead to early C0 2 production without significant depolymerization. It is, however, clear that this mode of degradation is not solely responsible for decomposition, because various low molecular weight aromatic compounds are also produced Henderson 1955;Higuchi et al 1955;Ishikawa et al 1963). The present study examined the relationship between depolymerization and CO 2 production from the aromatic carbons of lignin.…”

Section: Discussionmentioning

confidence: 85%

Mycelium Binding and Depolymerization of Synthetic¹⁴C-Labeled Lignin During Decomposition byPhanerochaete chrysosporium

Chua¹,

Choi²,

Kirk³

1983

Holzforschung

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Section: Discussionmentioning

confidence: 85%

Mycelium Binding and Depolymerization of Synthetic¹⁴C-Labeled Lignin During Decomposition byPhanerochaete chrysosporium

Chua¹,

Choi²,

Kirk³

1983

Holzforschung

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“…Ihre Untersuchungen zeigten, daß neben einer Demethylierung der Methoxylgruppen eine Einführung von CO-Gruppen in die Seitenkette sowie durch Weißfäulepilze auch eine Verkürzung der Seitenketten, aber auch eine Öffnung der aromatischen Ringe am noch polymeren Lignin stattfinden könnte. Bei niedermolekularen Modellverbindungen wiesen Ishikawa et al (1963), Fukuzumi et al (1969 auch auf die Möglichkeit einer Spaltung der Arylglyceryl-0-Arylether-Bindungen durch ligninabbauende Organismen hin. In neuer Zeit haben Untersuchungen von Nimz (1974), von Yamasaki et al (1976), von Lundquist und Olssen (1977) und Lundquist (1979) mit Hilfe der 13 C-oder 2 H-NMR-Spektroskopie die bisherigen Strukturvorstellungen über die Lignine bestätigt und erhärtet.…”

Section: Introductionunclassified

Untersuchungen des mikrobiellen Ligninabbaues durch¹³C-NMR-Spektroskopie an spezifisch¹³C-angereichertem DHP-Lignin aus Coniferylalkohol

Ellwardt¹,

Haider²,

Ernst³

1981

Holzforschung

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Zusammenfassung DHP-Lignin wurde aus spezifisch ' 3 C-angereichertem Coniferylalkohol (O I3 CH 3 , a-oder ß-l3 C oder l3 C-4) hergestellt und mit verschiedenen Mikroorganismen (Weißfäule-oder Modcrfäulepilz, lignolytischem Bakterium) inkubiert. Von den DHP wurden vor und nach der Inkubation die I3 C-NMRSpektren gemessen. Die Signale der ' 3 C-markierten Atome sind gegenüber denjenigen mit der natürli-chen ' 3 C-Häufigkeit beträchtlich verstärkt, bei den durch Mikroorganismen inkubiertcn DHP treten zusätzliche Signale auf, die den durch Mikroorganismen bewirkten Veränderungen am DHP zugeordnet werden können. Besonders auffällig ist dabei eine Öffnung von aromatischen Ringen, eine Spaltung der Arylalkyletherbindungen und ein Abbau der freien Seitenketten. Weiterhin sind zusätzliche Signale primärer und sekundärer Alkohole sowie von Methylengruppen zu beobachten. Diese Gruppen sind teilweise durch reduktive Prozesse entstanden. Jnvestigation of microbial lignin degradation by I3 CNMR spectroscopy of speciflcally **C-enriched DHP-lignin from coniferyl alcoholSummary DHP-lignin was prcpared from specifically l3 C-enriched coniferyl alcohol (O I3 CH 3 , a-or 0-I3 C or 13 C-4) and incubated with different microorganisms (whiterot or softrot fungi, lignolytic bacterium). The 13 C NMR spcctra of these DHP were measured before and after their incubation. Signals from the 13 C-labeled atoms are considerably intensified compared to those with the natural l3 C-abundance. DHP incubated by microorganisms show additional Signals which can be correlated with modifications in the DHP. Especially remarkable are the partial degradation of the aromatic nuclei, the Splitting of arylalkylether bonds and a degradation of nonpolymerized side chains. Furthermore, additional Signals of primary and secondary alcohol groups and of methylene groups could be observed. These groups are partly formed by reductive processes. Einleitung

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“…5) On the other hand, Trametes hirsuta (formerly Coriolus hirsutus) is a promising fungus for biological treatment because it efficiently degrades lignin at high temperatures, 7) and an efficient transformation system was developed. 8) T. hirsuta secretes a series of lignindegrading enzymes, phenoloxidase, [9][10][11] LiP, 11) and MnP (unpublished result). Two cDNAs and two genomic DNAs coding for allelic forms of phenoloxidase of T. hirsuta have been isolated and characterized.…”

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confidence: 99%

Purification and Characterization of Cellobiose Dehydrogenase from White-Rot BasidiomyceteTrametes hirsuta

Nakagame¹,

Furujyo²,

Sugiura³

2006

Bioscience, Biotechnology, and Biochemistry

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In order to save energy during the pulp making process, we tried to use white-rot basidiomycete, Trametes hirsuta, which degrades lignin efficiently. But a decrease in paper strength caused by cellulolytic activity ruled this out for practical application. Since the cellulolytic activity of the fungus must be decreased, we purified and characterized a cellobiose dehydrogenase (CDH) that was reported to damage pulp fiber. The CDH in the culture filtrate of C. hirsutus was purified by freeze-thawing and chromatographic methods. The pI of the enzyme was 4.2 and its molecular weight was 92 kDa. The optimal temperature and pH of the enzyme were 60-70 C and 5.0 respectively. Since the purified CDH decreased the viscosity of pulp in the presence of Fe(III) and cellobiose, it was shown that the suppression of CDH should be an effective way to reduce cellulose damage.

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Investigations on lignins and lignification. XXVII. The enzymic degradation of softwood lignin by white-rot fungi

Cited by 92 publications

References 17 publications

Mycelium Binding and Depolymerization of Synthetic¹⁴C-Labeled Lignin During Decomposition byPhanerochaete chrysosporium

Mycelium Binding and Depolymerization of Synthetic¹⁴C-Labeled Lignin During Decomposition byPhanerochaete chrysosporium

Untersuchungen des mikrobiellen Ligninabbaues durch¹³C-NMR-Spektroskopie an spezifisch¹³C-angereichertem DHP-Lignin aus Coniferylalkohol

Purification and Characterization of Cellobiose Dehydrogenase from White-Rot BasidiomyceteTrametes hirsuta

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Investigations on lignins and lignification. XXVII. The enzymic degradation of softwood lignin by white-rot fungi

Cited by 92 publications

References 17 publications

Mycelium Binding and Depolymerization of Synthetic14C-Labeled Lignin During Decomposition byPhanerochaete chrysosporium

Mycelium Binding and Depolymerization of Synthetic14C-Labeled Lignin During Decomposition byPhanerochaete chrysosporium

Untersuchungen des mikrobiellen Ligninabbaues durch13C-NMR-Spektroskopie an spezifisch13C-angereichertem DHP-Lignin aus Coniferylalkohol

Purification and Characterization of Cellobiose Dehydrogenase from White-Rot BasidiomyceteTrametes hirsuta

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Mycelium Binding and Depolymerization of Synthetic¹⁴C-Labeled Lignin During Decomposition byPhanerochaete chrysosporium

Mycelium Binding and Depolymerization of Synthetic¹⁴C-Labeled Lignin During Decomposition byPhanerochaete chrysosporium

Untersuchungen des mikrobiellen Ligninabbaues durch¹³C-NMR-Spektroskopie an spezifisch¹³C-angereichertem DHP-Lignin aus Coniferylalkohol