Investigations on the Action of the Iron-Containing Growth Factors, Sideramines; and Iron-Containing Antibiotics, Sideromycins

Burnham, Bruce F.

doi:10.1099/00221287-32-1-117

Cited by 24 publications

(9 citation statements)

References 11 publications

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“…The evidence for such a mechanism is strengthened by the complete inability of either isolated outer membranes or KCN-poisoned whole cells to take up the functional siderophore citrate from Fe-citrate complexes concomitantly. Salmonella typhimurium LT-2 enb-7 and Arthrobacter terregens ATCC 13345 do not produce soluble siderophores and are incapable of obtaining iron from Fe-citrate complexes (3), but they can use a number of siderophores produced by other organisms (8,13,22). The low-affinity iron uptake system of E. coli (15) has been defined by its inability to take iron from nitrilotriacetate, a particularly useful complex for meningococci (4).…”

Section: Discussionmentioning

confidence: 99%

Energy-independent uptake of iron from citrate by isolated outer membranes of Neisseria meningitidis

Simonson¹,

Trivett²,

DeVoe³

1981

Infect Immun

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Cyanide-poisoned Neisseria meningitidis SD1C cells rapidly took up 'Fe from iron-citrate complexes during the first 2 min, after which no further iron was accumulated. ['4C]citrate was not taken up concomitantly with 'Fe by these cells.The 'Fe taken up by the poisoned cells was found in the membrane fraction after cells were broken; 70% of the radioactivity was distributed in the outer membrane, and 30% was in the inner membrane. Isolated outer membranes from iron-starved cells were as capable of iron uptake from citrate as intact cells were. As with whole cells, ['4C]citrate was not taken up by isolated outer membranes. A polyacrylamide gel electrophoresis analysis of the proteins from citrate-dialyzed outer membranes after the uptake of 'Fe revealed that the radioactivity was associated with a major band of 36,500 molecular weight.Other recent evidence has shown that meningococci can use human transferrin-bound iron as t Permanent address: Department of Biology, Pacific Union College, Angwin CA 94508. 50 ng of ferrous ammonium sulfate per ml (final concentration) to a final optical density at 600 nm of 0.3 (model 250 spectrophotometer; Gilford Instrument Laboratories, Ontario, Canada). A 75-ml volume of this suspension was the inoculum for 1,425 ml of NDM.The final culture medium contained less than 10 ng of Fe per ml as contaminating iron before inoculation.Cultures in this iron-limited medium (Fig. 1) were incubated at 37°C, in 5% CO2 in air until division in 547 on August 3, 2020 by guest http://iai.asm.org/ Downloaded from

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Section: Discussionmentioning

confidence: 99%

Energy-independent uptake of iron from citrate by isolated outer membranes of Neisseria meningitidis

Simonson¹,

Trivett²,

DeVoe³

1981

Infect Immun

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“…The great majority of baeterial species (autosequesteric) produce sideramines (BICKEL et al, 1960;BURNHAM, 1963) which faeilitate the transport of iron into the microbial celI. A few bacterial species (anautosequesterie) are unable to synthesize their own sideramine and, unIess supplied with such a material, eannot grow even in the presence of available iron.…”

Section: Alleviation Of Serum Bacteriostasis With Iron-binding Bacmentioning

confidence: 99%

The Role of Iron in Bacterial Infections, with Special Consideration of Host-Tubercle Bacillus Interaction

Kochan

1973

Current Topics in Microbiology and Immunology

213

124

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“…Sideramines, of which Mycobactin is an example, are involved in the chelation and transport into the cell of ferric ions (Burnham, 1962(Burnham, , 1963, but addition of ferrous or ferric ions to our medium (Table 1) had no stimulatory effect on strain 9905. The content of metal ions in a dialysed extract of strain 9905 was therefore measured (semiquantitatively).…”

Section: Further Fractionation Of Growth and Emulsifying Factorsmentioning

confidence: 86%

Growth of Mycobacterium rhodochrous on n‐Decane: a New Growth Factor and Emulsifying Agent

Holdom

Turner²

1969

Journal of Applied Bacteriology

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During the study of the growth of Mycobacteriurn rhadochnous NCIB 9905 in a minimal medium with n-decane as the sole carbon source, a growth factor and a hydrocarbon emulsifying factor were discovered. The factors weie heat stable, were released from cells by autolysis or by physical disruption and functioned independently of one another. Inoculations of < 10' cells/ml of culture medium routinely failed to grow unless an autoclaved cell suspension or purified extract was added. This growth factor requirement was not satisfied by exogenous trace metals, vitamins, amino acids, intermediates of decane metabolism or Mycobactin, but was satisfied by an extract from cells of an unrelated species growing on n-decane. The extract of strain 9905 did not satisfy the sideramine requirement of Arthrobactev JG9 and was not associated with ferric ions. Some evidence was obtained suggesting that the growth factor may be of a new type involving the chelation of calcium ions. DURING THE ISOLATION of hydrocarbon-oxidizing organisms from various soil and water samples it was clear that the predominant bacteria belonged to morphologically related groups including Mycobacterium, Arthrobacter and Nocardia. Most of the strains exhibited considerable variation in daily subcultures and often lost the rapidity of growth observed when originally isolated. These variations included passage of a large proportion of the culture from the smooth tb the rough phase, as shown by the dry wrinkled appearance of isolated colonies on agar media and by the appearance of pellets of cells in liquid culture. The pellets reached 5-10 mm in size and posed a serious problem in the industrial exploitation of the bacteria concerned. With one strain, Mycobacterium rhodochrous NCIB 9905, the inability to initiate growth in a mineral salts medium unless large inocula were used was particularly striking, and the nature of the phenomenon was investigated. Materials and MethodsCultures The hydrocarbon-utilizing organisms used were isolated from spillage area soils in the Oil Works of I.C.I. Heavy Organic Chemicals Division, Billingham, Co. Durham, using enrichment cultivation methods and the medium and carbon source described below. Two specimen cultures were deposited in the National Collection of Industrial Bacteria and designated respectively as Pseudomoms aeruginosa (NCIB 9904) and Myco. rhodochrous (NCIB 9905).

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Investigations on the Action of the Iron-Containing Growth Factors, Sideramines; and Iron-Containing Antibiotics, Sideromycins

Cited by 24 publications

References 11 publications

Energy-independent uptake of iron from citrate by isolated outer membranes of Neisseria meningitidis

Energy-independent uptake of iron from citrate by isolated outer membranes of Neisseria meningitidis

The Role of Iron in Bacterial Infections, with Special Consideration of Host-Tubercle Bacillus Interaction

Growth of Mycobacterium rhodochrous on n‐Decane: a New Growth Factor and Emulsifying Agent

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